Proteins

Proteins

Recombinant Proteins

CytoSections

Mass Spec Standards

Native Proteins

Over-expression Lysates

Human

Mouse

Humon

BDNF

FGFR1

B3GALT5

M-CSF

ASPARTATE BETA HYDROXYLASE

TNF ALPHA

CD10

CD36

GM CSF RECEPTOR ALPHA

ICAM2

ZFYVE27

CD147

CUTA

EGFR

ERBB 3

HER2

SYNTENIN

TRKB

CEACAM1

IL8

CD44

FXYD3

LAMP2

NRG1

PD-L1

ROR1

TMEM169

CD32B

CD33

CD3E

CD47

GAA

HEME OXYGENASE 2

NEUROLIGIN 4

SIGIRR

SIRP ALPHA

BAP31

C20ORF30

CD83

FXYD2

FXYD6

GPR56

HFE

ICAM1

IL22

IL5RA

INPP4A

LRRC8A

MAL

MIDKINE

NEURONAL MEMBRANE GLYCOPROTEIN M6 A

PTPRN

PTPRS

SEMA4B

SYNAPTOTAGMIN 1

TMPRSS4

TRKC

ACID PHOSPHATASE

AIF

CD105

CD200

CD200R

CD98

CD99L2

DPP6

FAM3C

FCGRT

GOLPH2

JUNCTIONAL ADHESION MOLECULE 1

SONIC HEDGEHOG

CCK4

CD84

CLM 9

CTLA4

CYSTATIN C

FGFR3

HAPTOGLOBIN

IL6R

LTA

LY108

MCK10

MET

PD L2

PD1

SYNAPTOTAGMIN 3

TMEM51

VCAM1

ACBD4

AMYLOID PRECURSOR PROTEIN

ASIALOGLYCOPROTEIN RECEPTOR 2

B7H3

CD14

CD272

CD79B

CD89

DDR2

DR5

GLEPP1

ICOS LIGAND

IL1 RECEPTOR II

IHC

WB

HEK293T

HEK293

E. coli

Sf9

Insect

CHO

Baculovirus

Human

C-Myc/DDK

C-MYC/DDK

C-His

N-His

C-Fc

Tag Free

C-DDK/His

C-6His

N-GST and C-His

C-DDK

N-GST and C-HIS

C-mFc

C-Fc&His

C-6His-Avi

N-His-ABP (Albumin-Binding Protein)

N-Trx&His

C-HIS

C-6×His

C-Fc-Avi

C-Human Fc

C-hFc

His-tag

C-Mouse Fc

Myc-DDK

C-8His

N-6His

N-His-Trx

N-GST

C-Mouse Fc and 6×His

C-Avi-6His

N-Fc

C- 6×His

C-10His

C-Fc&AVI

C-Human Fc and 6×His

N-Trx

C-Fc/His

C-hFc&His

His-Tag

N-GST, C-His

N-His/DDK

C-Flag

C-HUMAN

N-6His-Avi

N-Mouse Fc and C-6×His

Tag-free

C-6xHis/AVI

C-AVI&His

C-FC

C-FC-6HIS&C-FC-Flag

C-FLAG-Fc

C-GST

C-His/DDK

C-hIgG2 Fc

C-hIgG4 Fc

C-mFC

N-6His-Flag

N-8His

N-8His&N-FLAG

N-Avi&6His

N-DDK/His

N-FC

N-Flag&His

N-GFP

N-His PDCD1(Pro21-Val170)

N-His-GST

N-TRX

N-TRXA

N-Trx-His

N-mFc

MVPro

CytoSections

SDS PAGE

WB

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Ion Channels: Other

Adult stem cells

GPCR

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Potassium

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Cys-loop Receptors

ELISA

SDS-PAGE

Ion Channels: Glutamate Receptors

Ion Channels: Transient receptor potential

Ion Channels: ATP Receptors

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Ion Channels: Sodium

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

Ion Channels: Cyclic nucleotide gated

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Ion Channels: Calcium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Cytokine-cytokine receptor interaction

Metabolic pathways

Cell adhesion molecules (CAMs)

Hematopoietic cell lineage

Pathways in cancer

Neuroactive ligand-receptor interaction

MAPK signaling pathway

Natural killer cell mediated cytotoxicity

Endocytosis

Calcium signaling pathway

Regulation of actin cytoskeleton

Jak-STAT signaling pathway

Focal adhesion

Axon guidance

Adherens junction

Alzheimer's disease

Leukocyte transendothelial migration

Lysosome

Dilated cardiomyopathy

Hypertrophic cardiomyopathy (HCM)

ECM-receptor interaction

Antigen processing and presentation

Toll-like receptor signaling pathway

Systemic lupus erythematosus

Type I diabetes mellitus

Viral myocarditis

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Chemokine signaling pathway

Apoptosis

TGF-beta signaling pathway

Adipocytokine signaling pathway

Melanoma

Allograft rejection

Drug metabolism - cytochrome P450

Prostate cancer

Graft-versus-host disease

Colorectal cancer

Olfactory transduction

Retinol metabolism

T cell receptor signaling pathway

Autoimmune thyroid disease

Epithelial cell signaling in Helicobacter pylori infection

ErbB signaling pathway

SNARE interactions in vesicular transport

PPAR signaling pathway

Huntington's disease

Neurotrophin signaling pathway

Primary immunodeficiency

Metabolism of xenobiotics by cytochrome P450

Pancreatic cancer

Glycosphingolipid biosynthesis - lacto and neolacto series

Androgen and estrogen metabolism

Tight junction

Asthma

N-Glycan biosynthesis

Starch and sucrose metabolism

Melanogenesis

Bladder cancer

Drug metabolism - other enzymes

Purine metabolism

B cell receptor signaling pathway

Porphyrin and chlorophyll metabolism

Amyotrophic lateral sclerosis (ALS)

Oxidative phosphorylation

Parkinson's disease

Cardiac muscle contraction

Glycerophospholipid metabolism

RIG-I-like receptor signaling pathway

Sphingolipid metabolism

Fatty acid metabolism

Gap junction

O-Glycan biosynthesis

Ascorbate and aldarate metabolism

Pyrimidine metabolism

Arachidonic acid metabolism

NOD-like receptor signaling pathway

Basal cell carcinoma

Glioma

Keratan sulfate biosynthesis

Endometrial cancer

Pentose and glucuronate interconversions

Vascular smooth muscle contraction

Fc gamma R-mediated phagocytosis

GnRH signaling pathway

Wnt signaling pathway

Fc epsilon RI signaling pathway

Heparan sulfate biosynthesis

Renin-angiotensin system

Hedgehog signaling pathway

p53 signaling pathway

Complement and coagulation cascades

Linoleic acid metabolism

Non-small cell lung cancer

Pathogenic Escherichia coli infection

Glycosphingolipid biosynthesis - globo series

Tyrosine metabolism

Vibrio cholerae infection

Small cell lung cancer

Chronic myeloid leukemia

Renal cell carcinoma

Nicotinate and nicotinamide metabolism

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Riboflavin metabolism

ABC transporters

Chondroitin sulfate biosynthesis

Notch signaling pathway

Long-term depression

Cytosolic DNA-sensing pathway

Type II diabetes mellitus

Taste transduction

Ether lipid metabolism

Glutathione metabolism

Glycerolipid metabolism

Nitrogen metabolism

VEGF signaling pathway

Glycosphingolipid biosynthesis - ganglio series

Steroid biosynthesis

Tryptophan metabolism

Glycosaminoglycan degradation

Prion diseases

Long-term potentiation

Amino sugar and nucleotide sugar metabolism

C21-Steroid hormone metabolism

Galactose metabolism

Insulin signaling pathway

Phosphatidylinositol signaling system

Thyroid cancer

Acute myeloid leukemia

Caffeine metabolism

alpha-Linolenic acid metabolism

Inositol phosphate metabolism

Dorso-ventral axis formation

Progesterone-mediated oocyte maturation

Ubiquitin mediated proteolysis

beta-Alanine metabolism

Primary bile acid biosynthesis

Biosynthesis of unsaturated fatty acids

Glycine

Lysine degradation

Propanoate metabolism

Valine

leucine and isoleucine degradation

serine and threonine metabolism

Cell cycle

Oocyte meiosis

Other glycan degradation

Glycolysis / Gluconeogenesis

Arginine and proline metabolism

Histidine metabolism

Limonene and pinene degradation

Pantothenate and CoA biosynthesis

Phenylalanine metabolism

Butanoate metabolism

Pyruvate metabolism

Sulfur metabolism

Protein export

Cyanoamino acid metabolism

Selenoamino acid metabolism

Taurine and hypotaurine metabolism

Base excision repair

Fatty acid elongation in mitochondria

O-Mannosyl glycan biosynthesis

Citrate cycle (TCA cycle)

Terpenoid backbone biosynthesis

Human

Human 293

E. coli

CHO Stable

HEK293

Mammalian

Neutrophils

P.Pichia

1

2

Yes

In Stock