CNOT7 Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
Donor DNA	RFP-BSD
Symbol	CNOT7
Locus ID	29883
Kit Components	KN209293G1, CNOT7 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: TAAAACGCTTCATATAAAGG KN209293G2, CNOT7 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGTCTTGAATTCAACCCTTT KN209293RB-D, donor DNA containing left and right homologous arms and RFP-BSD functional cassette. Homologous arm and RFP-BSD sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet AGAGCATTTA CAAACATTTT TGACCCCTCT AGTCTAGTTA GAATAAACGC CGGTAAAGTA GATCTTGTTA TCAAGTCGGA TTTTGGTTGT TTTATTAATG AGGAATAAAA ATAAACACCA TCTTTGCCAT GGTAACATCT GATCTTGTCA CATGTAAATA AGTAATTTTT CATTTTTTGT CTGGAAATGG ATGGCTTTAT AAAGTTTTAT GCAGAGTAGT TGAACTCAGA TAAATAATTA GTGGAAAGTT ATAAAATATG ATTAGTATGT ATGTGTAAAA AAGCTATTTT AAAATAACTG AAATTAGGAC CAGGAAATCT GTACTGTAGT CCTTTTTTTC CTATAGACTA GCTGTGTGAC CTTAAGTTAC TTTCTAAACT TTGATTTTTT TTTTTTATGA GTCAAGAACT TTGAATAAAT AACTTCTTAG GTTCCTGCTG GATCAATTAT TCTGTGATTC AGATTTGATA TATCTGGTCT TCATATTGTT TTTTTATTTT GACAGGTACA TAAATAAAGG ATAAAATATT TTATGAAACA AATCTTCAAT CAAGTATAAC ATTTTGATGC TTGGCATCTA GACTCCCTTG TGCCCTCACT GGATTAATTT CACCATGAAA AGTAAAATTT GTCACTGCCC TCCAGCCAAA ATCCATCAGG AATACACCTG TGATTTGACA GGTTGGGTTT ATCCCTCATT GCAGTGAGGG AAAACGCACT GGAATTCATG GGAACCATGG GCCGTTTGAA TAAGAGTTAG GAAGGATTTG GGCTCGTGTC AGGTGGTCTT AGGGGAAGGT AAGGGAAGCA GAGCTTTACT GTGGATTGGA TGTGTTCGGG AAGCTGGGGT AATTCTGTGA TTGGGTATCT TACTAAGTCT TAAATGAGAG ACTAGAATGA GGTTAAAGCT GCAGAGAAGG AACAGTCATT CATTTAGCCA GGATGGAGAT ACTTGGTCAT TTTGTGGTTT GACATTTTTA TTTTTTTGTC CTTGTTCAGA CATGATTACA GAGTGGCCTT ATTTGAGGTT GAACTTTTGT GAAATTGTGT TCAACAGGAG AACATTAGGG CGTGGCTATG AGTGCCAGGT CTGCTCCTAG CAACACCAAA GCCTAGCTGG TAGTACCACA TGAGCTCCCA GAAGCTAGGG TGCTCTTTCT CAAACAAAAA AATCAGATGT GCCAGTGAAA CGTTGTTGGT GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_013354, NM_054026, NM_001322087, NM_001322088, NM_001322089, NM_001322090, NM_001322091, NM_001322092, NM_001322093, NM_001322094, NM_001322095, NM_001322096, NM_001322097, NM_001322098, NM_001322099, NM_001322100
Synonyms	CAF1; Caf1a; hCAF-1
Summary	The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The encoded protein downregulates the innate immune response and therefore provides a therapeutic target for enhancing its antimicrobial activity against foreign agents. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and X. [provided by RefSeq, Apr 2016]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.