Fumarase (44-510) Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Expression cDNA Clone or AA Sequence	Protein Sequence (showhide) MASQNSFRIE YDTFGELKVP NDKYYGAQTV RSTMNFKIGG VTERMPTPVI KAFGILKRAA AEVNQDYGLD PKIANAIMKA ADEVAEGKLN DHFPLVVWQT GSGTQTNMNV NEVISNRAIE MLGGELGSKI PVHPNDHVNK SQSSNDTFPT AMHIAAAIEV HEVLLPGLQK LHDALDAKSK EFAQIIKIGR THTQDAVPLT LGQEFSGYVQ QVKYAMTRIK AAMPRIYELA AGGTAVGTGL NTRIGFAEKV AAKVAALTGL PFVTAPNKFE ALAAHDALVE LSGAMNTTAC SLMKIANDIR FLGSGPRSGL GELILPENEP GSSIMPGKVN PTQCEAMTMV AAQVMGNHVA VTVGGSNGHF ELNVFKPMMI KNVLHSARLL GDASVSFTEN CVVGIQANTE RINKLMNESL MLVTALNPHI GYDKAAKIAK TAHKNGSTLK ETAIELGYLT AEQFDEWVKP KDMLGPK
Predicted MW	50.2 kDa
Concentration	lot specific
Purity	>95% by SDS - PAGE
Buffer	Presentation State: Purified State: Liquid purified protein Buffer System: 20 mM Tris-HCl buffer (pH 8.0)
Preparation	Liquid purified protein
Applications	Protocol: Activity Assay 1. Prepare 1.45 ml assay buffer (Assay buffer: 100 mM potassium phosphate, 50 mM L-malic acid). 2. Add 50 ul of recombinant Fumarase protein with various concentrations (0.5 ug, 1ug, 2ug) in assay buffer. 3. Record the increase in A260 nm for 10 minutes - L-Malate (Sigma-Aldrich. Cat. No, M-1000) - 96 well UV plate (Costar, Cat. No, 3635) - plate reader (PerkinElmer, VICTORX3)
Protein Description	Recombinant Fumarase was expressed in E.coli and was purified by conventional chromatography techniques.
Storage	Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Bioactivity	Specific: > 1.0 unit/mg, defined as the amount of enzyme that cleaves 1 umole of L-Malate to Fumarate per minute at pH 7.5 at 25°C
Endotoxin	< 1.0 EU per 1 μg of protein (determined by LAL method)
Reference Data
RefSeq	NP_000134
Locus ID	2271
UniProt ID	P07954, A0A0S2Z4C3
Cytogenetics	1q43
Synonyms	FMRD; HLRCC; HsFH; LRCC; MCL; MCUL1
Summary	The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]
Protein Families	Druggable Genome
Protein Pathways	Citrate cycle (TCA cycle), Metabolic pathways, Pathways in cancer, Renal cell carcinoma

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.