Recombinant Proteins

Proteins

Recombinant Proteins

Human

FGFR1

EGFR

ERBB 3

HER2

AURORA A

B RAF

ROR1

JNK1

TRKB

AKT1

FGFR3

MET

TRKC

CAMK2B

CCK4

JNK2

TIE1

CAMK2D

CAMKK

CSNK1G3

FYN

MCK10

NEK6

P38

PAK4

PDGF RECEPTOR BETA

PROTEIN KINASE D2

PYK2

ACTIVIN RECEPTOR TYPE IIA

ACTIVIN RECEPTOR TYPE IIB

BMPR2

DDR2

EPH RECEPTOR A2

FGFR2

FLT1

MCSF RECEPTOR

PKC EPSILON

SRC

ACTIVIN RECEPTOR TYPE IA

CAMK1D

CDK2

ERBB 4

ERK2

HSP22

JNK3

ROR2

RYK

TGF BETA RECEPTOR II

ABL2

ACVRL1

AKT3

AXL

BLK

BMX

CAMK2A

CAMKK2

CDK6

CK1 EPSILON

CSK

DCAMKL1

DYSTROPHIA MYOTONICA PROTEIN KINASE

EPH RECEPTOR A1

EPH RECEPTOR A3

EPH RECEPTOR A7

FAK

FGFR4

FLT4

IGF1 RECEPTOR

KDR

MAPKAP KINASE 2

MATK

MEK3

MSK1

PCTAIRE1

PKC BETA 1

PKC ZETA

PRKACA

RIOK1

STK16

TRKA

TYRO3

ZAK

ALK

BMPR1A

CD117

CSNK1G2

EPH RECEPTOR B3

EPH RECEPTOR B4

FASTK

FLT3

IRE1

MERTK

PDGF RECEPTOR ALPHA

RAF1

VEGF RECEPTOR 1

VEGF RECEPTOR 2

ACTIVIN A RECEPTOR TYPE IC

BTK

CAMK2G

CAMKI

HEK293T

E. coli

Sf9

HEK293

Insect

Baculovirus

CHO

C-Myc/DDK

N-His

C-His

C-DDK/His

C-DDK

C-Fc

N-GST and C-His

N-GST and C-HIS

His-tag

C-Fc&His

N-His-ABP (Albumin-Binding Protein)

C-6His-Avi

N-GST

C-6His

C-6×His

Tag Free

C- 6×His

C-Fc-Avi

C-hFc

C-mFc

Myc-DDK

N-10His-Flag

N-FUSION

N-GST/C-His

Yes

MVPro

SDS PAGE

Druggable Genome

Transmembrane

Secreted Protein

Transcription Factors

Protein Kinase

ES Cell Differentiation/IPS

Protease

Stem cell - Pluripotency

Phosphatase

Adult stem cells

Ion Channels: Other

Embryonic stem cells

Nuclear Hormone Receptor

P450

Cancer stem cells

GPCR

Induced pluripotent stem cells

Stem cell relevant signaling - JAK/STAT signaling pathway

Stem cell relevant signaling - DSL/Notch pathway

Stem cell relevant signaling - TGFb/BMP signaling pathway

ELISA

Stem cell relevant signaling - Wnt Signaling pathway

SDS-PAGE

Ion Channels: Potassium

Bioactivity

Ion Channels: Glutamate Receptors

the solution can be further dialyzed against PBS (phosphate buffered saline

Ion Channels: ATP Receptors

Ion Channels: Cys-loop Receptors

10 mM Tris-HCl

2 mM dithiothreitol

Enzyme Activity

e.g. Dulbecco's PBS).

Flow Cyt

ELISA.
Western Blot.

Ion Channels: Transient receptor potential

Ion Channels: Sodium

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

pH 7.4). For immunization purposes

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

pH 7.4).
For immunization purposes

pH 7.4).
For immunization purposes

Ion Channels: Cyclic nucleotide gated

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoblotting.
Receptor-binding studies.

Western Blot.

0.1% (w/v) insulin

000-1/5

10 mM sodium phosphate

10mM Tris-HCl

1mM DTT

2 mM dithiothreitol

2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25°C for 20 minutes.
4. Record the increase in A650nm for 15 minutes

2mM dithiothreitol

63mM sodium phosphate

Biological activity/ Working concentration: The working concentration of ECGF for HUVEC is in the range of 25µg/ml to 100µg/ml. When adding Heparin (2.5mg per mg ECGF) an ECGF concentration of 12µg/ml (30µg/ml Heparin) is optimal. In this case 6mg ECGF are sufficient for 500 ml medium.

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: After vimentin is dissolved in 9.5M Urea buffer (see above)

Can be used as standard in a Sandwich ELISA.

ELISA.

FACS

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after desmin is dissolved in 9.5 M urea buffer (see above)

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments: after vimentin is dissolved in 6 M urea buffer (see above)

Protocol: 1. Uehara Y.

Protocol: Activity Assay
1. Prepare a 1 ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris

and Fukazawa H. (1991) Methods Enzymol. 201: 370.
2. Fukazawa H.

and Nuccitelli R. (1992) J.Biol.Chem. 267:5608.

both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbeccos PBS).

e.g.Dulbecco's PBS).

et al. (1991) Biochem. Pharmacol. 42: 1661.
3. Satoh T.

et al.(1992) J.Biol.Chem. 267: 2537.
4. Weiss R.

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M Urea and then to low salt condition (50mM NaCl

protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50 mM NaCl

the solution can be further dialyzed against PBS (Phosphate Buffered Saline

000) as well as other antibody based fluorescent assays.

000)
Flow Cytometry/FACS (1/1

000-1/200

000-1/40

000.
Immunohistochemistry: 1/1

000.
Protein G Peroxidase is a useful reagent in Western Blotting and ELISA experiments. Protein G Peroxidase can be utilized as a pseudo-secondary detection reagent when used in conjunction with an IgG-based primary antibody and appropriate substrate (such as TMB-1000 or Femtomax-110).

000.
Western blot: 1/10

Biological activity/ Working concentration: Optimum concentration for human umbilical vein endothelial cells (HUVEC) range from 50-200 μg/ml

ELISA.
Western Blot.
Cell culture and/or animal studies.

ELISA.
Western blotting.

Functional Assays.

Positive control for Western blot analysis

Positive control for Western blot analysis.
Standard for ELISA.

Adermann K. Role of the prosequence of guanylin. Protein Sci. 1999 Sep; 8(9): 1850-9.
4. Lauber T

Cell culture and/or animal studies.
ELISA.
Western Blot.

Cohen MB. Proguanylin secretion and the role of negative-feedback inhibition in a villous epithelial cell line. Am J Physiol Gastrointest Liver Physiol. 2002 Sep; 283(3): G695-702.
3. Schulz A

ELISA.
Functional Assays.

ELISA.
Western blotting.

Forssmann WG

Hawkins JA

Hidaka Y

Immunofluorescence Microscopy (1/1

Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-biotin-avidin based enzymatic assays.
Recommended Dilutions:
ELISA: 1/20

Marx UC

Marx UC. Native and recombinant proguanylin feature identical biophysical properties and are monomeric in solution. Biochemistry. 2002 Dec 10; 41(49): 14602-12.
2. Rudolph JA

Marx UC. Solution structure of human proguanylin: the role of a hormone prosequence. J Biol Chem. 2003 Jun 27; 278(26): 24118-24. Epub 2003 Apr 21.

Neudecker P

Nourse A

Rosch P

Schulz A

Shimonishi Y

Suitable for Immunoblotting (Western or dot blot)

Western Blot.
ELISA.

Western Blot.
Protocol: 1. Lauber T

Western Blotting.

optimal concentration with heparin (30 μg/ml) is about 12 μg/ml.

MAPK signaling pathway

Pathways in cancer

Focal adhesion

ErbB signaling pathway

Neurotrophin signaling pathway

Endocytosis

GnRH signaling pathway

Prostate cancer

Regulation of actin cytoskeleton

Chemokine signaling pathway

Colorectal cancer

Progesterone-mediated oocyte maturation

Pancreatic cancer

Cytokine-cytokine receptor interaction

Glioma

Insulin signaling pathway

Fc epsilon RI signaling pathway

Calcium signaling pathway

Melanoma

Adherens junction

T cell receptor signaling pathway

Axon guidance

Toll-like receptor signaling pathway

Non-small cell lung cancer

Oocyte meiosis

Wnt signaling pathway

Long-term potentiation

VEGF signaling pathway

Epithelial cell signaling in Helicobacter pylori infection

Gap junction

Endometrial cancer

Bladder cancer

Chronic myeloid leukemia

Vascular smooth muscle contraction

Acute myeloid leukemia

Fc gamma R-mediated phagocytosis

Renal cell carcinoma

TGF-beta signaling pathway

Melanogenesis

Tight junction

Adipocytokine signaling pathway

mTOR signaling pathway

Natural killer cell mediated cytotoxicity

B cell receptor signaling pathway

NOD-like receptor signaling pathway

Long-term depression

Type II diabetes mellitus

RIG-I-like receptor signaling pathway

Apoptosis

Cell cycle

Olfactory transduction

Leukocyte transendothelial migration

Small cell lung cancer

Hedgehog signaling pathway

Prion diseases

Thyroid cancer

Jak-STAT signaling pathway

Dorso-ventral axis formation

Amyotrophic lateral sclerosis (ALS)

p53 signaling pathway

Alzheimer's disease

Vibrio cholerae infection

Hematopoietic cell lineage

Viral myocarditis

Cytosolic DNA-sensing pathway

Pathogenic Escherichia coli infection

Dilated cardiomyopathy

Primary immunodeficiency

Regulation of autophagy

Taste transduction

Phosphatidylinositol signaling system

Circadian rhythm - mammal

PPAR signaling pathway

Purine metabolism

Ubiquitin mediated proteolysis

Basal cell carcinoma

Hypertrophic cardiomyopathy (HCM)

Nucleotide excision repair

Parkinson's disease

Human

E. coli

Human 293

1

2

Yes

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