Sepsecs (Myc-DDK-tagged) - Mouse Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (Sepsecs)
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
- TrueORF®
Sepsecs (Myc-DDK-tagged) - Mouse Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (Sepsecs)
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
SEPSECS (Myc-DDK-tagged)-Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS)
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
Purified recombinant protein of Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS), full length, with N-terminal HIS tag, expressed in E. coli, 50ug
Tag | N-His |
Expression Host | E. coli |
SEPSECS - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
Format | 2 gRNA vectors, 1 linear donor |
Donor DNA | EF1a-GFP-P2A-Puro |
Sepsecs - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
Format | 2 gRNA vectors, 1 linear donor |
Donor DNA | EF1a-GFP-P2A-Puro |
Purified recombinant protein of human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase, Met61-Ser501, with N-terminal His tag, expressed in E.coli, 50ug
Tag | N-His |
Expression Host | E. coli |
Purified recombinant protein of human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase, Lys208-Ser501, with N-terminal His tag, expressed in E.coli, 50ug
Tag | N-His |
Expression Host | E. coli |
SEPSECS CRISPRa kit - CRISPR gene activation of human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Sepsecs CRISPRa kit - CRISPR gene activation of mouse Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qPCR primer pairs and template standards against Homo sapiens gene SEPSECS
Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Mus musculus gene Sepsecs
Sepsecs (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
Purity | HPLC purified |
Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
qSTAR qPCR primer pairs against Homo sapiens gene SEPSECS
Sepsecs (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
Purity | HPLC purified |
Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
SEPSECS - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
Format | Lentiviral plasmids |
Vector | pGFP-C-shLenti |