TMIGD2 (23-150, hIgG-Tag) Human Recombinant Protein

Specifications

Product Data
Species	Human
Expression Host	HEK293
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) LSVQQGPNLL QVRQGSQATL VCQVDQATAW ERLRVKWTKD GAILCQPYIT NGSLSLGVCG PQGRLSWQAP SHLTLQLDPV SLNHSGAYVC WAAVEIPELE EAEGNITRLF VDPDDPTQNR NRIASFPG
Tag	hIgG-Tag
Predicted MW	40.1kDa (361aa)
Concentration	1mg/ml (determined by Absorbance at 280nm)
Purity	95% by SDS - PAGE
Buffer	Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Applications	SDS-PAGE, Bioactivity
Storage	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Bioactivity	Measured by its binding ability in a functional ELISA with Human HHLA2. The ED50 range ≤ 200 ng/ml.
Endotoxin	<1 EU per 1ug of protein (determined by LAL method)
Reference Data
RefSeq	NP_653216.2
Summary	TMIGD2, also known as IGPR-1, is a member of the Immunoglobulin family. This protein shares approximately 10% amino acid sequence identity with CD28, CTLA­4, ICOS, and PD­1. The immunoglobulin domain of TMIGD2 was predicted to be Ig V fold and was found to be highly similar to the Ig domain of myelin-associated glycoprotein. It plays a role in cell-cell interaction, cell migration, and angiogenesis. Through interaction with HHLA2, co-stimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade. It is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of TMIGD2 with B7­H7 activates the Akt­dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells. Also, it interacts with multiple cytoskeletal proteins. Recombinant human TMIGD2, fused to hIgG-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

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