EPB41L3 (NM_001281535) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	4.1B; DAL-1; DAL1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001281535, the custom clone sequence may differ by one or more nucleotides ATGCAGTGCAAAGTGATACTTCTCGATGGATCAGAATATACCTGTGATGTAGAGAAACGCTCCAGAGGAC AAGTGCTGTTTGATAAAGTGTGTGAACACTTGAACTTGCTAGAGAAAGACTACTTTGGGCTTACGTATCG AGATGCTGAAAACCAGAAGAATTGGTTGGACCCTGCTAAGGAAATAAAAAAACAGGTTCGAAGTGGTGCT TGGCACTTTTCATTTAATGTGAAATTTTATCCACCAGACCCTGCCCAACTATCTGAAGATATCACCAGGT ACTACCTCTGCTTGCAGTTGCGAGATGACATCGTGTCCGGAAGGCTGCCCTGCTCCTTTGTTACCCTGGC CTTGCTGGGCTCCTACACTGTCCAGTCAGAGCTCGGAGACTATGACCCAGATGAATGTGGGAGCGATTAC ATTAGTGAGTTCCGCTTTGCACCAAACCACACTAAAGAACTGGAAGACAAAGTGATCGAGCTGCACAAGA GCCACAGAGGAATGACGCCAGCAGAAGCAGAGATGCATTTCTTGGAAAATGCCAAAAAATTATCAATGTA TGGGGTAGATTTACATCATGCTAAGGACTCAGAAGGGGTAGAAATTATGTTAGGAGTTTGTGCAAGTGGT CTGTTGATATATCGCGACCGGCTGCGAATAAACAGATTTGCCTGGCCCAAGGTTCTAAAGATTTCATACA AACGGAACAACTTTTACATTAAGATCCGGCCGGGAGAGTTTGAACAATTTGAAAGCACCATTGGGTTTAA GCTGCCAAACCATCGAGCTGCCAAGCGTTTATGGAAAGTATGTGTTGAGCATCATACATTTTTCAGACTA CTGTTACCAGAAGCACCTCCCAAGAAATTCCTAACCTTGGGTTCCAAGTTTCGTTATAGTGGCAGGACAC AAGCGCAAACGAGAAGAGCCAGTGCGTTGATAGATCGCCCAGCACCTTACTTTGAACGCTCATCCAGCAA ACGTTATACCATGTCTCGCAGCTTGGATGGAGCATCAGTGAATGAAAACCATGAAATATACATGAAGGAT TCTATGTCTGCTGCAGAGGTTGGTACTGGCCAGTACGCCACAACAAAAGGCATCTCTCAGACCAACTTGA TCACCACTGTGACTCCGGAGAAGAAGGCTGAGGAGGAGCGGGACGAGGAAGAGGACAAACGGAGGAAGGG GGAAGAAGTCACGCCCATCTCGGCCATCCGGCACGAGGGAAAGACTGACAGTGAGCGCACGGACACCGCA GCCGACGGGGAGACCACTGCCACTGAGGAGCTAGAAAAAACTCAAGATGACCTGATGAAACATCAAACCA ACATTAGCGAGCTGAAAAGAACCTTCTTAGAAACCTCAACAGACACTGCCGTAACGAATGAATGGGAGAA GAGGCTTTCCACCTCCCCCGTGCGACTGGCCGCCAGGCAGGAGGATGCCCCCATGATCGAACCACTTGTC CCTGAAGAGAAAATGGAAACCAAGACGGAGTCCAGTGGAATAGAGACGGAACCCACCGTGCACCACCTGC CGCTTAGCACTGAGAAGGTGGTGCAGGAGACCGTGTTGGTGGAGGAGCGGCGTGTGGTGCACGCGAGTGG GGATGCTTCTTACTCGGCGGGAGACAGCGGGGATGCTGCAGCACAGCCCGCATTCACAGGCATTAAAGGG AAAGAGGGCTCTGCCTTGACGGAGGGGGCTAAAGAGGAAGGAGGGGAGGAGGTCGCTAAAGCTGTCCTGG AACAGGAAGAGACAGCCGCTGCTTCCCGTGAGCGACAAGAGGAGCAGAGTGCAGCCATCCACATTTCAGA AACTTTGGAACAAAAACCTCATTTTGAGTCCTCAACGGTGAAGACGGAAACCATCAGTTTTGGCAGTGTT TCACCGGGAGGAGTAAAGCTAGAAATTTCCACGAAGGAAGTGCCAGTAGTTCACACCGAAACCAAAACCA TCACATATGAATCATCACAGGTCGATCCAGGCACAGATCTGGAGCCAGGCGTGCTGATGAGTGCACAGAC GATCACATCTGAAACCACCAGTACCACCACCACTACGCACATCACCAAAACTGTGAAAGGGGGCATTTCA GAGACAAGAATTGAGAAGCGAATAGTCATCACGGGGGATGCAGACATTGACCATGACCAGGCGCTGGCTC AGGCAATTAAAGAGGCCAAAGAGCAGCACCCTGACATGTCAGTGACCAAAGTAGTGGTCCATAAAGAGAC AGAGATCACACCAGAAGATGGAGAGGATTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001281535
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001281535.1, NP_001268464.1
RefSeq Size	3912 bp
RefSeq ORF	2271 bp
Locus ID	23136
UniProt ID	Q9Y2J2
Protein Pathways	Tight junction
Gene Summary	Tumor suppressor that inhibits cell proliferation and promotes apoptosis. Modulates the activity of protein arginine N-methyltransferases, including PRMT3 and PRMT5.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternate promoter, differs in the 5' UTR, initiates translation from an in-frame downstream start codon, uses an alternate in-frame acceptor splice site at an internal exon, and lacks 3 non-consecutive in-frame coding exons compared to variant 1. The resulting isoform (4) is shorter compared to isoform 1.

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