E4 (NC_001526) Virus Tagged ORF Clone

CAT#: VC101900

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV16gp5, partial [Human papillomavirus type 16], codon optimized for human cell expression, NP_041329



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CNY 1,320.00

CNY 3,800.00


货期*
现货

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101900 represents NCBI reference of NP_041329 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

TATTACGTGTTGCATCTCTGTCTGGCTGCTACGAAGTACCCTCTGCTGAAACTGCTTGGGAGCACCTGGC
CAACAACTCCACCCCGCCCTATCCCTAAGCCTTCTCCATGGGCACCCAAAAAACACAGGAGGCTGAGTAG
TGACCAGGACCAGTCCCAGACACCAGAAACCCCCGCAACACCACTTTCATGCTGCACCGAGACTCAGTGG
ACAGTACTCCAGAGCTCCCTGCATCTCACGGCCCATACAAAGGATGGACTTACCGTCATCGTCACACTGC
ACCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101900 representing NP_041329
Red=Cloning sites Green=Tags

YYVLHLCLAATKYPLLKLLGSTWPTTPPRPIPKPSPWAPKKHRRLSSDQDQSQTPETPATPLSCCTETQW
TVLQSSLHLTAHTKDGLTVIVTLHP

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001526
ORF Size 285 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NC_001526.2, NP_041329
RefSeq ORF 285 bp
Locus ID 1489076
UniProt ID P03120
MW 10.6 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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