NDST1 (NM_001301063) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSST; MRT46; NST1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001301063, the custom clone sequence may differ by one or more nucleotides ATGCCTGCCCTGGCATGCCTCCGGAGGCTGTGTCGGCACGTGTCCCCGCAGGCTGTCCTTTTCCTGCTGT TCATCTTCTGCCTGTTCAGCGTTTTCATCTCGGCCTACTACCTATATGGCTGGAAGCGAGGCCTGGAGCC CTCGGCGGATGCCCCCGAGCCTGACTGCGGGGACCCGCCGCCTGTGGCCCCCAGTCGCCTGCTGCCACTC AAGCCTGTGCAGGCAGCCACCCCTTCCCGCACAGACCCGTTGGTGCTGGTCTTTGTGGAGAGCCTCTACT CGCAACTGGGCCAGGAGGTGGTGGCCATCCTGGAGTCCAGCCGCTTCAAATACCGCACAGAGATTGCGCC GGGCAAGGGTGACATGCCCACGCTCACTGACAAGGGCCGTGGCCGCTTCGCCCTCATCATCTATGAGAAC ATCCTCAAGTATGTCAACCTGGACGCCTGGAACCGGGAGCTGCTGGACAAGTACTGTGTGGCCTACGGCG TGGGCATCATTGGCTTCTTCAAGGCCAATGAGAACAGCCTGCTGAGTGCGCAGCTCAAGGGCTTCCCCCT GTTCCTGCACTCAAACCTGGGCCTGAAGGACTGCAGCATCAACCCCAAGTCCCCGCTGCTCTACGTGACG CGACCTAGCGAGGTGGAGAAAGGTGTGCTCCCCGGCGAGGACTGGACGGTTTTCCAGTCAAATCACTCCA CCTATGAGCCAGTGCTGCTGGCCAAGACGCGCTCGTCTGAGTCCATCCCACACCTGGGCGCAGACGCCGG CCTGCATGCTGCACTGCACGCCACTGTGGTCCAGGACCTGGGCCTGCACGACGGCATCCAGCGCGTGCTG TTTGGCAACAACCTGAACTTCTGGCTGCACAAGCTTGTCTTCGTGGATGCCGTGGCCTTCCTCACGGGGA AGCGCCTCTCCCTGCCATTGGACCGCTACATCCTGGTGGACATTGATGACATCTTCGTGGGCAAGGAGGG CACACGCATGAAGGTGGAGGACGTGAAGGCCCTGTTTGACACACAGAACGAACTACGCGCACACATCCCA AACTTCACCTTCAACCTGGGCTACTCAGGGAAATTCTTCCACACAGGTACCAATGCTGAGGACGCTGGGG ATGATCTGCTGCTGTCGTATGTGAAGGAGTTCTGGTGGTTCCCCCACATGTGGAGCCACATGCAGCCCCA CCTTTTCCACAACCAGTCCGTGTTGGCCGAGCAGATGGCCTTGAACAAGAAGTTCGCTGTCGAGCATGGC ATTCCCACAGACATGGGGTATGCAGTGGCGCCCCACCACTCGGGCGTGTACCCCGTGCACGTGCAGCTGT ACGAGGCTTGGAAGCAGGTGTGGAGCATCCGCGTGACCAGCACGGAGGAGTACCCCCACCTGAAGCCAGC CCGCTACCGCCGTGGCTTCATCCACAATGGCATCATGGTTCTCCCACGGCAGACCTGCGGCCTCTTCACA CACACCATCTTCTACAACGAGTACCCTGGCGGCTCCAGTGAGCTGGACAAGATCATCAACGGGGGCGAGC TCTTCCTCACCGTGCTCCTCAATCCTATCAGCATCTTCATGACGCACCTGTCCAACTATGGGAATGACCG CCTGGGCCTGTACACCTTCAAGCACCTGGTGCGCTTCCTGCACTCCTGGACGAACCTCCGGCTGCAGACA CTGCCCCCTGTGCAGTTGGCGCAGAAGTACTTCCAGATCTTCTCCGAGGAGAAGGACCCGCTCTGGCAGG ACCCCTGCGAGGACAAACGTCACAAAGACATCTGGTCCAAGGAGAAGACGTGTGACCGCTTCCCAAAGCT CCTCATCATCGGCCCCCAGAAAACAGGCACCACTGCCCTCTACCTGTTCCTGGGCATGCACCCTGACCTA AGCAGCAACTACCCCAGCTCTGAGACCTTTGAGGAGATCCAGTTTTTTAATGGCCACAACTATCACAAAG GCATCGACTGGTACATGGAGTTCTTCCCCATCCCTTCCAACACCACCTCCGACTTCTACTTTGAGAAAAG CGCCAACTACTTTGATTCAGAAGTGGCGCCCCGGCGGGCAGCAGCCCTCTTGCCCAAAGCCAAGGTCCTG ACCATCCTCATCAACCCCGCGGACCGGGCCTATTCCTGGTACCAGATTCTGGTCTTGGATGGCAAACTGC TTCGCACAGAACCTGCCAAAGTGATGGACATGGTGCAGAAGTTCCTTGGGGTGACCAACACCATTGACTA CCACAAAACCTTGGCGTTTGATCCAAAGAAAGGATTTTGGTGCCAACTGCTTGAAGGAGGAAAAACCAAG TGTCTGGGCAAAAGCAAGGGCCGGAAATATCCCGAGATGGACTTGGATTCCCGAGCCTTCCTGAAGGACT ATTACCGGGACCACAACATCGAGCTCTCCAAGCTGCTGTATAAGATGGGCCAGACACTTCCCACTTGGCT ACGAGAGGACCTCCAGAACACCAGGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001301063
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001301063.1, NP_001287992.1
RefSeq Size	7970 bp
RefSeq ORF	2478 bp
Locus ID	3340
UniProt ID	P52848
Protein Families	Transmembrane
Protein Pathways	Heparan sulfate biosynthesis, Metabolic pathways
Gene Summary	This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. The encoded protein catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to nitrogen of glucosamine in heparan sulfate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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