Recombinant Proteins

Proteins

Recombinant Proteins

Human

Mouse

ALPHA 1 ANTITRYPSIN

FGF1

TNF ALPHA

VEGFA

BETA 2 MICROGLOBULIN

EGFR

IGF1

IL8

M-CSF

ERBB 3

FSTL1

GM CSF

IL4

ANNEXIN A2

FGL1

IL1RA

IL2

IL6

OSTEOPONTIN

PCSK9

APOLIPOPROTEIN E

G CSF

GM CSF RECEPTOR ALPHA

IL1 BETA

IL17

IL22

INTERFERON GAMMA

KALLIKREIN 11

KALLIKREIN 6

APOLIPOPROTEIN A I

BDNF

CALCITONIN

SDF1

SONIC HEDGEHOG

ADIPONECTIN

CLUSTERIN

DECORIN

FGF2

IL18

IL33

INTERFERON ALPHA 2

NGF

REG3G

ANGIOTENSIN CONVERTING ENZYME 2

CCL14

CXCL5

FAM3C

HAPTOGLOBIN

HYAL1

IL1F10

LTA

NGAL

NRG1

PLAUR

PREALBUMIN

REG3A

SERPING1

TGF BETA 1

APOLIPOPROTEIN H

CD95

EFEMP1

EOTAXIN 3

G PROTEIN ALPHA S

IL15

IL5

IL7

INHIBIN BETA C CHAIN

KALLIKREIN 8

KGF

LIF

MAGP1

MCP2

MIDKINE

MMP1

MTMR14

ONCOSTATIN M

PAI1

PIGR

RAB37

SYNAPTOTAGMIN 1

SYNAPTOTAGMIN 3

VITRONECTIN

ALPHA 2 ANTIPLASMIN

ARMET

BMP2

BNP

CCL3

CXCL7

FGF21

FGFR2

FLT1

FSH BETA

IGFBP5

IL12B

IL1RAP

IL21

IL3

IL37

LEPTIN

MESOTHELIN

HEK293T

E. coli

HEK293

Insect

CHO

Sf9

Pichia

Baculovirus

C-Myc/DDK

C-His

Tag Free

N-His

C-6His

C-Fc

N-GST and C-His

N-GST and C-HIS

C-DDK

C-HIS

C-Fc&His

C-DDK/His

His-tag

C-8His

Myc-DDK

N-GST

C-Human Fc

N-His-Trx

C-10His

C-mFc

N-6His

C-6His-Avi

N-His-ABP (Albumin-Binding Protein)

Tag-free

C-6×His

N-Avi

N-Fc

C-Avi-6His

C-hFc

His-Tag

N-His/DDK

C-MYC/DDK

N-8His

N-His-PDCD1(Pro21-Val170)

C-6xHis/AVI

C-AVI

C-AVI&His

C-Avi

C-Avi&6His

C-Avi-10His

C-DDK/10xHis

C-DDK/6xHis

C-FC

C-FLAG-Fc

C-Fc-Avi

C-Fc/His

C-Flag,C-6His

C-His&Flag

C-His&HA&AVI

C-Human Fc and 6×His

C-Mouse Fc

C-hIgG2 Fc

C-hIgG4 Fc

C-terminal His

N-6His-Avi

N-AVI&His

N-DDK/His

N-FLAG

N-Fc-FLAG

N-Flag&His

N-HIS

N-His&C-His

N-His&Flag

N-T7

N-Trx-His

N-hFc

N-mFc

N-terminal His

N-terminal His-PDCD1(Pro21-Val170) tag

Tag free

Yes

MVPro

SDS PAGE

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

GPCR

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

ELISA

Stem cell relevant signaling - Wnt Signaling pathway

SDS-PAGE

Ion Channels: Potassium

Bioactivity

Ion Channels: Glutamate Receptors

the solution can be further dialyzed against PBS (phosphate buffered saline

Ion Channels: ATP Receptors

Ion Channels: Cys-loop Receptors

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

e.g. Dulbecco's PBS).

Flow Cyt

ELISA.
Western Blot.

Ion Channels: Transient receptor potential

Ion Channels: Sodium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cyclic nucleotide gated

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/40

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western blot: 1/10

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Western blotting.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Functional Assays.

ELISA.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

Cytokine-cytokine receptor interaction

Pathways in cancer

Jak-STAT signaling pathway

MAPK signaling pathway

Hematopoietic cell lineage

Chemokine signaling pathway

Toll-like receptor signaling pathway

Complement and coagulation cascades

Focal adhesion

Regulation of actin cytoskeleton

Natural killer cell mediated cytotoxicity

Melanoma

TGF-beta signaling pathway

T cell receptor signaling pathway

NOD-like receptor signaling pathway

Autoimmune thyroid disease

Type I diabetes mellitus

Allograft rejection

RIG-I-like receptor signaling pathway

Cytosolic DNA-sensing pathway

Metabolic pathways

Fc epsilon RI signaling pathway

Graft-versus-host disease

Bladder cancer

Apoptosis

Antigen processing and presentation

Systemic lupus erythematosus

Asthma

Pancreatic cancer

Hypertrophic cardiomyopathy (HCM)

GnRH signaling pathway

Endocytosis

Dilated cardiomyopathy

Alzheimer's disease

ECM-receptor interaction

PPAR signaling pathway

Prion diseases

Renal cell carcinoma

mTOR signaling pathway

Regulation of autophagy

Neuroactive ligand-receptor interaction

Adipocytokine signaling pathway

Hedgehog signaling pathway

Prostate cancer

Basal cell carcinoma

p53 signaling pathway

Epithelial cell signaling in Helicobacter pylori infection

Long-term depression

Melanogenesis

ErbB signaling pathway

Wnt signaling pathway

Axon guidance

Calcium signaling pathway

VEGF signaling pathway

Type II diabetes mellitus

Colorectal cancer

Glioma

Neurotrophin signaling pathway

Renin-angiotensin system

Ether lipid metabolism

Vascular smooth muscle contraction

Amyotrophic lateral sclerosis (ALS)

Glycerolipid metabolism

Cell cycle

Gap junction

Arachidonic acid metabolism

Cell adhesion molecules (CAMs)

Primary immunodeficiency

Glycerophospholipid metabolism

Small cell lung cancer

Linoleic acid metabolism

alpha-Linolenic acid metabolism

Chronic myeloid leukemia

Oocyte meiosis

Progesterone-mediated oocyte maturation

Adherens junction

Dorso-ventral axis formation

Endometrial cancer

Lysosome

Non-small cell lung cancer

Fc gamma R-mediated phagocytosis

Proteasome

Glycosaminoglycan degradation

Huntington's disease

Leukocyte transendothelial migration

Histidine metabolism

Viral myocarditis

O-Glycan biosynthesis

Taste transduction

Vibrio cholerae infection

Acute myeloid leukemia

Amino sugar and nucleotide sugar metabolism

Starch and sucrose metabolism

N-Glycan biosynthesis

beta-Alanine metabolism

Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Maturity onset diabetes of the young

Other glycan degradation

Pathogenic Escherichia coli infection

Purine metabolism

Pyrimidine metabolism

SNARE interactions in vesicular transport

Glutathione metabolism

Galactose metabolism

Arginine and proline metabolism

Biotin metabolism

Folate biosynthesis

Glycosphingolipid biosynthesis - ganglio series

Glycosphingolipid biosynthesis - globo series

Glycosphingolipid biosynthesis - lacto and neolacto series

Insulin signaling pathway

Keratan sulfate biosynthesis

Nitrogen metabolism

Riboflavin metabolism

Tryptophan metabolism

Tyrosine metabolism

Arrhythmogenic right ventricular cardiomyopathy (ARVC)

Human

E. coli

Human 293

Baculovirus

HEK293

Mammalian

P.Pichia

1

2

Yes

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