Proteins

Proteins

Recombinant Proteins

CytoSections

Mass Spec Standards

Mutant Proteins

Over-expression Lysates

Human

HMGA1

MTH1

AURORA A

ANNEXIN A2

ERBB 3

P53

HMG1

TGIF

APE1

BAT3

APOLIPOPROTEIN E

DAXX

FBP1

NUCLEOPHOSMIN

PPM1B

DNA POLYMERASE DELTA P50

NM23A

SHP1

BIRC5

LAP2

MATK

CTBP2

DIAPH1

EXOSC3

IKB BETA

MCM4

NOLA1

NUP50

PCNA

POLR1D

RAD54

RETINAL PROTEIN 4

RFC4

SMN1

ANNEXIN A3

BNP

CD168

CYCLOPHILIN 40

FKBP25

GADD45A

HPRT

KPNA2

LSM1

RFC2

SELENOPHOSPHATE SYNTHETASE 1

SNRPN

BCL2

C-MYC

CD30

CHK1

OCT4

PAXILLIN

RAD51

BCCIP

CARDIAC TROPONIN I

CASPASE 9

CYCLIN D1

MSH2

PCYT1B

PHGDH

SF2

SNRPB

STIP1

SUMO 1

UNG

ALPHA TUBULIN

CD117

CDC25C

CDK1

CHK2

DAZAP1

DNMT3B

EWSR1

GEMIN 2

GRB7

HNRNP Q

HSC70

HSPA14

KAP1

LCK

LSM4

MNAT1

MRE11

NTH1

PARP1

POLR1C

PRMT5

PSMA3

PSME3

RFC3

RPP20

SIGLECL1

SYNTAXIN 3

ARMC6

ATIC

AURORA B

B MYB

BUBR1

BYSL

CAF1

IHC

WB

HEK293T

HEK293

E. coli

Sf9

C-Myc/DDK

C-MYC/DDK

N-His

C-His

Tag Free

C-DDK

Myc-DDK

N-GST and C-His

C-DDK/His

N-His&C-His

N-MARI

C-Fc

N-GST and C-HIS

C-6His

C-6His-Avi

C-mFc

His-tag

C-6×His

C-HIS

N-DDK

N-His&Flag

N-His-ABP (Albumin-Binding Protein)

N-His-GST

Yes

MVPro

CytoSections

SDS PAGE

WB

Druggable Genome

Transmembrane

Transcription Factors

Secreted Protein

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Ion Channels: Other

Adult stem cells

GPCR

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Ion Channels: Potassium

Stem cell relevant signaling - TGFb/BMP signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - Wnt Signaling pathway

Ion Channels: Cys-loop Receptors

ELISA

SDS-PAGE

Ion Channels: Glutamate Receptors

Ion Channels: Transient receptor potential

Ion Channels: ATP Receptors

Bioactivity

the solution can be further dialyzed against PBS (phosphate buffered saline

10 mM Tris-HCl

2 mM dithiothreitol

Ion Channels: Sodium

Enzyme Activity

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

ELISA.
Western Blot.

Flow Cyt

Ion Channels: Cyclic nucleotide gated

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.

pH 7.4).
For immunization purposes

Ion Channels: Calcium

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

e.g. Dulbeccos PBS).

e.g. Dulbeccos PBS).
See References 2 and 3 for more details.

pH 7.4).
For immunization purposes

pH 7.4).
For Immunization purposes

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

Can be used as standard in a Sandwich ELISA.

FACS

Protein standard in 1D and 2D SDS gel electrophoresis.
Immunoassay.
Antigen for immunization.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

Suitable for Immunoblotting (Western or Dot blot)

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/32

000-1/40

000.

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western Blot: 1/500-1/2

000.
Western blot: 1/10

500.
Immunohistochemistry: 1/200-1/1

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Suitable for use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

ELISA.
Western Blot.

ELISA.
Western blotting.

ELISA: this protein is suitable for use as a Mouse IgE standard in ELISA assays.

ELISA.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA and Immunohistochemistry as well as other beta galactosidase-avidin/biotin based enzymatic assays. This product has been assayed against 1.0 µg of biotinylated IgG in a standard capture ELISA using OPNG as a substrate for 30 minutes at room temperature.
Recommended Dilutions:
ELISA: 1/8000-1/32000.
Western Blot: 1/500-1/2500.
Immunohistochemistry: 1/200-1/1000.

ELISA and immunohistochemistry as well as other glucose oxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/8

ELISA.
Functional Assays.

ELISA.
Western blotting.

For use as a Control or Standard in Indirect trapping ELISA for quantitation of antigen in serum using a standard curve

For use as a Control or standard in indirect trapping ELISA for quantitation of antigen in serum using a standard curve.
Immunoprecipitation.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Specific methodologies have not been tested using this product.

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

change growth medium against basal medium (EBM) in the early afternoon]
- change EGF against EBM (for HUVEC: EBM +1-2% FCS)
- incubate 24 h
- change medium again and add factors (growth factors

etc.)
- incubate for 18 h
- add 10 µl 3H-Thymidine solution [0.025 mCi/ml] per well (=0.25 µCi)
- incubate another 6h at 37°C
- Washing steps: (250 µl/well)
PBS 1x
MeOH 2x 5 min
TCA 2x 10 min
H2O 1x
- lyse cells in 250 µl 0.3M NaOH per well
- transfer 2.5 ml ECO Plus into the appropriate scintillation vials
- transfer cell lysats into the scintillation vials
- count by liquid scintillation (ß-counter; Beckmann Instruments)

for immunoprecipitation and for Wwestern blotting.

inhibitors

optimal concentration with heparin (50 μg/ml) is about 12 μg/ml.
Protocol: Thymidine Incorporation with HUVEC
- plate cells with a density at 5-7 x 10e3 cells/well in growth medium (EGM)
- incubate cells over night [if urgent

plate cells in the morning

Cell cycle

Metabolic pathways

Pathways in cancer

DNA replication

Base excision repair

MAPK signaling pathway

Spliceosome

p53 signaling pathway

Nucleotide excision repair

Mismatch repair

Colorectal cancer

Purine metabolism

RNA degradation

Small cell lung cancer

Pyrimidine metabolism

Chronic myeloid leukemia

Homologous recombination

Amyotrophic lateral sclerosis (ALS)

Prostate cancer

Wnt signaling pathway

Oocyte meiosis

Focal adhesion

Huntington's disease

Endometrial cancer

Pancreatic cancer

Apoptosis

Jak-STAT signaling pathway

Neurotrophin signaling pathway

Endocytosis

T cell receptor signaling pathway

Bladder cancer

Thyroid cancer

ErbB signaling pathway

Non-small cell lung cancer

Regulation of actin cytoskeleton

Calcium signaling pathway

Progesterone-mediated oocyte maturation

Cytosolic DNA-sensing pathway

Glioma

Melanoma

Natural killer cell mediated cytotoxicity

B cell receptor signaling pathway

Chemokine signaling pathway

Alzheimer's disease

Antigen processing and presentation

Proteasome

Acute myeloid leukemia

Drug metabolism - other enzymes

Insulin signaling pathway

Basal cell carcinoma

Viral myocarditis

Systemic lupus erythematosus

Notch signaling pathway

Parkinson's disease

RNA polymerase

Fructose and mannose metabolism

Glycolysis / Gluconeogenesis

NOD-like receptor signaling pathway

One carbon pool by folate

Pentose phosphate pathway

VEGF signaling pathway

Adherens junction

Cytokine-cytokine receptor interaction

Primary immunodeficiency

Ubiquitin mediated proteolysis

Gap junction

Leukocyte transendothelial migration

Non-homologous end-joining

Axon guidance

SNARE interactions in vesicular transport

Adipocytokine signaling pathway

Fc gamma R-mediated phagocytosis

Pathogenic Escherichia coli infection

RIG-I-like receptor signaling pathway

ECM-receptor interaction

Selenoamino acid metabolism

TGF-beta signaling pathway

Glycerophospholipid metabolism

Cardiac muscle contraction

Cysteine and methionine metabolism

Dilated cardiomyopathy

Glycine

Hypertrophic cardiomyopathy (HCM)

Prion diseases

serine and threonine metabolism

Hematopoietic cell lineage

Melanogenesis

Renal cell carcinoma

Epithelial cell signaling in Helicobacter pylori infection

Folate biosynthesis

Glyoxylate and dicarboxylate metabolism

Nicotinate and nicotinamide metabolism

Steroid biosynthesis

Type II diabetes mellitus

Type I diabetes mellitus

Alanine

Arginine and proline metabolism

Basal transcription factors

Ether lipid metabolism

Glycerolipid metabolism

Phenylalanine

Phenylalanine metabolism

Sphingolipid metabolism

Tyrosine metabolism

aspartate and glutamate metabolism

tyrosine and tryptophan biosynthesis

Human

E. coli

Human 293

1

2

Yes

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