CD18 (ITGB2) Mouse Monoclonal Antibody [Clone ID: MEM-48]

Specifications

Product Data
Clone Name	MEM-48
Applications	FC, FN, IHC, IP, WB
Recommend Dilution	Flow Cytometry: 2 µg/ml. Func. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Immunoprecipitation: 2 µg/ml. Western Blot: 2 µg/ml.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Leukocytes of a patient suffering from a LGL-type leukemia .
Specificity	Recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin). CD18 is a 90-95 kD type I transmembrane protein expressed on all leukocytes.
Formulation	PBS, pH~7.4 without preservatives State: Azide Free State: Liquid (0.2 µm sterile filtered) purified Ig fraction
Concentration	lot specific
Purification	Protein A Chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit beta 2
Database Link	Entrez Gene 3689 Human UniProt ID P05107
Background	CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA-1, and combines with the alpha M chain to form the integrin Mac-1. IIntegrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.
Synonyms	Integrin beta-2, MFI7
Note	Predicted Molecular Weight: 85 kDa
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, Viral myocarditis

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