E2 (NC_012213) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC101847 represents NCBI reference of YP_002647036 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAACACTTGTTCAGCGGTTCGATGTCGTTCAGGACAAGCTCCTCGGATTGTATGAGGCCGGGCACA CCGACCTGGATTCTCAGATACAATACTGGGACCTTGTTAGACAGGAAAATGTGCTGCTGAACTTTGCGAG GAGACACGGTATCCAAATGTTGGGATTGCAGAGAGTCCCCCCCCTGGCTATCTCAGAGAGCAAGGCAAAG ACGGCCATTATGATGCGGCTTGTTCTTGACTCTCTTAAAAAGTCTCCATTCGGTCAGGAACCATGGGGGC TGACTGATACCTCACACGAGCTGTACACTACGCCTCCAGAGAACACATTTAAGAAAGGCGGCGCCCAGGT GGAGGTCCTGTACGACAACGAGGAAAACAAAGCAATGCCTTATACCAAATGGAAATATATCTACTACCAG GACTCAGAGGATCTGTGGCATAAGACGCACGCACAGGTTGACGCGAAAGGTCTGTACTTTGTAGACACCC AAATGCACAAGCATTACTACGTCCAGTTTGATAAAGAGGCCATGCAGTATGGGCACAAGGGAGTGTGGCA GGTGAGAACCGGCACCGACACGATTTTCGGCGCTTCTTCTGTTGCCAGTACATCACGCGGCCCAGACGCC ACGTCTACTGATCCTCAGGCAGGGCGCCTCCCAGAAAGAAAGAGGAAGAGACGGCGCCGCTACCCATCCA CGACGACTACTGCAACCAGCGAATCAGATACCGAGGAAAGCACTAGCGACAATCGCAGACGCAAGCGGAG ACGCCTGCGAGAGCGGAAGCACCGATCTCGCCATAGGTGGGGCACAGGGACTGAATCCGCTTCCGATACA AGCGCCAGCGTAGGCTCTCGACACCGATCACCGAACAGGCATTATCCCTCCCGCCTCGCTAGATTGCAGG CCGAGGCGCGGGACCCGCCTATACTCATCGTGAAGGGACCCTCCAATACGCTGAAATGTTGGAGGTACAG GTGCAAAACCAAATATTCTGGACTGTATGTTAACATATCTACAGGATTCACCTGGGTGACTGGGGCCGGG ACTGAGGGACACGCTCGGATCCTCGTCGCTTTCAAGGATAAGATGCAGAGAGACCTCTTCATGCAAACCG TTAAACTCCCTAAGGGCACCTCCTGTGCCTTGGGCAACCTGGAGAGTTTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC101847 representing YP_002647036 Red=Cloning sites Green=Tags METLVQRFDVVQDKLLGLYEAGHTDLDSQIQYWDLVRQENVLLNFARRHGIQMLGLQRVPPLAISESKAK TAIMMRLVLDSLKKSPFGQEPWGLTDTSHELYTTPPENTFKKGGAQVEVLYDNEENKAMPYTKWKYIYYQ DSEDLWHKTHAQVDAKGLYFVDTQMHKHYYVQFDKEAMQYGHKGVWQVRTGTDTIFGASSVASTSRGPDA TSTDPQAGRLPERKRKRRRRYPSTTTTATSESDTEESTSDNRRRKRRRLRERKHRSRHRWGTGTESASDT SASVGSRHRSPNRHYPSRLARLQAEARDPPILIVKGPSNTLKCWRYRCKTKYSGLYVNISTGFTWVTGAG TEGHARILVAFKDKMQRDLFMQTVKLPKGTSCALGNLESL TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_012213
ORF Size	1170 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NC_012213.1, YP_002647036
RefSeq ORF	1170 bp
Locus ID	7559096
MW	44.8 kDa

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