pVIII (AC_000017) Virus Tagged ORF Clone

CAT#: VC100021

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for pVIII [Human adenovirus 1], codon optimized for human cell expression, AP_000518



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CNY 3,800.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100021 represents NCBI reference of AP_000518 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTAAAGAAATCCCCACACCTTATATGTGGTCATATCAGCCGCAGATGGGACTGGCTGCCGGGGCAG
CCCAGGATTATTCAACCCGCATCAACTACATGTCAGCTGGCCCTCACATGATTAGCCGCGTTAATGGTAT
CAGAGCCCACAGGAACAGAATACTGCTCGAACAGGCCGCCATAACCACTACTCCTCGCAATAATCTCAAC
CCCAGAAGCTGGCCCGCGGCCCTCGTCTACCAGGAGTCTCCAGCTCCCACCACCGTGGTGCTGCCTCGCG
ATGCTCAAGCGGAGGTTCAAATGACCAACAGTGGTGCGCAACTCGCGGGGGGTTTCCGCCACAGGGTGCG
CTCCCCCGGACAAGGGATTACTCACCTTAAAATAAGGGGAAGGGGCATCCAGCTCAACGACGAATCTGTG
TCTAGCAGTCTTGGCCTGCGGCCAGATGGAACATTCCAGATAGGAGGCGCTGGAAGATCTTCTTTCACCC
CAAGACAGGCCATCCTGACTCTGCAGACCAGCAGTAGTGAACCACGGTCTGGAGGCATCGGAACACTGCA
GTTCATCGAAGAATTCGTGCCCTCTGTGTACTTTAACCCATTCTCCGGGCCGCCTGGACATTATCCTGAT
CAGTTCATCCCTAACTTTGATGCCGTGAAAGATTCAGCGGATGGCTATGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100021 representing AP_000518
Red=Cloning sites Green=Tags

MSKEIPTPYMWSYQPQMGLAAGAAQDYSTRINYMSAGPHMISRVNGIRAHRNRILLEQAAITTTPRNNLN
PRSWPAALVYQESPAPTTVVLPRDAQAEVQMTNSGAQLAGGFRHRVRSPGQGITHLKIRGRGIQLNDESV
SSSLGLRPDGTFQIGGAGRSSFTPRQAILTLQTSSSEPRSGGIGTLQFIEEFVPSVYFNPFSGPPGHYPD
QFIPNFDAVKDSADGYD

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000017
ORF Size 681 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq AC_000017.1, AP_000518
RefSeq ORF 681 bp
MW 24.7 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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