Vcl (Myc-DDK-tagged) - Mouse vinculin (Vcl)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Products
Vcl (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
VCL (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Vcl - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Purified recombinant protein of Mouse vinculin (Vcl), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
| Tag | C-MYC/DDK |
| Expression Host | HEK293T |
VCL with N-tGFP tag for Focal adherin fiber marking (10ug transfection-grade plasmid)
| Organelle | Focal Adherin Fiber |
| Tag | N-tGFP |
| Vector | pCMV6-AN-GFP |
VCL with N-mBFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
| Organelle | Focal Adherin Fiber |
| Tag | N-mBFP |
| Vector | pCMV6-AN-mBFP |
VCL with N-mRFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
| Organelle | Focal Adherin Fiber |
| Tag | N-mRFP |
| Vector | pCMV6-AN-mRFP |
VCL with N-mGFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
| Organelle | Focal Adherin Fiber |
| Tag | N-mGFP |
| Vector | pCMV6-AN-mGFP |
VCL with N-tRFP tag for Focal adherin fiber marking (10ug transfection-grade plasmid)
| Organelle | Focal Adherin Fiber |
| Tag | N-tRFP |
| Vector | pCMV6-AN-RFP |
VCL CRISPRa kit - CRISPR gene activation of human vinculin
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Vcl CRISPRa kit - CRISPR gene activation of mouse vinculin
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qPCR primer pairs and template standards against Homo sapiens gene VCL
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Mus musculus gene Vcl
Vcl (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
