Cytochrome P450 2E1 (CYP2E1) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Peptide ELISA: 1/32000 ( Detection Limit).  Western blot: 0.05-0.2 µg/ml. Detects a band of ~50kDa in Human Liver lysates.  Immunohistochemistry on Paraffin Sections: 3-5 µg/ml. In paraffin embedded Human Liver shows ER-like staining in hepatocytes.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Peptide with sequence from the internal region of the protein sequence according to NP_000764.1.
Specificity	Recognizes Human CYP2E1. Other species not tested.
Formulation	Tris saline, pH~7.3 State: Aff - Purified State: Liquid purified IgG fraction Stabilizer: 0.5% BSA Preservative: 0.02% Sodium Azide
Concentration	lot specific
Purification	Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	cytochrome P450 family 2 subfamily E member 1
Database Link	Entrez Gene 1571 Human UniProt ID P05181
Background	The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and biosynthesis. Cytochrome P450 2E1 is induced by ethanol, diabetes and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, and exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
Synonyms	Cytochrome P450 2E1, CYP2E, CYPIIE1, Cytochrome P450-J
Note	Calculated Molecular Weight: 56.8kDa (NP_000764.1).
Reference Data

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