anchored capsid protein C (NC_001474) Virus Tagged ORF Clone

CAT#: VC102541

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for anchored capsid protein C [Dengue virus 2], codon optimized for human cell expression, NP_739581



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CNY 3,800.00


货期*
3周

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Synonyms DENV_gp1, Dengue, DENV
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC102541 represents NCBI reference of NP_739581 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATAATCAACGAAAAAAAGCAAAGAATACGCCTTTCAATATGCTTAAAAGGGAACGGAACAGGGTAT
CAACAGTACAGCAACTCACAAAGCGGTTTAGTCTGGGAATGTTGCAGGGAAGAGGGCCCCTCAAGCTCTT
CATGGCACTGGTAGCATTCTTGCGGTTTCTCACCATCCCACCAACAGCCGGAATACTTAAACGATGGGGC
ACAATTAAAAAATCCAAAGCTATAAACGTACTCAGAGGTTTTAGAAAAGAGATCGGTCGAATGCTCAATA
TACTTAATAGAAGAAGAAGATCAGCAGGCATGATCATTATGCTTATTCCAACAGTCATGGCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC102541 representing NP_739581
Red=Cloning sites Green=Tags

MNNQRKKAKNTPFNMLKRERNRVSTVQQLTKRFSLGMLQGRGPLKLFMALVAFLRFLTIPPTAGILKRWG
TIKKSKAINVLRGFRKEIGRMLNILNRRRRSAGMIIMLIPTVMA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001474
ORF Size 342 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NC_001474.2, NP_739581
RefSeq ORF 342 bp
MW 13.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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