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PRAME (NM_206955) Human Recombinant Protein
CAT＃: TP307874
Purified recombinant protein of Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 4, 20 µg
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Specifications
| Product Data | |
| Species | Human |
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence |
>RC207874 protein sequence
Red=Cloning site Green=Tags(s) MERRRLRGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAALELLPRELFPPLFMAAFDGRHSQTLK AMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNR ASLYSFPEPEAAQPMTKKRKVDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCC KKLKIFAMPMQDIKMILKMVQLDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIHASSYISPEK EEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQLLRHVMNPLETLSITNCRLSEGDVMHLSQSPSVSQ LSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGITDDQLLALLPSLSHCSQLTTLSFYGNSISI SALQSLLQHLIGLSNLTHVLYPVPLESYEDIHGTLHLERLAYLHARLRELLCELGRPSMVWLSANPCPHC GDRTFYDPEPILCPCFMPN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Tag | C-Myc/DDK |
| Predicted MW | 57.7 kDa |
| Concentration | >0.05 µg/µL as determined by microplate BCA method |
| Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol |
| Preparation | Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| Note | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
| Storage | Store at -80°C. |
| Stability | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
| Reference Data | |
| RefSeq | NP_996838 |
| Locus ID | 23532 |
| UniProt ID | P78395 |
| Refseq Size | 2435 |
| Cytogenetics | 22q11.22 |
| Refseq ORF | 1527 |
| Synonyms | CT130; MAPE; OIP-4; OIP4 |
| Summary | This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| LC401847 | PRAME HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC404191 | PRAME HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC404192 | PRAME HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 1,470.00 |
|
| LC404193 | PRAME HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LC404194 | PRAME HEK293T cell transient overexpression lysate (as WB positive control) |
CNY 900.00 |
|
| LY401847 | Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 1 |
CNY 3,080.00 |
|
| LY404191 | Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 2 |
CNY 3,080.00 |
|
| LY404192 | Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 3 |
CNY 4,840.00 |
|
| LY404193 | Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 4 |
CNY 3,080.00 |
|
| LY404194 | Transient overexpression lysate of preferentially expressed antigen in melanoma (PRAME), transcript variant 5 |
CNY 3,080.00 |
|
| PH307874 | PRAME MS Standard C13 and N15-labeled recombinant protein (NP_996838) |
CNY 14,250.00 |
|
| TP760464 | Purified recombinant protein of Human preferentially expressed antigen in melanoma (PRAME), transcript variant 3, with N-terminal HIS tag, expressed in E.Coli, 50ug |
CNY 2,040.00 |
|
| TP760913 | Purified recombinant protein of Human preferentially expressed antigen in melanoma (PRAME), transcript variant 5, full length, with N-terminal HIS tag, expressed in E. coli, 50ug |
CNY 2,040.00 |
