NEDD8 (1-76, His-tag) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMLI KVKTLTGKEI EIDIEPTDKV ERIKERVEEK EGIPPQQQRL IYSGKQMNDE KTAADYKILG GSVLHLVLAL RGG
Tag	His-tag
Predicted MW	12.8 kDa
Concentration	lot specific
Purity	>95% by SDS - PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.5 mM DTT, 50 mM NaCl, 10% glycerol
Preparation	Liquid purified protein
Protein Description	Recombinant human NEDD8 protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Endotoxin	< 1.0 EU per 1 μg of protein (determined by LAL method)
Reference Data
RefSeq	NP_006147
Locus ID	4738
UniProt ID	Q15843
Cytogenetics	14q12
Synonyms	NEDD-8
Summary	Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.[UniProtKB/Swiss-Prot Function]
Protein Families	Druggable Genome

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