SHFM1 (Myc-DDK-tagged)-Human split hand/foot malformation (ectrodactyly) type 1 (SHFM1)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
Products
Shfm1 (Myc-DDK-tagged) - Mouse split hand/foot malformation (ectrodactyly) type 1 (Shfm1)
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Sem1 (Mouse) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
SHFM1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRFP-C-RS |
SHFM1 - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Sem1 - KN2.0, Mouse gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
C7orf76 (Myc-DDK tagged) - Homo sapiens chromosome 7 open reading frame 76 (C7orf76), transcript variant 2
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
C7orf76 (Myc-DDK tagged) - Homo sapiens chromosome 7 open reading frame 76 (C7orf76), transcript variant 1
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
SEM1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
SHFM1 HEK293T cell transient overexpression lysate (as WB positive control)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
SEM1 CRISPRa kit - CRISPR gene activation of human SEM1 26S proteasome complex subunit
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Sem1 CRISPRa kit - CRISPR gene activation of mouse SEM1, 26S proteasome complex subunit
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
Transient overexpression lysate of split hand/foot malformation (ectrodactyly) type 1 (SHFM1)
| Tag | C-Myc/DDK |
| Expression Host | HEK293T |
qPCR primer pairs and template standards against Homo sapiens gene SHFM1
| Application | Plasmid of exact quantity for transcript copy number calculation |
Sem1 (Rat) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
