Secondary Antibodies

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Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]
AM20252BT-N

CNY 8,794.00

5周

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), Biotin

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used.
General Recommended Dilutions:
Immunocytochemistry.
Immunohistochemistry (Paraffin).
Histochemical Use: 1/10-1/40.
ELISA: 1/100-1/2000. 
Western blot: 1/200-1/4000.
Reactivities Human
Conjugation Biotin
IGHG4 Mouse Monoclonal Antibody [Clone ID: HP6025]
SM2069AP

CNY 4,323.00

4周

IGHG4 mouse monoclonal antibody, clone HP6025, AP, Purified

Applications ELISA: 1/1000-1/2000 (2-9).
Immunohistochemistry on Frozen Sections:  reported in literature (5,7,10,11).
Immunohistochemistry on Paraffin Sections: reported in literature (10).
Flow Cytometry: reported in literature (15, 18, 19).
Immunocytochemistry: reported in literature (12).
Western Blot: reported in literature (3,13-16).
Reactivities Human
Conjugation AP
Human IgA Mouse Monoclonal Antibody [Clone ID: NI 69 (A89-034) and NI 184 (A89-035)]
AM20249AF-N

CNY 5,902.00

5周

Human IgA mouse monoclonal antibody, clone NI 69 (A89-034) and NI 184 (A89-035), Ascites

Applications To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200. 
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated
Human IgG2 (Fc specific) Mouse Monoclonal Antibody [Clone ID: 31-7-4]
AM08152HR-N

CNY 4,169.00

4周

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, HRP

Applications ELISA: 1/2,000-1/8,000.
Reactivities Human
Conjugation HRP
Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]
AM20252HR-N

CNY 8,794.00

5周

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), HRP

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
ELISA: 1/20-1/500.
Western Blot: 1/40-1/1000. 
Histochemical Use: 1/5-1/20.
Reactivities Human
Conjugation HRP
Rat IgA (heavy chain) Mouse Monoclonal Antibody [Clone ID: MARA-1]
SM1027P

CNY 5,951.00

4周

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, Purified

Applications ELISA: 5 µg/ml. This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 (Cat.-No AM05512HR-N) as detection reagent and purified Rat IgA (Cat.-No SA013) as antigen.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Reactivities Rat
Conjugation Unconjugated
Human IgE Mouse Monoclonal Antibody [Clone ID: BE5]
SM3061P

CNY 2,750.00

5周

Human IgE mouse monoclonal antibody, clone BE5, Purified

Applications ELISA.
Flow Cytometry: 5-10 µg/ml.
Reactivities Human
Conjugation Unconjugated
Human IgA (secretory) Mouse Monoclonal Antibody [Clone ID: SC-05]
SM3073P

CNY 2,880.00

5周

Human IgA (secretory) mouse monoclonal antibody, clone SC-05, Purified

Applications Western blot.
Immunoprecipitation.
Immunohistochemistry on Paraffin Sections.
Immunohistochemistry on Frozen Sections.
Examples of positive Human tissues: Secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.
Reactivities Human
Conjugation Unconjugated
Bovine IgG (light chain) Mouse Monoclonal Antibody [Clone ID: IVA285-1]
AM03077PU-N

CNY 3,140.00

5周

Bovine IgG (light chain) mouse monoclonal antibody, clone IVA285-1, Purified

Applications Western Blotting (Reducing Conditions): 0.5-1 μg/ml, overnight in 4°C.
SDS-PAGE (12% separating gel).
Positive Control and Sample Peparation: BS (Bovine Serum), dilution 1/50 in Laemmli reducing buffer, boiled in water bath for 3 min.
Strongly reacts with Bovine IgG light chains, weakly reacts with IgM.
Flow Cytometry.
Immunohistochemistry on Frozen Sections.
Reactivities Bovine, Sheep
Conjugation Unconjugated
Human IgE Mouse Monoclonal Antibody [Clone ID: BE5]
SM3061PE

CNY 3,940.00

5周

Human IgE mouse monoclonal antibody, clone BE5, PE

Applications Flow cytometry: 5 µg/ml.
Reactivities Human
Conjugation PE
Human IgE Mouse Monoclonal Antibody [Clone ID: BE5]
SM3061F

CNY 3,410.00

5周

Human IgE mouse monoclonal antibody, clone BE5, FITC

Applications Flow cytometry: 5 µg/ml.
Reactivities Human
Conjugation FITC
Human IgG3 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 330 (HP 6050)]
AM20255HR-N

CNY 8,794.00

5周

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), HRP

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation HRP
Human IgG (Isotype Specific) Mouse Monoclonal Antibody [Clone ID: NI 335 and NI 343]
AM20258AF-N

CNY 5,902.00

5周

Human IgG (Isotype Specific) mouse monoclonal antibody, clone NI 335 and NI 343, Ascites

Applications To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation Unconjugated