AP3B2 (NM_001278511) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DEE48; EIEE48; NAPTB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278511, the custom clone sequence may differ by one or more nucleotides ATGTCGGCCGCCCCCGCCTACAGCGAAGACAAGGGCGGCTCCGCTGGCCCCGGGGAGCCCGAGTACGGCC ACGACCCCGCGAGCGGCGGCATCTTCTCCTCCGACTACAAGCGGCATGATGACCTGAAGGAGATGCTGGA CACCAACAAGGATTCTCTCAAGCTGGAGGCCATGAAGAGGATTGTGGCGATGATTGCCCGAGGAAAGAAT GCTTCAGACCTGTTTCCCGCGGTGGTGAAGAACGTGGCCTGTAAGAACATAGAGGATCCCAACCAGCTGA TTCGTGCCAGTGCCCTCCGTGTCCTCTCTAGCATCCGTGTGCCCATCATAGTGCCCATCATGATGCTAGC TATCAAGGAAGCCGCCTCGGACATGTCACCCTATGTGCGGAAAACAGCTGCCCACGCCATCCCTAAACTC TACAGTTTGGACTCTGACCAGAAGGATCAGCTGATAGAAGTCATTGAGAAGCTTCTGGCTGACAAGACCA CGCTGGTGGCGGGCAGTGTGGTGATGGCCTTTGAGGAGGTCTGCCCGGAGCGCATCGACCTGATTCACAA AAACTACCGGAAACTCTGTAACCTGCTGATCGACGTGGAGGAGTGGGGCCAGGTGGTCATCATCAGCATG CTCACCCGCTACGCCCGCACGCAGTTCCTGAGCCCCACCCAGAACGAATCCCTACTAGAGGAGAACGCGG AAAAAGCCTTCTACGGCTCAGAGGAGGACGAGGCCAAGGGCGCGGGGTCTGAGGAGACGGCCGCCGCGGC CGCCCCCTCCCGAAAGCCCTATGTCATGGACCCCGACCACCGGCTGCTGCTGCGCAACACCAAACCCCTG CTGCAGAGCCGCAGCGCCGCGGTGGTGATGGCGGTGGCGCAGCTCTACTTCCACCTGGCGCCCAAGGCGG AAGTGGGCGTCATCGCCAAGGCGCTGGTGCGCCTGCTGCGCAGCCACAGTGAGGTGCAGTACGTTGTGCT CCAGAACGTGGCCACCATGTCCATCAAGCGCCGGGGTATGTTTGAGCCCTACCTGAAGAGCTTCTACATC AGGTCCACCGACCCCACCCAGATTAAGATCCTGAAGCTGGAAGTGCTGACCAACCTGGCCAATGAGACCA ACATTCCTACTGTCCTACGGGAATTCCAGACCTATATTCGCAGCATGGACAAGGACTTTGTGGCAGCCAC AATCCAGGCCATTGGACGCTGTGCAACTAACATCGGCCGAGTCCGTGACACCTGCCTCAATGGCCTGGTG CAGCTGCTGTCCAACCGTGATGAGCTTGTGGTTGCAGAGTCAGTGGTCGTCATTAAGAAATTGCTACAGA TGCAGCCAGCACAACATGGAGAGATCATCAAACACTTGGCAAAGCTTACAGACAACATCCAGGTGCCCAT GGCCCGAGCCAGCATCCTGTGGCTCATCGGAGAGTACTGTGAGCATGTCCCCAGGATTGCACCTGATGTC TTAAGAAAAATGGCCAAGTCATTCACAGCAGAGGAGGATATTGTCAAGCTGCAGGTCATCAACCTGGCAG CCAAGCTCTACCTGACCAACTCTAAACAGACCAAGCTGCTGACCCAGTATGTGCTGAGTCTGGCCAAATA TGACCAGAACTATGATATTCGCGACCGGGCGCGCTTCACCCGGCAGCTCATCGTCCCTTCCGAGCAGGGT GGGGCCCTCAGCCGCCATGCCAAGAAGCTCTTCCTGGCACCCAAACCAGCTCCAGTCTTGGAGTCATCCT TCAAAGACCGGGACCACTTCCAGCTGGGCTCACTGTCCCACCTGCTTAATGCCAAGGCCACAGGCTACCA GGAGCTCCCAGACTGGCCGGAGGAAGCCCCAGACCCATCTGTGCGCAACGTGGAGGTACCTGAATGGACC AAGTGCTCAAATCGGGAGAAGAGAAAGGAGAAGGAAAAACCCTTCTACTCGGACTCTGAGGGGGAGTCAG GCCCCACGGAGTCCGCAGACAGTGACCCTGAGTCTGAGAGTGAATCGGACAGTAAGAGCAGCAGTGAGAG CGGCTCTGGGGAGTCCAGCAGTGAGTCCGACAATGAAGACCAGGATGAGGATGAGGAGAAAGGGAGAGGC AGTGAGAGTGAACAGAGTGAGGAGGATGGTAAGAGGAAGACAAAGAAGAAGGTGCCAGAGAGAAAAGGAG AAGCGTCATCCTCTGATGAGGGCAGCGATTCCAGCAGTAGCTCATCAGAGTCCGAGATGACATCGGAGTC CGAGGAGGAGCAGTTAGAACCTGCCTCCTGGAGCAGGAAAACACCTCCCAGCAGCAAAAGTGCTCCTGCA ACCAAGGAGATCTCCCTGCTTGATCTAGAGGATTTCACCCCTCCCAGTGTCCAGCCTGTGTCTCCCCCAG CAATTGTGTCTACCAGTCTGGCTGCTGACCTGGAGGGCCTGACACTCACAGACTCCACCCTGGTACCGTC GCTTCTGAGTCCAGTATCGGGTGTTGGGCGGCAGGAGCTGCTGCACCGGGTAGCTGGCGAGGGGCTGGCT GTGGACTACACCTTCAGCCGCCAACCTTTCTCCGGGGATCCCCACATGGTGTCCGTGCACATCCACTTCT CCAACAGCTCTGATACCCCCATCAAGGGCCTGCATGTGGGCACTCCCAAACTGCCTGCTGGCATCAGCAT CCAAGAATTTCCCGAAATTGAGTCCCTGGCACCTGGAGAATCTGCCACTGCTGTAATGGGCATTAATTTC TGTGACTCAACCCAGGCAGCCAACTTCCAGCTGTGCACCCAGACCCGACAGTTCTACGTCTCCATTCAGC CACCTGTTGGGGAGCTGATGGCCCCTGTGTTCATGAGTGAAAATGAGTTTAAGAAGGAACAGGGAAAGCT GATGGGCATGAATGAGATCACAGAGAAACTCATGCTGCCAGACACCTGTCGGAGTGACCACATTGTGGTG CAGAAAGTGACTGCCACTGCCAACCTGGGTCGTGTTCCTTGTGGGACATCTGATGAGTACAGGTTTGCAG GGAGGACACTGACTGGTGGAAGCCTCGTTCTGCTGACCCTGGATGCCCGGCCAGCTGGAGCTGCCCAGCT GACTGTCAACAGCGAGAAAATGGTGATTGGCACCATGCTGGTAAAGGATGTGATACAGGCTCTGACCCAG TGA
Restriction Sites	SgfI-MluI
ACCN	NM_001278511
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278511.1, NP_001265440.1
RefSeq Size	3675 bp
RefSeq ORF	3153 bp
Locus ID	8120
UniProt ID	Q13367
Protein Pathways	Lysosome
Gene Summary	Adaptor protein complex 3 (AP-3 complex) is a heterotrimeric protein complex involved in the formation of clathrin-coated synaptic vesicles. The protein encoded by this gene represents the beta subunit of the neuron-specific AP-3 complex and was first identified as the target antigen in human paraneoplastic neurologic disorders. The encoded subunit binds clathrin and is phosphorylated by a casein kinase-like protein, which mediates synaptic vesicle coat assembly. Defects in this gene are a cause of early-onset epileptic encephalopathy. [provided by RefSeq, Feb 2017] Transcript Variant: This variant (3) lacks two alternate in-frame exons compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1.

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