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E1 (NC_012485) Virus Tagged ORF Clone
CAT＃: VC101777
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for E1 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756540
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CNY 7,410.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101777 represents NCBI reference of YP_002756540 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACAAGGGGATCAACAGCCCCAACTCTCTGGAAGGATTCTCTGGATGGTACGTAGTGGAAGAAG CTGAGTGCGAGGACAAGCTGACTGATCTTGAGAAGTTGTTCGATGAGAGCGATTGCTCCAATATTTCCAA CTTGATTGACGATGGTGATGTGACCGACCAAGGCAATAGTCTGGCCCTGTTCAATCAGCAATTGCTGGAA GATTGTGACCAGCAGCTGGTGGAGTTGAAGAGGAAGTACTTTAGCCCAGCAAAGGAAACTGATGTGGACC TTTCTCCTCAGTTGCAGTGCGTGAGTCTGTCCGCTAGTAGCAAGAACATTAAACGGCGGCTGTTTGTTGA TTCAGGCCTCGGGAATGAAGCAGAGGACAGTAACGAGTCAACCCAGGTGGCCGAAAGCGATTCCAATGGC GACAAAGCTGCGGAGCAAAGTCAATCTACCGGCGATACCGATCTCAATGCTAATGGAGGCATCGTCCAGG AGCTGCTTAAATCCCGGAACGCCACCATAACTTTTCTGGGCAAATTTAAGGAGACATTCGGCCTCTCTTA CAAGGACCTGACTAGACCCTTCAAAAGCCACAAAACGTGTTGTCACAGCTGGGTGGCCAGTATCATCGGA GTGCAAGATGATGTTTACGAGGCCAGTAAGACCCTGCTGCAGACACATTGCGACTTCTTTCAGGTGATTA ACTATAGTCTGGCAAGCTACGTTTTCGTTCTCTACCTCTTTGAGTTCAAAAATAGTAAAAATCGCGAGAC ACTCCTGAAGCTCTTGTGTCAGATTCTGTGCGTGAAGGATATACAAATCATGGCTGACCCGCCTAAACAC AAGAATATGGTGGTAGCTCTGTATTTTTATAAGCAGGCACTGTCCTCTACCTGTTACAAGTACGGCGATT TCCCAAATTGGTTGCTGAAGCAGACGGTGGTCCAGCACCAAAACGAGGCGGAAACTTTCATCCTCGCCAA GATGGTTCAGTACGCCTACGACCACGACCTGGTGGACGACTCAGAGATCGCCTACCAGTATGCTCTGCTC GCCGACGATGACCCCAACGCCGCTGCCTGGCTGAACTCTAATAATCAGGCTAAACACGTGAAAGATTGTG CCACAATGGTCCGACATTACAAAAAATACGAAATGAGAAAAATGTCCATGTCAGAGTGGATCTTTTATTG TTGCGATAAGGTGAAAGAAGAATCAGACTGGAAAGTGATCGCTAGGTTCCTGAAGTACCAAACGGTTAAC TTTGTGAGCTTCCTGACAGCACTCCGCGACATGTTCAAGGGCATTCCGAAAAAACAGTGTATAGTTATCA CCGGACCTCCCAATACCGGCAAATCTTATTTCTGTGTGAGCCTTGTCACGTTTCTGAGGGGCAGAGTGGT TTCTTTCATGAACCATAAGTCTCACTTTTGGCTTCAGCCCCTGGCCGAAGGAAAGGTGGGATTTCTGGAT GATGCGACCCATGCATGTTGGCAGTTCATTGACATACATATGAGAAACGGATTTGATGGGAATCCCGTTT GCCTTGATAGCAAACATAAGAATCCTACACAGATGCGACTTCCGGCTCTGTTTATAACTACGAATGTAGA GGTCGACAAGGAAGACCAGTACTATTATTTGAAGTCAAGGCTCCAGGTTTTCAACTTCCCTAATGTGATG CCCCTGGACGATCTGGGAAACCCTGTGTATGAAATTACTGATAGCAGTTGGAATTCTTTCTTCAGGCGCT TGGAAAAGCAGTTGGATATTTGCAGACCGACTGAAAGCAACGATGGCGACCCCGACAGGGCCTTCAGATG TTTTAGGGGTGAGTCAGCCTCTACTATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101777 representing YP_002756540
Red=Cloning sites Green=Tags MADKGINSPNSLEGFSGWYVVEEAECEDKLTDLEKLFDESDCSNISNLIDDGDVTDQGNSLALFNQQLLE DCDQQLVELKRKYFSPAKETDVDLSPQLQCVSLSASSKNIKRRLFVDSGLGNEAEDSNESTQVAESDSNG DKAAEQSQSTGDTDLNANGGIVQELLKSRNATITFLGKFKETFGLSYKDLTRPFKSHKTCCHSWVASIIG VQDDVYEASKTLLQTHCDFFQVINYSLASYVFVLYLFEFKNSKNRETLLKLLCQILCVKDIQIMADPPKH KNMVVALYFYKQALSSTCYKYGDFPNWLLKQTVVQHQNEAETFILAKMVQYAYDHDLVDDSEIAYQYALL ADDDPNAAAWLNSNNQAKHVKDCATMVRHYKKYEMRKMSMSEWIFYCCDKVKEESDWKVIARFLKYQTVN FVSFLTALRDMFKGIPKKQCIVITGPPNTGKSYFCVSLVTFLRGRVVSFMNHKSHFWLQPLAEGKVGFLD DATHACWQFIDIHMRNGFDGNPVCLDSKHKNPTQMRLPALFITTNVEVDKEDQYYYLKSRLQVFNFPNVM PLDDLGNPVYEITDSSWNSFFRRLEKQLDICRPTESNDGDPDRAFRCFRGESASTI TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_012485 |
| ORF Size | 1848 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_012485.1, YP_002756540 |
| RefSeq ORF | 1848 bp |
| Locus ID | 7701329 |
| MW | 70.4 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101775 | Myc-DDK-tagged ORF clone of viral ORF for E6 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756538 |
CNY 3,800.00 |
|
| VC101776 | Myc-DDK-tagged ORF clone of viral ORF for E7 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756539 |
CNY 3,800.00 |
|
| VC101778 | Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756541 |
CNY 4,940.00 |
|
| VC101779 | Myc-DDK-tagged ORF clone of viral ORF for E4 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756542 |
CNY 3,800.00 |
|
| VC101780 | Myc-DDK-tagged ORF clone of viral ORF for L2 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756543 |
CNY 6,370.00 |
|
| VC101781 | Myc-DDK-tagged ORF clone of viral ORF for L1 protein [Human papillomavirus 109], codon optimized for human cell expression, YP_002756544 |
CNY 6,270.00 |
