E6 (NC_001694) Virus Tagged ORF Clone

CAT#: VC101825

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for transforming protein E6 [Alphapapillomavirus 3], codon optimized for human cell expression, NP_043444



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CNY 3,800.00


货期*
4周

规格
    • 10 ug

Product images

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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC101825 represents NCBI reference of NP_043444 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACCATGTAATCCGACCAACATCTTTCTCTTGTGCAAAGATTATGAAGTCGATTTCGAGGACCTGA
GACTCACATGCGTGTTCTGCAAGAACGAACTGACCACGGAGGAACTGCTGGCCTTCGCCCTCAAGGAGTT
GTCCATAGTGTGGCGACACAACTGGCCTTTCGGGGTGTGCGCACCATGTCTGGCAAGAGAGGTCAAGGTT
CGCGAACTGCGGCACTGGGATCATAGCTGTTACGGTCCAACCGTGGAGCAAACGACCGGCAGGTCTCTTG
CCGAGTTGTACATTCGCTGCCATGCATGTAGCAAGCCCCTGAGTATACAGGAAAAGGAGCATCAGGTCCA
GGCATACATTCATTTCCACTACATCGCGGGACAGTGGACTGGCAGATGTTGCCAGTGTCGCGGCCCTTGC
ACCGCGCGGTGGCAACCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC101825 representing NP_043444
Red=Cloning sites Green=Tags

MGPCNPTNIFLLCKDYEVDFEDLRLTCVFCKNELTTEELLAFALKELSIVWRHNWPFGVCAPCLAREVKV
RELRHWDHSCYGPTVEQTTGRSLAELYIRCHACSKPLSIQEKEHQVQAYIHFHYIAGQWTGRCCQCRGPC
TARWQP

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001694
ORF Size 438 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NC_001694.1, NP_043444
RefSeq ORF 438 bp
Locus ID 1403312
MW 17.0 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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