Secondary Antibodies

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Human IgG4 (pFC) Mouse Monoclonal Antibody [Clone ID: HP6023 (also known as HO6023)]
AM01158HR-N

CNY 3,487.00

4周

Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), HRP, Purified

Applications ELISA: 1/4000-1/8000 (See also Ref.2-9).
Applications Reported in Literature:
Flow Cytometry (Ref.15,18,19).
Immunohistochemistry on Frozen Sections (Ref.10).
Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11).
Immunocytochemistry (Ref.12).
Immunoblotting (Ref.3,13-16).
Microarray (Ref.17).
Multiplex (Ref.2,20-22).
Purification (Ref.15).
Reactivities Human
Conjugation HRP
Rabbit IgG (H+L chain) Goat Polyclonal Antibody
R1364HRP

CNY 2,741.00

4周

Rabbit IgG (H+L chain) goat polyclonal antibody, HRP, Lyophilized

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/150,000-1/250,000.
Immunohistochemistry: 1/500-1/2,500.
Western blot: 1/10,000-1/40,000.
Reactivities Rabbit
Conjugation HRP
Human IgG2 (Fc specific) Mouse Monoclonal Antibody [Clone ID: 31-7-4]
AM08152HR-N

CNY 4,169.00

4周

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, HRP

Applications ELISA: 1/2,000-1/8,000.
Reactivities Human
Conjugation HRP
Human IgG1 (Fc specific) Mouse Monoclonal Antibody [Clone ID: NI 132 (HP 6186)]
AM20252HR-N

CNY 8,794.00

5周

Human IgG1 (Fc specific) mouse monoclonal antibody, clone NI 132 (HP 6186), HRP

Applications To identify the presence of IgG1 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG1, and Immunoblotting.
General Recommended Dilutions:
ELISA: 1/20-1/500.
Western Blot: 1/40-1/1000. 
Histochemical Use: 1/5-1/20.
Reactivities Human
Conjugation HRP
Mouse IgG (H+L chain) Rabbit Polyclonal Antibody
R1253HRP

CNY 2,919.00

4周

Mouse IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA : 1:50,000 - 1:300,000
Western Blot : 1:10,000 - 1:30,000
Immunohistochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Reactivities Mouse
Conjugation HRP
Mouse IgG (H+L chain) Sheep Polyclonal Antibody
R1256HRP

CNY 2,877.00

4周

Mouse IgG (H+L chain) sheep polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/10,000-1/200,000
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Mouse
Conjugation HRP
Goat IgG (H+L chain) Rabbit Polyclonal Antibody
R1317HRP

CNY 2,963.00

4周

Goat IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications ELISA: 1/50,000-1/100,000. 
Western Blot: 1/5,000-1/20,000. 
Immunohistochemistry: 1/1,000-1/5,000. 
Note: HRP Secondary antibody reagents are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Reactivities Goat
Conjugation HRP
Rabbit IgG (H+L chain, adsorbed) Goat Polyclonal Antibody
R1454HRP

CNY 4,650.00

4周

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000. 
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/40,000 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation HRP
Mouse IgG (heavy chain) Rat Monoclonal Antibody [Clone ID: LO-MG-7]
AM32194HR-N

CNY 23,256.00

5周

Mouse IgG (heavy chain) rat monoclonal antibody, clone LO-MG-7, HRP, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on Frozen Sections. 
Reactivities Mouse
Conjugation HRP
Mouse IgE (Fc specific) Goat Polyclonal Antibody
AP21482HR-N

CNY 7,161.00

5周

Mouse IgE (Fc specific) goat polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
depending on the method used.
Reactivities Mouse
Conjugation HRP
Human IgA + IgG + IgM Goat Polyclonal Antibody
R1343HRP

CNY 3,938.00

4周

Human IgA + IgG + IgM goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunoprecipitation: 1/1,000-1/5,000.
Reactivities Human
Conjugation HRP
Human IgG3 (F(ab)2 specific) Mouse Monoclonal Antibody [Clone ID: NI 330 (HP 6050)]
AM20255HR-N

CNY 8,794.00

5周

Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), HRP

Applications To identify the presence of IgG3 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/500 upwards.
Western blot: from 1/1000 upwards.
Reactivities Human
Conjugation HRP
Rabbit IgA+IgG+IgM (H+L chain) Goat Polyclonal Antibody
AP21490HR-N

CNY 7,161.00

5周

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids).
Immunocytochemistry.
Immunohistochemistry.
Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties.
Dot blot.
Western blot.
General Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen.
Reactivities Rabbit
Conjugation HRP
Mouse IgG (Fc specific) Goat Polyclonal Antibody
R1612HRP

CNY 3,544.00

4周

Mouse IgG (Fc specific) goat polyclonal antibody, HRP

Applications This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000
Western blot: 1/1,000-1/5,000
Flow Cytometry: 1/500-1/2,500
Reactivities Mouse
Conjugation HRP