UL19 (NC_001806) Virus Tagged ORF Clone

CAT#: VC100806

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for major capsid protein [Human herpesvirus 1], codon optimized for human cell expression, NP_044620



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CNY 20,710.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100806 represents NCBI reference of NP_044620 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTCCAAACAGAGATCCGCCTGGTTACCGGTACGCAGCCGCTATGGTGCCAACCGGCTCCCTCC
TGTCTACCATTGAAGTGGCAAGCCATAGAAGACTTTTTGACTTCTTCTCACGGGTGAGGAGCGACGCTAA
TTCTCTGTACGATGTCGAGTTCGACGCCCTCCTGGGCTCATATTGTAACACCCTGTCACTGGTGAGATTC
CTCGAGCTGGGCTTGAGCGTTGCGTGTGTCTGTACAAAGTTCCCAGAGCTTGCCTATATGAACGAGGGGA
GAGTTCAGTTCGAAGTGCACCAGCCGCTTATCGCCCGGGATGGCCCCCACCCCATCGAGCAGCCCACGCA
TAACTACATGACGAAAATTATCGACAGGAGAGCCTTGAATGCTGCTTTTTCTCTGGCCACCGAGGCCATC
GCCCTGCTGACAGGCGAAGCGCTGGATGGAACTGGAATCGGAGCACATCGGCAACTCCGAGCAATTCAGC
AACTTGCCCGGAATGTACAGGCAGTGCTGGGGGCCTTCGAGAGAGGGACGGCTGACCAGATGCTGCATGT
ACTGCTCGAAAAAGCTCCTCCACTCGCCCTGCTGCTCCCCATGCAACGGTACTTGGATAACGGAAGGCTT
GCTACACGAGTAGCCAGAGCAACGCTGGTGGCAGAGCTGAAACGCAGCTTTTGTGAAACCTCCTTCTTCC
TGGGAAAAGCCGGACATCGGAGGGAGGCAGTGGAAGCCTGGCTGGTGGACCTTACTACCGCTACTCAGCC
CAGCGTCGCGGTCCCCAGACTGACTCACGCCGACACACGCGGGCGGCCAGTCGACGGCGTGCTTGTGACT
ACGGCCCCTATCAAGCAGCGCCTGCTGCAATCCTTTCTGAAAGTGGAGGATACCGAAGCCGATGTTCCCG
TTACCTACGGTGAGATGGTACTCAACGGAGCCAACCTCGTGACCGCCCTGGTTATGGGAAAAGCGGTTAG
GAGCCTCGATGATGTCGGGCGCCATCTCCTCGAAATGCAGGAGGAACAGTTGGACTTGAACAGACAAACG
CTGGATGAGCTCGAATCAGCCCCGCAGACTACCAGGGTTAGGGCAGATCTCGTGTCCATAGGAGAAAAGC
TGGTATTTCTGGAGGCGCTGGAAAAGAGAATTTACGCCGCCACTAACGTTCCATATCCTCTGGTGGGCGC
CATGGACCTCACGTTTGTGCTGCCCCTGGGCCTCTTCAATCCTGTGATGGAGAGATTTGCCGCTCATGCG
GGGGACCTGGTGCCTGCCCCCGGCCACCCTGATCCTCGCGCTTTCCCTCCTCGCCAGCTCTTCTTTTGGG
GCAAAGACAGGCAGGTATTGCGACTGTCCTTGGAACACGCTATCGGTACTGTGTGCCATCCTTCCCTGAT
GAACGTCGACGCAGCGGTGGGCGGCCTGAACCGCGATCCAGTTGAGGCTGCCAACCCATATGGTGCATAC
GTGGCTGCTCCCGCTGGCCCAGCGGCAGACATGCAGCAGCTTTTTCTCAACGCCTGGGGGCAACGGCTCG
CCCACGGACGGGTACGGTGGGTCGCCGAAGGCCAGATGACCCCTGAACAGTTTATGCAGCCAGATAATGC
CAATCTTGCGCTGGAACTGCATCCCGCCTTCGATTTCTTCGTGGGGGTGGCCGACGTCGAGCTGCCGGGT
GGAGATGTACCTCCAGCAGGGCCTGGAGAGATCCAGGCTACTTGGCGGGTCGTGAACGGTAACCTGCCCC
TTGCACTCTGCCCTGCAGCATTCCGCGACGCCAGGGGGCTGGAACTGGGAGTGGGTCGACACGCCATGGC
GCCCGCTACCATCGCTGCCGTGAGGGGTGCCTTCGATGACAGGAATTACCCGGCCGTGTTCTATCTCTTG
CAGGCCGCGATTCACGGCTCTGAGCATGTGTTTTGTGCATTGGCCAGGCTGGTGGTGCAGTGTATTACCA
GCTATTGGAATAATACCCGCTGTGCCGCTTTTGTGAATGACTACAGCTTGGTTAGCTATGTCGTTACTTA
TCTGGGGGGCGATCTCCCTGAAGAGTGCATGGCAGTGTATCGCGACTTGGTGGCCCACGTAGAGGCTCTT
GCACAACTGGTGGACGATTTTACACTGACTGGACCAGAGCTGGGCGGGCAGGCACAGGCAGAACTCAACC
ATCTGATGAGGGACCCTGCACTCTTGCCTCCTCTGGTGTGGGATTGCGACGCACTTATGAGGAGGGCAGC
CCTCGACCGCCACCGCGATTGTCGAGTAAGCGCTGGAGGACATGACCCAGTGTATGCAGCGGCTTGCAAC
GTTGCAACAGCCGATTTCAACAGAAATGACGGTCAACTGCTTCATAATACCCAAGCTAGAGCCGCCGACG
CTGCTGACGATAGGCCCCATAGGGGTGCCGACTGGACAGTCCACCACAAAATTTATTACTACGTGATGGT
CCCAGCATTTTCCCGCGGCCGGTGCTGTACCGCCGGCGTGCGCTTTGATCGGGTGTACGCCACTTTGCAG
AACATGGTGGTGCCTGAAATTGCTCCAGGGGAAGAGTGCCCCTCCGACCCGGTGACTGACCCCGCCCACC
CCCTTCACCCTGCTAACCTCGTGGCAAACACCGTAAACGCCATGTTTCACAATGGTAGAGTGGTTGTCGA
CGGGCCTGCGATGCTGACACTTCAGGTTCTGGCTCACAACATGGCAGAACGCACCACCGCACTCCTGTGT
AGCGCGGCGCCGGACGCTGGAGCAAATACTGCCTCCACCACCAATATGCGGATATTTGACGGGGCACTGC
ACGCCGGCATACTGTTGATGGCCCCCCAGCACCTGGATCACACAATCCAGAATGGCGACTATTTTTATCC
CCTGCCAGTGCATGCTTTGTTTGCGGGCGCGGACCATGTGGCTAACGCTCCTAATTTCCCCCCAGCACTG
CGAGACCTTAGCCGCCAGGTCCCACTTGTTCCACCCGCCCTGGGGGCAAATTACTTTTCATCAATTCGCC
AGCCCGTGGTCCAACACGTCAGGGAATCCGCCGCCGGGGAGAACGCGCTGACATATGCACTGATGGCAGG
GTATTTTAAGATTTCCCCTGTCGCTCTGCATCACCAGCTGAAGACAGGCCTGCATCCAGGGTTCGGGTTC
ACTGTGGTTAGACAGGATCGCTTTGTTACAGAAAACGTGCTGTTTAGCGAAAGGGCCTCCGAAGCATACT
TTCTGGGGCAGCTGCAGGTAGCAAGACACGAAACGGGTGGAGGTGTCAATTTCACCCTGACTCAACCCAG
AGCCAACGTGGATCTGGGCGTGGGGTATACCGCTGTGGTGGCCACCGCTACTGTTCGGAATCCCGTAACT
GATATGGGGAACCTGCCCCAGAACTTCTATCTCGGCCGCGGTGCCCCGCCGCTGCTGGATAATGCAGCTG
CCGTATATCTGCGCAATGCCGTCGTGGCTGGGAACAGACTGGGACCAGCCCAACCGGTTCCTGTTTTTGG
ATGTGCTCAAGTGCCAAGACGGGCCGGCATGGACCATGGCCAGGATGCTGTTTGCGAATTCATCGCTACA
CCCGTGTCAACCGACGTGAACTACTTCAGAAGGCCCTGCAATCCCCGCGGCCGCGCAGCCGGAGGGGTCT
ACGCCGGAGATAAAGAGGGCGATGTCACAGCATTGATGTACGATCATGGGCAGTCTGATCCCAGCCGAGC
TTTTGCCGCCACAGCGAACCCCTGGGCATCTCAACGGTTCTCATACGGGGATCTCCTGTACAACGGGGCC
TATCACCTGAACGGAGCATCTCCTGTGCTCTCACCATGTTTTAAGTTCTTCACGTCCGCTGACATTGCCG
CTAAACATCGATGTCTGGAGAGACTGATCGTGGAGACAGGCTCAGCTGTGAGCACGGCCACAGCCGCTAG
TGACGTACAATTTAAAAGGCCACCTGGATGTCGGGAGTTGGTCGAAGACCCCTGTGGCCTTTTCCAGGAA
GCCTATCCACTCACTTGCGCGTCAGATCCTGCTTTGCTGCGCTCTGCTCGAAATGGCGAGGCCCATGCAC
GGGAGACACACTTTGCACAGTATCTGGTGTATGATGCCAGTCCTCTGAAAGGACTGGCCCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100806 representing NP_044620
Red=Cloning sites Green=Tags

MAAPNRDPPGYRYAAAMVPTGSLLSTIEVASHRRLFDFFSRVRSDANSLYDVEFDALLGSYCNTLSLVRF
LELGLSVACVCTKFPELAYMNEGRVQFEVHQPLIARDGPHPIEQPTHNYMTKIIDRRALNAAFSLATEAI
ALLTGEALDGTGIGAHRQLRAIQQLARNVQAVLGAFERGTADQMLHVLLEKAPPLALLLPMQRYLDNGRL
ATRVARATLVAELKRSFCETSFFLGKAGHRREAVEAWLVDLTTATQPSVAVPRLTHADTRGRPVDGVLVT
TAPIKQRLLQSFLKVEDTEADVPVTYGEMVLNGANLVTALVMGKAVRSLDDVGRHLLEMQEEQLDLNRQT
LDELESAPQTTRVRADLVSIGEKLVFLEALEKRIYAATNVPYPLVGAMDLTFVLPLGLFNPVMERFAAHA
GDLVPAPGHPDPRAFPPRQLFFWGKDRQVLRLSLEHAIGTVCHPSLMNVDAAVGGLNRDPVEAANPYGAY
VAAPAGPAADMQQLFLNAWGQRLAHGRVRWVAEGQMTPEQFMQPDNANLALELHPAFDFFVGVADVELPG
GDVPPAGPGEIQATWRVVNGNLPLALCPAAFRDARGLELGVGRHAMAPATIAAVRGAFDDRNYPAVFYLL
QAAIHGSEHVFCALARLVVQCITSYWNNTRCAAFVNDYSLVSYVVTYLGGDLPEECMAVYRDLVAHVEAL
AQLVDDFTLTGPELGGQAQAELNHLMRDPALLPPLVWDCDALMRRAALDRHRDCRVSAGGHDPVYAAACN
VATADFNRNDGQLLHNTQARAADAADDRPHRGADWTVHHKIYYYVMVPAFSRGRCCTAGVRFDRVYATLQ
NMVVPEIAPGEECPSDPVTDPAHPLHPANLVANTVNAMFHNGRVVVDGPAMLTLQVLAHNMAERTTALLC
SAAPDAGANTASTTNMRIFDGALHAGILLMAPQHLDHTIQNGDYFYPLPVHALFAGADHVANAPNFPPAL
RDLSRQVPLVPPALGANYFSSIRQPVVQHVRESAAGENALTYALMAGYFKISPVALHHQLKTGLHPGFGF
TVVRQDRFVTENVLFSERASEAYFLGQLQVARHETGGGVNFTLTQPRANVDLGVGYTAVVATATVRNPVT
DMGNLPQNFYLGRGAPPLLDNAAAVYLRNAVVAGNRLGPAQPVPVFGCAQVPRRAGMDHGQDAVCEFIAT
PVSTDVNYFRRPCNPRGRAAGGVYAGDKEGDVTALMYDHGQSDPSRAFAATANPWASQRFSYGDLLYNGA
YHLNGASPVLSPCFKFFTSADIAAKHRCLERLIVETGSAVSTATAASDVQFKRPPGCRELVEDPCGLFQE
AYPLTCASDPALLRSARNGEAHARETHFAQYLVYDASPLKGLAL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001806
ORF Size 4122 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001806.1, NP_044620
RefSeq ORF 4122 bp
Locus ID 2703368
UniProt ID P04413
MW 149.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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