NANOG Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Indirect ELISA: To detect Human Nanog (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human Nanog.  Sandwich ELISA: To detect Human Nanog (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human Nanog (Cat.-No AP01140BT) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Nanog. Western blot: To detect hNanog this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human Nanog is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.  Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human prostate malignant adenocarcinoma. The recommended concentration is 0.5 mg/ml with an overnight incubation at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a non-alcohol soluble red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) E.coli derived, 34.5 kDa recombinant Human Nanog
Specificity	This antibody detects Human Nanog. Other species not tested.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Sterile filtered lyophilized Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing immobilized Human Nanog matrix
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	Nanog homeobox
Database Link	Entrez Gene 79923 Human UniProt ID Q9H9S0
Background	Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.
Synonyms	FLJ12581; FLJ40451; hNanog
Reference Data

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