UL27 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100815

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for capsid scaffold protein [Human herpesvirus 1], codon optimized for human cell expression, NP_044629



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CNY 13,680.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100815 represents NCBI reference of NP_044629 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCAGGGAGCTCCAGCTCGCGGGAGAAGATGGTTCGTCGTGTGGGCTCTCTTGGGCCTCACACTTG
GTGTCCTGGTGGCTAGTGCCGCTCCATCATCTCCAGGGACTCCAGGTGTCGCCGCTGCAACTCAAGCTGC
GAATGGTGGTCCAGCAACCCCTGCCCCACCAGCACCCGGCGCCCCACCAACTGGAGACCCAAAGCCAAAG
AAAAATCGGAAGCCAAAGCCACCGAAGCCACCCCGCCCAGCGGGAGACAACGCAACCGTGGCTGCAGGCC
ACGCAACACTGCGAGAACACCTGCGGGATATTAAGGCCGAAAACACTGATGCGAATTTCTATGTCTGCCC
ACCACCGACTGGGGCCACAGTCGTACAATTCGAGCAACCAAGGCGCTGCCCCACTAGGCCCGAGGGCCAG
AATTACACTGAAGGGATCGCAGTCGTTTTTAAGGAAAATATCGCTCCGTATAAGTTCAAGGCCACGATGT
ATTATAAGGATGTGACCGTAAGCCAGGTCTGGTTCGGCCACAGGTACTCTCAATTCATGGGAATATTTGA
GGACAGAGCACCCGTTCCGTTTGAAGAGGTGATCGACAAAATCAACGCTAAAGGAGTCTGTCGCAGTACC
GCAAAGTACGTCAGGAACAATCTTGAAACCACTGCCTTCCATCGGGACGATCATGAGACTGACATGGAGC
TCAAGCCAGCTAACGCTGCCACCAGGACCAGCAGGGGGTGGCACACTACAGACCTGAAGTATAACCCCTC
ACGGGTGGAAGCCTTCCACAGGTACGGGACGACCGTGAACTGTATTGTTGAAGAGGTCGACGCCAGGAGC
GTGTATCCTTATGATGAGTTCGTATTGGCAACGGGTGACTTCGTGTACATGAGCCCTTTCTACGGATACC
GCGAAGGCTCCCACACTGAGCACACAAGTTACGCAGCCGACCGCTTCAAACAAGTCGACGGGTTCTATGC
ACGCGACCTTACCACCAAAGCAAGGGCTACCGCCCCGACCACACGGAATCTCCTGACCACGCCCAAGTTT
ACCGTGGCGTGGGATTGGGTGCCAAAGCGCCCCAGCGTGTGTACTATGACCAAGTGGCAGGAAGTGGATG
AGATGCTCCGATCCGAATACGGAGGTTCCTTCCGCTTTAGCAGTGATGCCATATCAACCACCTTTACTAC
CAACCTTACAGAGTATCCGCTGAGTAGAGTTGATCTGGGAGATTGTATTGGGAAGGACGCTAGAGATGCA
ATGGACCGCATTTTCGCAAGGAGGTACAATGCAACTCACATTAAGGTGGGTCAACCCCAATACTATCTGG
CCAATGGGGGATTCCTGATAGCCTACCAGCCCCTGCTGTCTAACACGCTTGCCGAATTGTACGTCAGGGA
GCACCTGCGGGAGCAAAGCAGAAAACCCCCAAACCCCACCCCACCACCCCCGGGAGCTTCAGCCAACGCC
AGCGTCGAAAGAATCAAGACTACTTCCTCCATCGAGTTTGCCAGACTGCAATTTACATACAACCATATAC
AGAGACACGTAAACGATATGCTGGGCCGGGTTGCAATCGCATGGTGTGAACTGCAGAATCATGAGCTCAC
ATTGTGGAACGAGGCAAGAAAGCTCAATCCTAACGCTATTGCCTCCGCCACAGTTGGTCGGAGGGTGAGT
GCCAGAATGCTTGGCGATGTGATGGCAGTGTCAACCTGTGTTCCAGTTGCCGCCGACAACGTGATTGTGC
AAAATAGCATGCGGATCTCCAGCCGGCCCGGAGCATGTTATTCACGGCCACTCGTTAGCTTTAGGTACGA
GGATCAGGGGCCGCTGGTAGAGGGCCAACTGGGCGAGAATAACGAGCTGAGACTCACTCGCGACGCAATA
GAACCCTGCACTGTGGGCCATAGAAGGTACTTCACCTTTGGCGGAGGGTATGTATATTTTGAGGAGTACG
CTTATTCTCACCAACTGTCCCGCGCCGATATTACCACCGTGTCTACTTTTATTGACCTGAATATCACCAT
GTTGGAAGACCACGAATTCGTACCTTTGGAGGTGTACACTAGACACGAAATAAAAGATAGCGGTTTGCTG
GACTATACGGAAGTCCAGCGAAGAAACCAACTCCATGACCTGAGATTCGCGGACATTGATACGGTAATTC
ATGCTGACGCTAATGCCGCGATGTTTGCAGGACTTGGTGCCTTTTTCGAAGGAATGGGTGATCTCGGTAG
GGCTGTGGGGAAGGTGGTCATGGGCATTGTGGGAGGCGTAGTATCCGCTGTCAGTGGGGTTTCTTCATTT
ATGTCTAATCCATTTGGGGCCTTGGCCGTGGGGCTGCTTGTGTTGGCCGGATTGGCGGCTGCGTTCTTTG
CGTTTCGCTACGTCATGAGGCTCCAGTCTAACCCGATGAAAGCACTGTATCCCCTCACTACTAAAGAGCT
GAAGAACCCAACCAACCCAGACGCCTCAGGCGAGGGGGAAGAAGGTGGGGACTTCGACGAAGCCAAGCTT
GCTGAAGCCAGAGAGATGATTAGATACATGGCCTTGGTCTCTGCAATGGAGCGAACCGAGCATAAAGCCA
AAAAAAAGGGAACCTCAGCCCTGCTGAGTGCCAAAGTTACAGATATGGTCATGAGGAAACGCCGCAACAC
GAACTACACACAAGTACCTAACAAAGATGGGGACGCAGATGAGGACGACCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100815 representing NP_044629
Red=Cloning sites Green=Tags

MRQGAPARGRRWFVVWALLGLTLGVLVASAAPSSPGTPGVAAATQAANGGPATPAPPAPGAPPTGDPKPK
KNRKPKPPKPPRPAGDNATVAAGHATLREHLRDIKAENTDANFYVCPPPTGATVVQFEQPRRCPTRPEGQ
NYTEGIAVVFKENIAPYKFKATMYYKDVTVSQVWFGHRYSQFMGIFEDRAPVPFEEVIDKINAKGVCRST
AKYVRNNLETTAFHRDDHETDMELKPANAATRTSRGWHTTDLKYNPSRVEAFHRYGTTVNCIVEEVDARS
VYPYDEFVLATGDFVYMSPFYGYREGSHTEHTSYAADRFKQVDGFYARDLTTKARATAPTTRNLLTTPKF
TVAWDWVPKRPSVCTMTKWQEVDEMLRSEYGGSFRFSSDAISTTFTTNLTEYPLSRVDLGDCIGKDARDA
MDRIFARRYNATHIKVGQPQYYLANGGFLIAYQPLLSNTLAELYVREHLREQSRKPPNPTPPPPGASANA
SVERIKTTSSIEFARLQFTYNHIQRHVNDMLGRVAIAWCELQNHELTLWNEARKLNPNAIASATVGRRVS
ARMLGDVMAVSTCVPVAADNVIVQNSMRISSRPGACYSRPLVSFRYEDQGPLVEGQLGENNELRLTRDAI
EPCTVGHRRYFTFGGGYVYFEEYAYSHQLSRADITTVSTFIDLNITMLEDHEFVPLEVYTRHEIKDSGLL
DYTEVQRRNQLHDLRFADIDTVIHADANAAMFAGLGAFFEGMGDLGRAVGKVVMGIVGGVVSAVSGVSSF
MSNPFGALAVGLLVLAGLAAAFFAFRYVMRLQSNPMKALYPLTTKELKNPTNPDASGEGEEGGDFDEAKL
AEAREMIRYMALVSAMERTEHKAKKKGTSALLSAKVTDMVMRKRRNTNYTQVPNKDGDADEDDL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001806
ORF Size 2712 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001806.1, NP_044629
RefSeq ORF 2712 bp
Locus ID 2703455
UniProt ID P04413
MW 100.3 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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