ETS2 (NM_001256295) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ETS2IT1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336970 representing NM_001256295. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGTCGGCTCAATTTCAGGGCCTTATTACCCAAGCCCGGCTGCCCTTCGGTGCCACCAGCACCACT GCTCCGTCGCTGCGGAATTCCAAAGGCAGGTTTGGCGTTAGGGCCTTGGCCCCAGAGAGGACGCCGAGC GCTCCACGGAAAGTCTCCGCCCGGCTCCCAGGGCGCACACTCGCGCGCACGTGGGGCCGAGGCCCTGCT CCCGGGGCCTCAGGGCCAGCCGGCGAGGGACCCAGCCGAGTGACAGCAGGAGGCGGAGGGAAGCTCAGA GCTCCCGGAGCCGCCCGGCCAGCGTCCGGCCTCCCTGATCGTCTCTGGCCGGCGCCCTCGCCCTCGCCC GGCGCGCACCGAGCAGCCGCGGGCGCCGAGCAGCCACCGTCCCGACCAAGCGCCGGCCCTGCCCGCAGC GGCAGGATGAATGATTTCGGAATCAAGAATATGGACCAGGTAGCCCCTGTGGCTAACAGTTACAGAGGG ACACTCAAGCGCCAGCCAGCCTTTGACACCTTTGATGGGTCCCTGTTTGCTGTTTTTCCTTCTCTAAAT GAAGAGCAAACACTGCAAGAAGTGCCAACAGGCTTGGATTCCATTTCTCATGACTCCGCCAACTGTGAA TTGCCTTTGTTAACCCCGTGCAGCAAGGCTGTGATGAGTCAAGCCTTAAAAGCTACCTTCAGTGGCTTC AAAAAGGAACAGCGGCGCCTGGGCATTCCAAAGAACCCCTGGCTGTGGAGTGAGCAACAGGTATGCCAG TGGCTTCTCTGGGCCACCAATGAGTTCAGTCTGGTGAACGTGAATCTGCAGAGGTTCGGCATGAATGGC CAGATGCTGTGTAACCTTGGCAAGGAACGCTTTCTGGAGCTGGCACCTGACTTTGTGGGTGACATTCTC TGGGAACATCTGGAGCAAATGATCAAAGAAAACCAAGAAAAGACAGAAGATCAATATGAAGAAAATTCA CACCTCACCTCCGTTCCTCATTGGATTAACAGCAATACATTAGGTTTTGGCACAGAGCAGGCGCCCTAT GGAATGCAGACACAGAATTACCCCAAAGGCGGCCTCCTGGACAGCATGTGTCCGGCCTCCACACCCAGC GTACTCAGCTCTGAGCAGGAGTTTCAGATGTTCCCCAAGTCTCGGCTCAGCTCCGTCAGCGTCACCTAC TGCTCTGTCAGTCAGGACTTCCCAGGCAGCAACTTGAATTTGCTCACCAACAATTCTGGGACTCCCAAA GACCACGACTCCCCTGAGAACGGTGCGGACAGCTTCGAGAGCTCAGACTCCCTCCTCCAGTCCTGGAAC AGCCAGTCGTCCTTGCTGGATGTGCAACGGGTTCCTTCCTTCGAGAGCTTCGAAGATGACTGCAGCCAG TCTCTCTGCCTCAATAAGCCAACCATGTCTTTCAAGGATTACATCCAAGAGAGGAGTGACCCAGTGGAG CAAGGCAAACCAGTTATACCTGCAGCTGTGCTGGCCGGCTTCACAGGAAGTGGACCTATTCAGCTGTGG CAGTTTCTCCTGGAGCTGCTATCAGACAAATCCTGCCAGTCATTCATCAGCTGGACTGGAGACGGATGG GAGTTTAAGCTCGCCGACCCCGATGAGGTGGCCCGCCGGTGGGGAAAGAGGAAAAATAAGCCCAAGATG AACTACGAGAAGCTGAGCCGGGGCTTACGCTACTATTACGACAAGAACATCATCCACAAGACGTCGGGG AAGCGCTACGTGTACCGCTTCGTGTGCGACCTCCAGAACTTGCTGGGGTTCACGCCCGAGGAACTGCAC GCCATCCTGGGCGTCCAGCCCGACACGGAGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001256295
Insert Size	1830 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256295.1
RefSeq Size	3954 bp
RefSeq ORF	1830 bp
Locus ID	2114
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Dorso-ventral axis formation
MW	66.9 kDa
Gene Summary	This gene encodes a transcription factor which regulates genes involved in development and apoptosis. The encoded protein is also a protooncogene and shown to be involved in regulation of telomerase. A pseudogene of this gene is located on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (2) differs in the 5' UTR and coding region, and initiates translation at an alternate start codon, compared to variant 1. The resulting protein (isoform 2) has a longer N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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