AASDH (NM_001286669) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACSF4; LYS2; NRPS998; NRPS1098
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286669, the custom clone sequence may differ by one or more nucleotides ATGCTAAATGATGGAAAAGAGAAATATGAAAAAGAAAAAATAAAAAGCATAAGTTCTGAGCATGTCAATG AAGAAAAAGCAGAAGAACACATGGATCTGAGGCTAAAGCATTGCTTAGCCTATGTTCTACATACATCAGG GACTACAGGGATACCGAAGATTGTCAGAGTGCCTCATAAGTGTATAGTACCAAATATCCAGCATTTTCGG GTACTTTTTGACATCACACAAGAAGATGTTTTGTTTCTGGCTTCACCTCTGACCTTCGATCCTTCTGTTG TGGAAATATTTCTTGCTCTATCAAGTGGTGCCTCTCTGCTTATTGTACCAACTTCCGTCAAGTTGCTCCC ATCAAAATTAGCCAGCGTTCTCTTTTCCCATCATAGAGTGACTGTTTTGCAGGCAACACCAACATTGCTT AGAAGATTTGGATCTCAGCTTATCAAGTCAACTGTTTTGTCAGCCACTACTTCTCTTCGAGTATTAGCCC TTGGTGGTGAAGCGTTTCCATCATTGACAGTTCTCAGAAGCTGGAGAGGAGAAGGCAATAAAACACAAAT ATTTAATGTTTATGGTATCACAGAGGTATCAAGTTGGGCGACCATTTATAGGATTCCAGAGAAGACTCTT AACTCTACTCTCAAATGTGAATTGCCTGTACAACTGGGATTTCCACTTCTTGGAACAGTAGTTGAAGTCA GAGATACTAATGGCTTCACAATTCAGGAAGGCAGTGGCCAAGTATTTTTAGGTGGCAGAAACAGAGTGTG TTTTCTTGATGATGAAGTGACAGTACCACTTGGCACAATGCGAGCTACAGGAGACTTTGTGACTGTGAAA GATGGAGAGATTTTTTTTTTGGGACGAAAAGACAGTCAGATCAAACGTCATGGCAAACGTCTTAACATTG AACTTGTGCAACAGGTTGCTGAAGAGCTTCAGCAAGTGGAGTCTTGTGCAGTTACATGGTATAATCAGGA AAAATTAATTCTCTTCATGGTGTCTAAAGATGCTTCAGTAAAAGAATACATCTTTAAAGAACTGCAGAAA TATCTTCCAAGTCATGCAGTCCCGGATGAGCTTGTATTGATCGACTCTCTACCATTTACATCCCACGGCA AAATTGATGTTTCTGAGTTAAACAAGATATATTTAAACTACATAAACTTGAAGTCTGAGAATAAGCTCAG TGGGAAAGAGGACCTTTGGGAAAAATTACAGTATTTGTGGAAGTCTACTCTGAATCTCCCAGAAGATCTT TTGAGGGTTCCTGATGAGTCACTCTTCTTAAATAGTGGTGGAGATTCCTTAAAGTCCATCCGGCTCCTCA GTGAGATTGAAAAACTTGTTGGTACATCAGTACCTGGGCTTCTGGAAATTATTCTCAGCAGTTCCATTTT AGAGATTTATAATCACATCCTTCAAACAGTGGTTCCAGATGAAGATGTGACATTCAGGAAGAGTTGTGCC ACAAAAAGGAAACTCAGCGACATTAATCAAGAGGAAGCCAGTGGAACATCTTTACATCAGAAAGCCATCA TGACTTTCACTTGCCACAATGAGATTAATGCTTTTGTTGTACTGAGCAGAGGGAGTCAAATTTTGTCTCT GAATTCCACTAGGTTTTTAACAAAGTTAGGACATTGCTCTTCAGCCTGTCCTTCTGACTCAGTTTCACAG ACCAACATTCAAAATTTGAAAGGCTTAAATTCTCCAGTTCTTATTGGGAAGTCAAAAGATCCATCCTGTG TTGCAAAAGTTTCTGAAGAGGGGAAACCTGCGATAGGGACTCAGAAAATGGAGTTACATGTGAGGTGGAG GTCAGACACAGGCAAATGTGTAGATGCTTCACCGCTGGTTGTAATACCCACTTTTGATAAGTCATCTACA ACTGTGTACATTGGTTCCCATTCTCATAGAATGAAGGCAGTTGACTTTTACTCTGGGAAGGTAAAATGGG AACAGATTTTGGGAGATCGAATTGAATCCTCAGCATGTGTATCTAAGTGTGGAAACTTTATTGTGGTGGG CTGTTATAATGGATTAGTTTATGTTCTGAAAAGTAATAGTGGAGAAAAATACTGGATGTTTACTACTGAA GATGCTGTCAAAAGCTCGGCAACCATGGATCCAACCACAGGACTCATTTACATTGGATCTCATGACCAGC ACGCATATGCTTTAGATATTTATAGAAAGAAGTGTGTTTGGAAGTCAAAATGTGGAGGAACTGTCTTTTC CTCTCCGTGTTTGAACCTGATTCCACATCATTTGTATTTTGCTACATTGGGAGGACTTTTACTGGCTGTA AATCCTGCTACTGGGAACGTTATTTGGAAACATTCCTGTGGAAAACCACTCTTCTCTTCCCCACAATGTT GCTCACAGTATATTTGTATTGGCTGTGTAGATGGGAATTTACTCTGCTTTACTCACTTTGGAGAACAGGT TTGGCAGTTCTCTACCAGTGGACCAATCTTTTCATCCCCGTGTACCTCACCATCAGAGCAAAAAATATTT TTTGGTTCCCATGATTGCTTTATCTACTGTTGTAACATGAAAGGTCACCTGCAGTGGAAATTTGAAACTA CTTCAAGGGTCTATGCAACACCGTTTGCTTTCCATAACTACAATGGCAGCAATGAAATGTTGCTGGCAGC AGCATCTACTGATGGGAAAGTGTGGATCTTGGAATCTCAGAGTGGACAATTGCAAAGTGTTTATGAACTT CCTGGAGAAGTCTTCTCTTCTCCTGTGGTCCTGGAATCAATGCTCATTATTGGGTGTAGAGATAATTATG TTTATTGTCTGGATTTATTGGGTGGCAATCAAAAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286669
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286669.1, NP_001273598.1
RefSeq Size	3493 bp
RefSeq ORF	2838 bp
Locus ID	132949
UniProt ID	Q4L235
Protein Pathways	Lysine biosynthesis, Lysine degradation, Metabolic pathways
Gene Summary	This gene encodes a member of the non-ribosome peptide syntesase (NRPS) enzyme family. The encoded protein contains an AMP-binding domain, PP-binding (phosphopantetheine, or pantetheine 4'phosphate-binding) domain and the Pyrrolo-quinoline quinon (PQQ) binding domain. The protein is expressed in several adult tissues. [provided by RefSeq, Apr 2016] Transcript Variant: This variant (3) lacks an alternate exon in the 5' end and uses a downstream start codon compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1.

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