EOMES (NM_001278182) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TBR2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278182, the custom clone sequence may differ by one or more nucleotides ATGCAGTTAGGGGAGCAGCTCTTGGTGAGCTCAGTGAACCTGCCTGGCGCGCACTTCTACCCGCTGGAGA GTGCGCGAGGCGGCAGCGGCGGGAGCGCTGGCCACCTCCCCAGCGCGGCCCCCTCTCCTCAGAAGTTGGA CTTAGACAAAGCGTCCAAGAAGTTTTCCGGCAGTCTCTCCTGCGAGGCGGTGAGCGGGGAGCCCGCAGCC GCCAGCGCAGGGGCCCCCGCGGCCATGCTTAGTGACACCGACGCCGGGGACGCATTTGCCAGCGCTGCGG CAGTGGCCAAGCCGGGGCCCCCGGACGGCCGCAAGGGCTCCCCCTGCGGGGAGGAGGAGCTGCCCTCCGC CGCTGCAGCCGCCGCCGCCGCCGCCGCCGCGGCTGCGGCCACTGCGCGCTACTCCATGGACAGCCTGAGC TCCGAGCGGTACTACCTCCAGTCCCCCGGTCCTCAGGGGTCGGAGCTGGCTGCGCCCTGCTCACTCTTCC CGTACCAGGCGGCGGCTGGGGCGCCCCACGGACCTGTGTACCCGGCTCCTAACGGGGCGCGCTACCCCTA CGGCTCCATGCTGCCCCCCGGCGGCTTCCCCGCGGCTGTGTGCCCACCCGGGAGGGCGCAGTTCGGCCCA GGAGCCGGTGCGGGCAGTGGCGCGGGCGGTAGCAGCGGCGGGGGCGGCGGCCCGGGCACCTATCAGTACA GCCAGGGGGCTCCGCTCTACGGGCCGTACCCTGGAGCCGCAGCGGCGGGATCTTGCGGAGGACTGGGGGG CCTGGGGGTTCCAGGTTCTGGCTTCCGTGCCCACGTCTACCTGTGCAACCGGCCTCTGTGGCTCAAATTC CACCGCCACCAAACTGAGATGATCATTACGAAACAGGGCAGGCGCATGTTTCCTTTCTTGAGCTTCAACA TAAACGGACTCAATCCCACTGCCCACTACAATGTGTTCGTAGAGGTGGTGCTGGCGGACCCCAACCACTG GCGCTTCCAGGGGGGCAAATGGGTGACCTGTGGCAAAGCCGACAATAACATGCAGGGCAACAAAATGTAT GTTCACCCAGAGTCTCCTAATACTGGTTCCCACTGGATGAGACAGGAGATTTCATTCGGGAAATTAAAAC TCACCAATAACAAAGGCGCAAATAACAACAACACCCAGATGATAGTCTTACAATCCTTACACAAATACCA ACCCCGACTGCATATTGTTGAAGTTACAGAGGATGGCGTGGAGGACTTGAATGAGCCCTCAAAGACCCAG ACTTTTACCTTCTCAGAAACGCAATTCATTGCAGTGACTGCCTACCAAAACACCGATATTACTCAACTAA AGATTGATCATAACCCCTTTGCAAAAGGCTTCAGAGACAACTATGATTCCATGTACACCGCTTCAGAAAA TGACAGGTTAACTCCATCTCCCACGGATTCTCCTAGATCCCATCAGATTGTCCCTGGAGGTCGGTACGGC GTTCAATCCTTCTTCCCGGAGCCCTTTGTCAACACTTTACCTCAAGCCCGCTATTATAATGGCGAGAGAA CCGTGCCACAGACCAACGGCCTCCTTTCACCCCAACAGAGCGAAGAGGTGGCCAACCCTCCCCAGCGGTG GCTTGTCACGCCTGTCCAGCAACCTGGGACCAACAAACTAGACATCAGTTCCTATGAATCTGAATATACT TCTAGCACATTGCTCCCATATGGCATTAAATCCTTGCCCCTTCAGACATCCCATGCCCTGGGGTATTACC CAGACCCAACCTTTCCTGCAATGGCAGGGTGGGGAGGTCGAGGTTCTTACCAGAGGAAGATGGCAGCTGG ACTACCATGGACCTCCAGAACAAGCCCCACTGTGTTCTCTGAAGATCAGCTCTCCAAGGAGAAAGTGAAA GAGGAAATTGGCTCTTCTTGGATAGAGACACCCCCTTCCATCAAATCTCTAGATTCCAATGATTCAGGAG TATACACCAGTGCTTGTAAGCGAAGGCGGCTGTCTCCTAGCAACTCCAGTAATGAAAATTCACCCTCCAT AAAGTGTGAGGACATTAATGCTGAAGAGTATAGTAAAGACACCTCAAAAGGCATGGGAGGGTATTATGCT TTTTACACAACTCCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001278182
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278182.1, NP_001265111.1
RefSeq Size	3674 bp
RefSeq ORF	2118 bp
Locus ID	8320
UniProt ID	O95936
Protein Families	Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
Gene Summary	This gene belongs to the TBR1 (T-box brain protein 1) sub-family of T-box genes that share the common DNA-binding T-box domain. The encoded protein is a transcription factor which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. The protein may also be necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. A similar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).

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