STT3A (NM_001278504) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ITM1; STT3-A; TMC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336981 representing NM_001278504. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATCACCTCTGCTGCAATCTACCATGTACTCCATTTTTTCCACATCACCATCGACATTCGGAATGTC TGTGTGTTCCTGGCCCCTCTCTTCTCCTCCTTCACCACCATCGTCACGTACCACCTTACCAAAGAGCTC AAGGATGCAGGGGCTGGGCTTCTTGCTGCTGCCATGATTGCTGTAGTTCCTGGATATATCTCCCGATCT GTGGCTGGCTCCTATGATAATGAAGGGATTGCCATCTTTTGCATGCTACTCACCTACTACATGTGGATC AAGGCAGTAAAGACTGGTTCCATCTGTTGGGCAGCTAAGTGTGCCCTTGCTTATTTCTACATGGTCTCG TCATGGGGAGGTTATGTGTTCCTGATCAACTTAATTCCTCTCCACGTCCTCGTGCTGATGCTCACAGGC CGTTTCTCTCACCGGATCTATGTGGCCTACTGTACTGTTTACTGCCTGGGCACTATACTTTCTATGCAG ATCTCCTTTGTGGGTTTCCAGCCTGTCCTTTCATCAGAGCACATGGCAGCCTTTGGGGTCTTTGGTCTC TGCCAGATCCATGCCTTTGTGGATTACCTGCGCAGCAAGTTGAATCCACAACAATTTGAAGTTCTTTTC CGGAGCGTCATCTCTCTGGTAGGCTTTGTCCTTCTCACCGTGGGAGCTCTCCTCATGCTGACAGGAAAA ATATCTCCCTGGACGGGGCGTTTCTACTCGCTGCTGGATCCCTCTTATGCTAAGAACAACATCCCCATC ATTGCTTCTGTGTCTGAGCATCAGCCCACAACCTGGTCCTCATACTATTTTGACCTGCAGCTCCTCGTC TTCATGTTTCCAGTTGGCCTCTATTACTGCTTTAGCAACCTGTCTGATGCCCGGATTTTTATCATCATG TATGGTGTGACCAGCATGTACTTTTCAGCTGTAATGGTGCGTCTAATGCTAGTGTTGGCACCTGTTATG TGCATTCTCTCTGGCATTGGAGTCTCCCAGGTGCTGTCCACATACATGAAGAATCTGGACATAAGTCGT CCAGACAAGAAGAGCAAGAAGCAACAGGATTCCACCTACCCTATTAAGAATGAAGTGGCAAGTGGGATG ATACTGGTCATGGCTTTCTTTCTCATCACCTACACCTTTCATTCAACCTGGGTGACCAGTGAGGCCTAC TCTTCTCCGTCCATTGTACTATCTGCCCGTGGTGGGGATGGCAGTAGGATCATATTTGATGACTTCCGA GAAGCATATTATTGGCTTCGTCATAATACTCCAGAGGATGCGAAGGTCATGTCCTGGTGGGATTATGGC TATCAGATTACAGCTATGGCAAACCGAACAATTTTAGTGGACAATAACACATGGAATAATACCCATATT TCTCGAGTAGGGCAGGCAATGGCGTCCACAGAGGAAAAAGCCTATGAGATCATGAGGGAGCTCGATGTC AGCTATGTGCTGGTCATTTTTGGAGGCCTCACTGGGTATTCCTCTGATGATATCAACAAGTTTCTTTGG ATGGTCCGGATTGGAGGGAGCACAGATACAGGCAAACATATCAAGGAGAATGACTATTATACTCCAACT GGGGAGTTCCGTGTGGACCGTGAAGGTTCTCCAGTGCTGCTCAACTGCCTCATGTACAAGATGTGTTAC TATCGCTTTGGACAGGTTTACACAGAAGCCAAGCGTCCTCCAGGCTTTGACCGTGTCCGAAATGCTGAG ATTGGGAATAAAGACTTTGAGCTTGATGTCCTGGAGGAAGCATATACCACAGAACATTGGCTGGTCAGG ATATACAAGGTAAAGGACCTGGATAATCGAGGCTTGTCAAGGACATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278504
Insert Size	1842 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278504.1
RefSeq Size	4121 bp
RefSeq ORF	1842 bp
Locus ID	3703
UniProt ID	P46977
Protein Families	Transmembrane
Protein Pathways	Metabolic pathways, N-Glycan biosynthesis
MW	69.6 kDa
Gene Summary	The protein encoded by this gene is a catalytic subunit of the N-oligosaccharyltransferase (OST) complex, which functions in the endoplasmic reticulum to transfer glycan chains to asparagine residues of target proteins. A separate complex containing a similar catalytic subunit with an overlapping function also exists. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (3) differs in the 5' UTR and lacks the exon containing the translation start codon compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (b) with a shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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