SEPSECS (Myc-DDK-tagged)-Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS)
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
- TrueORF®
Products
Purified recombinant protein of Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS), full length, with N-terminal HIS tag, expressed in E. coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
SEPSECS - KN2.0, Human gene knockout kit via CRISPR, non-homology mediated.
| Format | 2 gRNA vectors, 1 linear donor |
| Donor DNA | EF1a-GFP-P2A-Puro |
Purified recombinant protein of human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase, Met61-Ser501, with N-terminal His tag, expressed in E.coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
Purified recombinant protein of human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase, Lys208-Ser501, with N-terminal His tag, expressed in E.coli, 50ug
| Tag | N-His |
| Expression Host | E. coli |
SEPSECS CRISPRa kit - CRISPR gene activation of human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
| Format | 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug) |
qPCR primer pairs and template standards against Homo sapiens gene SEPSECS
| Application | Plasmid of exact quantity for transcript copy number calculation |
qSTAR qPCR primer pairs against Homo sapiens gene SEPSECS
SEPSECS - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
| Format | Lentiviral plasmids |
| Vector | pGFP-C-shLenti |
SEPSECS - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided
| Format | Retroviral plasmids |
| Vector | pRS |
SEPSECS (tGFP-tagged) - Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS)
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
- TrueORF®
Lenti ORF clone of Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS), Myc-DDK-tagged
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
- LentiORF®
Lenti ORF clone of Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS), mGFP tagged
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Vector | pLenti-C-mGFP-P2A-Puro |
- LentiORF®
SEPSECS (untagged)-Human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (SEPSECS)
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
SEPSECS (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
| Purity | HPLC purified |
| Number of Transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
