GPAM (NM_020918.1) Human Over-expression Lysate

Specifications

Product Data
Description	Transient overexpression lysate of human glycerol-3-phosphate acyltransferase, mitochondrial (GPAM)
Species	Human
Expression Host	HEK293T
Expression cDNA Clone or AA Sequence	TrueORF Clone RC205509
Tag	C-MYC/DDK
Predicted MW	93.9 kDa
Components	1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
Storage	The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Avoid repeated freeze-thaw cycles. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Preparation	HEK293T cells in 10-cm dishes were transiently transfected with PEI and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 72hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -80°C before shipping.
Reference Data
Locus ID	57678
Cytogenetics	10q25.2
Protein Pathways	Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways

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