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E2B (NC_011202) Virus Tagged ORF Clone
CAT＃: VC100294
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase [Human adenovirus B], codon optimized for human cell expression, YP_002213842
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CNY 18,050.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC100294 represents NCBI reference of YP_002213842 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATTGGTGCAGGCTCACGGCGCGGGGTCCTTTCACCCAGAGACCTCCGACACAAGGAGGCAGTCCA CTCGGGGTGGCGCTAGGAAGAGCTCACAGAGCGCTGTGCCCGAACCAGCCCGCGCACGGAGGAGAAGGGC TACCACTAGCCACGCATTGGGAAGCGGTACAACTGCCGCTGCCAGAAGGGCATCCCCTTCACCACTGCTT TCCATGCAGGAGGCGAGAGAACCCCCCCCAATTAAAAGAAAGAACAAGGGAACTGTGGTCGCCCCCAAGG GTCACGGCACACTGCAGGCGATAGACGTGAGTACCAATGAGCCAGTCGAGATCAAATACCACCTGAACTT GCCCCACGCTCTGGAGAAGATCATGCAGGTCAACTTGTTGTCCCTGCCCACTAACCTTTCCCCTCAGCGC CTGAGGACCCTGGACTCCAGTAGCCTGAGAGCCTTGGTTCTGCAGCTTAGACCATCTCGAGCCGAGGTTT GGACCTGCCTGCCCAGAGGGCTCGTCTCTATGACGACTATCGAGACTGAAGACGAGCAAGCAGATACCGC CAATATCGAGGAACACGAAATTCAAAGCCCCGGTCTGGGTTTTCCTCTCAAATTTCTCGTCAAGGGTAGC CAAGTGCAGCTGATTCACGAAATTCAGCCGGTGCAGAGATGCGACTATTGCGGAAGACTGTATAAACACA AGCACGAATGCAGCGCCCGCCGGCGAAACTTCTATTTCCATCACATTAATAGCCAATCATCTAACTGGTG GCAGGAGATACAGTTTTTTCCCATCGGATCCCACCCAAGAACCGAACGCCTGTTCCTGACCTACGATGTG GAGACCTACACCTGGATGGGATCATTTGGGAAGCAGCTCGTGCCTTTTATGCTTGTCATGAAGTTGTCAG GGGAGGAGAGATTGGTGAATATGGCCCATGACCTGGCTATAAAACTGAAATGGGATCGATGGCAGCAGGA TCCCAGAACCTTTTACTGCGTTACACCCGAGAAAATGGCTATTGGGCGCCATTTTAGGCAGTACCGAGAC CAACTGCAGACGGCCCTTGCAGTGGATCTCTGGTCAAGTTTTCTGCACGCGAACCCCCATCTGCATGAAT GGGCACTGGAGCATTACGCACTGAGCGATCCTACCGATCTCACTTTTGACGAACTGAGCAAGCTTCCGCA TGTGCGAGGCACCCCCCGATTCATTGAACTGTACATCGTTGGACATAACATTAACGGATTTGACGAGATC GTTTTGGCCGCCCAGGTGATTAACAACCGGGCCGAAGTGCCACAGCCTTTCAGGATCACCAGGAATTTCA TGCCTAGAGCCGGGAAGATCCTTTTTAATGACGTAACATTTGCTCTCCCAAACCCTGCCTACAAGAAGAG AGTTGATTTCCAGCTGTGGGAGCAAGGAGCTTGTGATGATATCGATTTCAAATATCAATTTCTGAAAGTG ATGGTTAGAGACACTTTCGCCCTCACACATGCTTCCCTTCGGAAAGCCGCCCAAGCCTACTCCCTGCCCG TGGAGAAAGGTTGCTGTCCTTATAAAGCAGTGAATCAGTTCTACATGCTGGGGAGCTACCGCGCTGACAA AGACGGTTTTCCCCTCGAGGAATATTGGAAGGACCGGGAGGAGTACCTGCTGAACCGGGAGTTGTGGGAG AAAAAAAATCAACCTGGCTATGATATTATACAGGAGACCTTGGACTACTGCGCCCTGGACGTCCTGGTGA CGGCGGAACTCGTTACGAAGCTGCAAGAGTCATACGCCCATTTTGTCCGGGACTCCGTCGGCTTGCCTCA CGCTCATTTTAACATATTTCAGCGCCCCACTATCAGCTCAAACAGTCATGCAATATTTCGACAAATAGTG TATCGAACTGAGAAGCCAAACCGCACCAACCTCGGCCCGGGACTGCTCGCTCCCTCTCATGAGTTGTACG ATTACGTCCGGGCCTCAATACGCGGCGGGAGATGTTATCCCACCTACATTGGCATCTTTGAGGATCCGAT ATACGTGTATGATATCTGTGGTATGTATGCCTCCGCACTCACGCACCCAATGCCTTGGGGCAGCCCGCTG AATCCTTATGAGAGAGCCCTGGCCGCAAGAGAGTGGCAGATGGCTCTTGATGACCCGACACCAATCTCAT ATTTCGACAAAGACCTTCTCCCTGGCATCTTCACAATGGACGCTGATCCCCCAGATGAGTTGATGCTTGA CCCCTTGCCCCCATTCTGTTCCCGCAAGGGAGGACGGCTGTGCTGGACTAATGAGCCACTGCGGGGCGAA GTAGCCACCTCAGTCGACCTGATCACACTGCACAACAGGGGTTGGCAAGTGAGGATCGTGCCTGACGAAC TCACGACCATATTCCCAGAGTGGAAATGCCTGGCCAGAGAGTACGTGCAGTTGAATATCGCAGCTAAAGA ACGCGCTGACAAAGAGAAAAACCAGACAATGCGCTCAATCGCGAAACTGCTGAGTAATGCACTGTATGGG AGCTTTGCTACTAAACTGGATAACAAAAAGATTGTGTTTTCAGACCAGATGGATGAAAACCTCATGAAGG GCATTTCAACCGGCACCGTAAACATTAAGTCAAGTTCTTTTTTGGAAACCGATAATTTGAGTGCCGAAGT TATGCCAGCATTCGAGCGGGAGTACCTCCCCCAACAGCTGACGTTTCTTGACTCCGACCCTGAAGACAGC GAGGAGGAGCAACGAACCGCCCCATTTTATACGCCCCCCGCCGGGACCCCTGGCCACGTGAAGTACACTT ATAAGCCTATTACATTCCTCGACGTGGAGGAAGGTGACATGTGTCTGCATACTTTGGAGAAGGTCGACCC GCTTGTGGATAATGACAGGTACCCTTCTCATATTGCGAGCTTTGTGCTCGCGTGGACTAGGGCTTTCGTG AGTGAGTGGAGTGAGTTTTTGTATGAAGAAGACCGAGGGACACCCCTCGAAAAGAGACCTGTGAAGAGCG TGTACGGGGATACTGACTCACTCTTCGTTACCCAGCGCGGACACGAACTTATGGAAACTAAAGGCAAGAA GAGAATCAAGAAGTACGGCGGTAAGCTGGTCTTCGACCCCAACGAACCCGATCTGACCTGGCTGGTGGAG TGCGAAACGGTGTGCGCGAGCTGCGGTGCCGACGCATACTCTCCCGAATCCGTCTTCCTCGCTCCTAAAC TGTATGCTCTCAAGTGCCTGTATTGTCCTGTTTGTAAGAAGACTTCTAAGGGTAAACTGAGGGCCAAAGG CCATGCTGCCGAGGCTTTGAATTACGAACTGATGGTTAACTGTTACCTGGCCGACATCCAGGGGGCCAAG CGACAAAAGTTTAGTACATCTAGGATGAGCCTGAAGCGAACCCTGGCATCCGCTCAAGCCGGCGCTCACC CCTTTACGGTGACCGAAACGACACTGACCAGAACACTGAGACCATGGAAGGATCGGACTCTGGCTGCACT CGACGCACACCGACTTATCCCTTACTCCAGGAGTAGACCAAACCCGCGCAATGAGGAGGTCTGCTGGATC GAAATGCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100294 representing YP_002213842
Red=Cloning sites Green=Tags MALVQAHGAGSFHPETSDTRRQSTRGGARKSSQSAVPEPARARRRRATTSHALGSGTTAAARRASPSPLL SMQEAREPPPIKRKNKGTVVAPKGHGTLQAIDVSTNEPVEIKYHLNLPHALEKIMQVNLLSLPTNLSPQR LRTLDSSSLRALVLQLRPSRAEVWTCLPRGLVSMTTIETEDEQADTANIEEHEIQSPGLGFPLKFLVKGS QVQLIHEIQPVQRCDYCGRLYKHKHECSARRRNFYFHHINSQSSNWWQEIQFFPIGSHPRTERLFLTYDV ETYTWMGSFGKQLVPFMLVMKLSGEERLVNMAHDLAIKLKWDRWQQDPRTFYCVTPEKMAIGRHFRQYRD QLQTALAVDLWSSFLHANPHLHEWALEHYALSDPTDLTFDELSKLPHVRGTPRFIELYIVGHNINGFDEI VLAAQVINNRAEVPQPFRITRNFMPRAGKILFNDVTFALPNPAYKKRVDFQLWEQGACDDIDFKYQFLKV MVRDTFALTHASLRKAAQAYSLPVEKGCCPYKAVNQFYMLGSYRADKDGFPLEEYWKDREEYLLNRELWE KKNQPGYDIIQETLDYCALDVLVTAELVTKLQESYAHFVRDSVGLPHAHFNIFQRPTISSNSHAIFRQIV YRTEKPNRTNLGPGLLAPSHELYDYVRASIRGGRCYPTYIGIFEDPIYVYDICGMYASALTHPMPWGSPL NPYERALAAREWQMALDDPTPISYFDKDLLPGIFTMDADPPDELMLDPLPPFCSRKGGRLCWTNEPLRGE VATSVDLITLHNRGWQVRIVPDELTTIFPEWKCLAREYVQLNIAAKERADKEKNQTMRSIAKLLSNALYG SFATKLDNKKIVFSDQMDENLMKGISTGTVNIKSSSFLETDNLSAEVMPAFEREYLPQQLTFLDSDPEDS EEEQRTAPFYTPPAGTPGHVKYTYKPITFLDVEEGDMCLHTLEKVDPLVDNDRYPSHIASFVLAWTRAFV SEWSEFLYEEDRGTPLEKRPVKSVYGDTDSLFVTQRGHELMETKGKKRIKKYGGKLVFDPNEPDLTWLVE CETVCASCGADAYSPESVFLAPKLYALKCLYCPVCKKTSKGKLRAKGHAAEALNYELMVNCYLADIQGAK RQKFSTSRMSLKRTLASAQAGAHPFTVTETTLTRTLRPWKDRTLAALDAHRLIPYSRSRPNPRNEEVCWI EMP TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_011202 |
| ORF Size | 3579 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_011202.1, YP_002213842 |
| RefSeq ORF | 3579 bp |
| Locus ID | 6870501 |
| MW | 136.2 kDa |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC100290 | Myc-DDK-tagged ORF clone of viral ORF for control protein E1A [Human adenovirus B], codon optimized for human cell expression, YP_002213798 |
CNY 3,800.00 |
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| VC100291 | Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 19K [Human adenovirus B], codon optimized for human cell expression, YP_002213799 |
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| VC100292 | Myc-DDK-tagged ORF clone of viral ORF for control protein E1B 55K [Human adenovirus B], codon optimized for human cell expression, YP_002213800 |
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| VC100293 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein IX [Human adenovirus B], codon optimized for human cell expression, YP_002213801 |
CNY 3,800.00 |
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| VC100295 | Myc-DDK-tagged ORF clone of viral ORF for terminal protein precursor pTP [Human adenovirus B], codon optimized for human cell expression, YP_002213843 |
CNY 7,890.00 |
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| VC100296 | Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein IVa2 [Human adenovirus B], codon optimized for human cell expression, YP_002213841 |
CNY 5,510.00 |
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| VC100297 | Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus B], codon optimized for human cell expression, YP_002213828 |
CNY 3,800.00 |
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| VC100298 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVIII [Human adenovirus B], codon optimized for human cell expression, YP_002213818 |
CNY 3,800.00 |
|
| VC100299 | Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 22K [Human adenovirus B], codon optimized for human cell expression, YP_002213817 |
CNY 3,800.00 |
|
| VC100300 | Myc-DDK-tagged ORF clone of viral ORF for protein 33K [Human adenovirus B], codon optimized for human cell expression, YP_002213816 |
CNY 3,800.00 |
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| VC100301 | Myc-DDK-tagged ORF clone of viral ORF for hexon assembly protein 100K [Human adenovirus B], codon optimized for human cell expression, YP_002213815 |
CNY 12,350.00 |
|
| VC100302 | Myc-DDK-tagged ORF clone of viral ORF for protease [Human adenovirus B], codon optimized for human cell expression, YP_002213813 |
CNY 3,800.00 |
|
| VC100303 | Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus B], codon optimized for human cell expression, YP_002213812 |
CNY 14,250.00 |
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| VC100304 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pVI [Human adenovirus B], codon optimized for human cell expression, YP_002213811 |
CNY 3,800.00 |
|
| VC100305 | Myc-DDK-tagged ORF clone of viral ORF for core protein precursor pX [Human adenovirus B], codon optimized for human cell expression, YP_002213810 |
CNY 3,800.00 |
|
| VC100306 | Myc-DDK-tagged ORF clone of viral ORF for core protein V [Human adenovirus B], codon optimized for human cell expression, YP_002213809 |
CNY 4,180.00 |
|
| VC100307 | Myc-DDK-tagged ORF clone of viral ORF for core protein precursor pVII [Human adenovirus B], codon optimized for human cell expression, YP_002213808 |
CNY 3,800.00 |
|
| VC100308 | Myc-DDK-tagged ORF clone of viral ORF for penton base [Human adenovirus B], codon optimized for human cell expression, YP_002213807 |
CNY 6,750.00 |
|
| VC100309 | Myc-DDK-tagged ORF clone of viral ORF for protein 136K [Human adenovirus B], codon optimized for human cell expression, YP_002213804 |
CNY 3,800.00 |
|
| VC100310 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein precursor pIIIa [Human adenovirus B], codon optimized for human cell expression, YP_002213806 |
CNY 7,030.00 |
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| VC100311 | Myc-DDK-tagged ORF clone of viral ORF for encapsidation protein 52K [Human adenovirus B], codon optimized for human cell expression, YP_002213805 |
CNY 4,660.00 |
|
| VC100312 | Myc-DDK-tagged ORF clone of viral ORF for single-stranded DNA-binding protein [Human adenovirus B], codon optimized for human cell expression, YP_002213852 |
CNY 6,270.00 |
|
| VC100313 | Myc-DDK-tagged ORF clone of viral ORF for control protein E3 125K [Human adenovirus B], codon optimized for human cell expression, YP_002213819 |
CNY 3,800.00 |
|
| VC100314 | Myc-DDK-tagged ORF clone of viral ORF for control protein E3 147K [Human adenovirus B], codon optimized for human cell expression, YP_002213826 |
CNY 3,800.00 |
|
| VC100315 | Myc-DDK-tagged ORF clone of viral ORF for membrane protein E3 RID-beta [Human adenovirus B], codon optimized for human cell expression, YP_002213825 |
CNY 3,800.00 |
|
| VC100316 | Myc-DDK-tagged ORF clone of viral ORF for membrane protein E3 RID-alpha [Human adenovirus B], codon optimized for human cell expression, YP_002213824 |
CNY 3,800.00 |
|
| VC100317 | Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-gamma [Human adenovirus B], codon optimized for human cell expression, YP_002213823 |
CNY 3,800.00 |
|
| VC100318 | Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-beta [Human adenovirus B], codon optimized for human cell expression, YP_002213822 |
CNY 3,800.00 |
|
| VC100319 | Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 gp19K [Human adenovirus B], codon optimized for human cell expression, YP_002213821 |
CNY 3,800.00 |
|
| VC100320 | Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein E3 CR1-alpha [Human adenovirus B], codon optimized for human cell expression, YP_002213820 |
CNY 3,800.00 |
|
| VC100321 | Myc-DDK-tagged ORF clone of viral ORF for protein U, partial [Human adenovirus B], codon optimized for human cell expression, YP_002213845 |
CNY 3,800.00 |
|
| VC100322 | Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf6/7 [Human adenovirus B], codon optimized for human cell expression, YP_002213846 |
CNY 3,800.00 |
|
| VC100323 | Myc-DDK-tagged ORF clone of viral ORF for control protein E4 34K [Human adenovirus B], codon optimized for human cell expression, YP_002213847 |
CNY 3,800.00 |
|
| VC100324 | Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf4 [Human adenovirus B], codon optimized for human cell expression, YP_002213848 |
CNY 3,800.00 |
|
| VC100325 | Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf3 [Human adenovirus B], codon optimized for human cell expression, YP_002213849 |
CNY 3,800.00 |
|
| VC100326 | Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf2 [Human adenovirus B], codon optimized for human cell expression, YP_002213850 |
CNY 3,800.00 |
|
| VC100327 | Myc-DDK-tagged ORF clone of viral ORF for control protein E4orf1 [Human adenovirus B], codon optimized for human cell expression, YP_002213851 |
CNY 1,200.00 |
