FITC Conjugated Goat Anti-Mouse IgG
| Applications | FC: 1:100; IF/ICC: 1:32-64; IHC: 1:32-64 |
| Reactivities | Mouse IgG |
| Conjugation | FITC |
Secondary Antibodies
Monkey IgG (Fab specific) rabbit polyclonal antibody, HRP
| Applications | Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommneded Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/10000. |
| Reactivities | Monkey |
| Conjugation | HRP |
Biotin Conjugated Goat Anti-Mouse IgG
| Applications | WB: 1:200-400; ICC: 1:100-200; IHC: 1:100-200; ELISA: 1:5,000-20,000 |
| Reactivities | Mouse IgG |
| Conjugation | Biotin |
Porcine IgG (Fc specific) rabbit polyclonal antibody, HRP
| Applications | ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry Paraffin Sections. Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of swine origin known to be of the IgG isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgG in swine serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemistry and Cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000. |
| Reactivities | Porcine |
| Conjugation | HRP |
Mouse IgA (alpha chain specific) goat polyclonal antibody, Biotin
| Applications | ELISA: 1/5,000-1/20,000. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, PE
| Applications | Flow Cytometry: (< / = 0.1 µg/10e6 cells) and Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells. FLISA: < / = 1.0 µg/ml |
| Reactivities | Rat |
| Conjugation | PE |
Mouse IgE (Fc specific) goat polyclonal antibody, HRP
| Applications | In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000. depending on the method used. |
| Reactivities | Mouse |
| Conjugation | HRP |
Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Purified
| Applications | ELISA (both capture and detection). Western Blot. Radioimmunoassay |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgG (gamma chain specific) rabbit polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western Blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000. |
| Reactivities | Human |
| Conjugation | Unconjugated |
IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified
| Applications | ELISA: 1/1000-1/2000 (2-9). Immunohistochemistry on Frozen Sections: reported in literature (5,7,10,11). Immunohistochemistry on Paraffin Sections: reported in literature (10). Flow Cytometry: reported in literature (15, 18, 19). Immunocytochemistry: reported in literature (12). Western Blot: reported in literature (3,13-16). |
| Reactivities | Human |
| Conjugation | AP |
Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified
| Applications | Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C. SDS-PAGE (12% separating gel). Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min. Strongly reacts with Bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry on Frozen Sections. |
| Reactivities | Bovine, Sheep |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain) sheep polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/200,000 Western blot: 1/1,000-1/5,000. Immunohistochemistry: 1/500-1/2,500. |
| Reactivities | Mouse |
| Conjugation | HRP |
Human IgG2 (Hinge Region) mouse monoclonal antibody, clone 3C7, Purified
| Applications | ELISA. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Guinea Pig IgM (Fc specific) goat polyclonal antibody, Biotin
| Applications | In immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure; in non-isotopic assay methodology (e.g. ELISA) to measure IgM in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: Histochemical and Cytochemical: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/1000 - 1/5000. |
| Reactivities | Guinea Pig |
| Conjugation | Biotin |
Monkey IgG (Fab specific) rabbit polyclonal antibody, FITC
| Applications | Direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. |
| Reactivities | Monkey |
| Conjugation | FITC |
优化搜索结果
Reactivities
- Mouse (494)
- Human (432)
- Rat (196)
- Rabbit (150)
- Goat (80)
- Chicken (73)
- Monkey (69)
- Sheep (65)
- Bovine (45)
- Guinea Pig (45)
- Hamster (39)
- Canine (34)
- Feline (30)
- Porcine (28)
- Horse (21)
- Dog (18)
- Pig (14)
- Duck (11)
- Equine (9)
- Donkey (7)
- Golden Syrian Hamster (7)
- Turkey (6)
- Chimpanzee (4)
- Mouse IgG (3)
- Human IgG (2)
- Reptiles (2)
- Goat IgG (1)
- Human Ig light chain (1)
- Human IgA (1)
- Human IgA1 (1)
- Human IgA2 (1)
- Human IgD (1)
- Human IgE (1)
- Human IgG1 (1)
- Human IgG3 (1)
- Human IgM (1)
- Human gamma heavy chain (1)
- Human kappa light chain (1)
- Human lambda light chain (1)
- Mammalian (1)
- Mouse Ig kappa light chain (1)
- Mouse IgG gamma (1)
- Mouse IgG1 (1)
- Mouse IgG2a kappa (1)
- Mouse IgG2b (1)
- Mouse IgM lambda light chain (1)
- Mouse IgM mu-chain (1)
- Rabbit IgG (1)
- Rabbit IgG Fab (1)
- Rabbit IgG Fc (1)
Applications
Antibody Hosts
Conjugates
- Unconjugated (712)
- FITC (453)
- HRP (447)
- Biotin (331)
- TRITC (243)
- Texas Red (206)
- AP (150)
- Alkaline Phosphatase (87)
- PE (60)
- Phycoerythrin (21)
- ATTO 647N (15)
- ATTO 425 (14)
- ATTO 532 (14)
- ATTO 550 (14)
- ATTO 594 (14)
- ATTO 488 (13)
- ATTO 655 (13)
- Cy5 (6)
- APC (5)
- Cy3 (4)
- Agarose (3)
- BGAL (1)
- BIMA (1)
- PE-Cy7 (1)
