Secondary Antibodies

Guinea Pig IgG (Fc specific) sheep polyclonal antibody, FITC

Applications ELISA.
(In)direct immunofluorescence.

Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Can be  used in direct staining of cytoplasmic IgG in fixed guinea pig cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of guinea pig in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Guinea Pig
Conjugation FITC

Sheep IgG (Fc specific) rabbit polyclonal antibody, FITC

Applications ELISA. 
Immunocytochemistry.  
(In)direct Immunofluorescence.

Immunohistochemistry on Frozen Sections.
Can be used In direct staining of cytoplasmic IgG in fixed sheep cells and tissue substrates; to identify circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of sheep in the middle layer of the test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions1/20-1/80.
Reactivities Sheep
Conjugation FITC

Duck IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
(In)direct Immunofluorescence.

Immunohistochemistry on Frozen Sections.
Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of duck origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:  1/20-1/80.
Reactivities Duck
Conjugation FITC

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, FITC

Applications

FLISA.
Immunofluoresecence: ≤ 1 μg/106 cells.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation FITC

Human IgG3 (Hinge Region) mouse monoclonal antibody, clone HP6050, FITC

Applications FLISA: 1/200-1/400
Immunocytochemistry.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, FITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
Flow cytometry 1:2,000 - 1:10,000
Immunofluorescence 1:1,000 - 1:5,000
FLISA:1:10,000-1:50,000.
Reactivities Rabbit
Conjugation FITC

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, FITC

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation FITC

Mouse IgG3 (heavy chain) rat monoclonal antibody, clone LO-MG3-7, FITC, Purified

Applications

Useful as second antibody in Immunoassays, such as ELISA's (good binding on plastic) and Immunohistochemistry on Frozen Sections.
The antibody can be used at 5 μg/ml.

Reactivities Mouse
Conjugation FITC

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, FITC

Applications This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Immunofluorescence: 1/1,000-1/5,000.
Flow Cytometry: 1/500-1/2,500.
Reactivities Mouse
Conjugation FITC

Mouse IgG (Fc specific), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.  
Recommended Dilutions:
FLISA: 1/10,000-1/50,000. 
IF Microscopy: 1/1,000-1/5,000. 
Flow Cytometry: 1/500-1/2,500. 

 
Reactivities Mouse
Conjugation FITC

Mouse IgE (heavy chain) rat monoclonal antibody, clone LO-ME-3, FITC

Applications Useful as second antibody in Immunoassays, such as ELISA's
Reactivities Mouse
Conjugation FITC