Cd8a Rat Monoclonal Antibody [Clone ID: 53-6.7]

Specifications

Product Data
Clone Name	53-6.7
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: ≤  2 µg/106 cells. (Ref.1,6,7,9-15).  Immunohistochemistry (Acetone-Fixed Frozen Sections) (See Ref.2-5). Immunoprecipitation (Ref.1,6).
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Spleen cells or thymocyte membranes
Specificity	This 53-6.7 antibody recognizes the alpha chain of the Murine CD8 hetrerodimer (Mr. 32-34 kDa). In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8a+ cells. The 53-6.7 monoclonal antibody also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2. (Ref. 1-8)
Formulation	PBS containing 0.09% Sodium Azide as preservative Label: Biotin State: Liquid purified Ig fraction
Concentration	lot specific
Conjugation	Biotin
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	CD8 antigen, alpha chain
Database Link	Entrez Gene 12525 Mouse UniProt ID P01731
Background	In the Mouse, CD8 exists in two forms – (i) a CD8 heterodimer composed of an α chain (CD8α) and a β chain (CD8β); and (ii) a homodimer of two α chains. The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8α without CD8β. It has been suggested that CD8+β- T cells mature extrathymically, while development of the CD8α+β+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I-restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells. In vivo and in vitro treatment with the 53-6.7 monoclonal antibody effectively depletes CD8α+ cells.
Synonyms	CD8 alpha chain, CD8A, MAL
Reference Data

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