Cip4 (TRIP10) (NM_001288963) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CIP4; HSTP; STOT; STP; TRIP-10
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336914 representing NM_001288963. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATTGGGGCACTGAGCTGTGGGATCAGTTCGAGGTGCTCGAGCGCCACACGCAGTGGGGGCTGGAC CTGTTGGACAGATATGTAAAGTTCGTGAAAGAACGCACCGAAGTGGAACAGGCTTACGCCAAACAACTG CGGAGCCTGGTGAAAAAATATCTGCCCAAGAGACCTGCCAAGGATGATCCTGAGTCCAAATTCAGCCAG CAACAGTCCTTCGTACAGATTCTCCAGGAGGTGAATGACTTTGCAGGCCAGCGGGAGCTGGTGGCTGAG AACCTCAGTGTCCGTGTATGTCTTGAGCTGACCAAGTACTCACAAGAGATGAAACAGGAGAGGAAGATG CACTTCCAAGAAGGGCGGCGGGCCCAGCAGCAGCTGGAAAATGGCTTTAAACAGCTGGAGAATAGTAAG CGTAAATTTGAGCGGGACTGCCGGGAGGCAGAGAAGGCAGCCCAGACTGCTGAACGGCTAGACCAGGAT ATCAACGCCACCAAGGCTGATGTGGAGAAGGCCAAGCAGCAAGCCCACCTTCGGAGTCACATGGCCGAA GAAAGCAAAAACGAATATGCGGCTCAACTGCAGCGCTTCAACCGAGACCAAGCCCACTTCTATTTTTCA CAGATGCCCCAGATATTCGATAAGCTCCAAGACATGGATGAACGCAGGGCCACCCGCCTGGGTGCCGGG TATGGGCTCCTGTCGGAGGCCGAGCTGGAGGTGGTGCCCATAATAGCCAAGTGCTTGGAGGGCATGAAG GTGGCTGCAAATGCTGTGGATCCCAAGAACGACTCCCACGTCCTTATAGAGCTGCACAAGTCAGGTTTT GCCCGCCCGGGCGACGTGGAATTCGAGGACTTCAGCCAGCCCATGAACCGTGCACCCTCCGACAGCAGT CTGGGCACCCCCTCGGATGGACGGCCTGAACTCCGAGGCCCGGGTCGCAGCCGCACCAAGCGCTGGCCT TTTGGCAAGAAGAACAAGACAGTGGTGACCGAGGATTTTAGCCACTTGCCCCCAGAGCAGCAGCGAAAA CGGCTTCAACAGCAGTTGGAAGAACGCAGTCGTGAACTTCAGAAGGAGGTTGACCAGAGGGAAGCCCTA AAGAAAATGAAGGATGTCTATGAGAAGACACCTCAGATGGGGGACCCCGCCAGCTTGGAGCCCCAGATC GCTGAAACCCTGAGCAACATTGAACGGCTGAAATTGGAAGTGCAGAAGTATGAGGCGTGGCTGGCAGAA GCTGAAAGTCGAGTCCTTAGCAACCGGGGAGACAGCCTGAGCCGGCACGCCCGGCCTCCCGACCCCCCC GCTAGCGCCCCGCCAGACAGCAGCAGCAACAGCGCATCACAGGACACCAAGGAGAGCTCTGAAGAGCCT CCCTCAGAAGAGAGCCAGGACACCCCCATTTACACGGAGTTTGATGAGGATTTCGAGGAGGAACCCACA TCCCCCATAGGTCACTGTGTGGCCATCTACCACTTTGAAGATCTGGGCCCACCCCCACCCCCATCACAG GGTCCAGCGAGGGCACTATCTCTATGGCCGAGGGTGAAGACCTCAGTCTTATGGAAGAAGACAAAGGGG ACGGCTGGACCCGGGTCAGGCGGAAAGAGGGAGGCGAGGGCTACGTGCCCACCTCCTACCTCCGAGTCA CGCTCAATTGAACCCTGCCAGAGACGGGAAGAGGGGGGCTGTCGGCTGCTGCTTCTGGGCCACGGGGAG CCCCAGGACCTATGCACTTTATTTCTGACCCCGTGGCTTCGGCTGAGACCTGTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001288963
Insert Size	1782 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288963.1
RefSeq Size	2062 bp
RefSeq ORF	1782 bp
Locus ID	9322
UniProt ID	Q15642
Protein Families	Druggable Genome
Protein Pathways	Insulin signaling pathway
MW	67.7 kDa
Gene Summary	Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling (By similarity). Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. May be required for the lysosomal retention of FASLG/FASL.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an in-frame exon in the central coding region, and uses an alternate splice site in the 3' coding region, which leads to a frameshift, compared to variant 1. The encoded isoform (3) lacks an internal segment and has a longer and distinct C-terminus, compared to isoform 1.

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