MAN1C1 (NM_001289010) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HMIC; MAN1A3; MAN1C; pp6318
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336907 representing NM_001289010. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTCATGAGGAAAGTGCCCGGCTTCGTCCCGGCCTCCCCGTGGGGGCTGCGGCTGCCGCAGAAGTTC CTCTTCCTCCTCTTCCTCTCGGGCCTGGTCACCCTGTGCTTCGGGGCCCTCTTCCTGCTGCCCCACTCC TCTCGCCTCAAGCGCCTCTTCCTGGCCCCCCGGACCCAGCAGCCTGGTCTGGAAGTGGTGGCTGAAATC GCCGGCCATGCCCCGGCCCGCGAGCAGGAGCCGCCTCCCAACCCGGCCCCCGCCGCGCCGGCCCCGGGC GAGGATGACCCCAGCAGCTGGGCCAGTCCCCGCCGCAGGAAAGGGGGGCTGCGGCGCACCCGCCCCACT GGACCCCGCGAGGAGGCCACGGCGGCCCGGGGCAATAGCATCCCGGCCTCCAGGCCCGGGGACGAGGGC GTCCCTTTCCGCTTTGACTTCAACGCATTCCGGAGCCGTCTCCGCCACCCGGTCCTGGGAACGAGGGCC GATGAGAGTCAGGAGCCCCAGAGCCAAGTGCGAGCCCAGCGGGAGAAAATCAAGGAGATGATGCAGTTT GCTTGGCAGAGCTATAAGCGTTATGCAATGGGGAAAAACGAACTCCGTCCACTAACAAAAGATGGCTAC GAGGGTAACATGTTCGGAGGCCTCAGCGGGGCAACAGTCATTGACTCCCTCGATACCCTCTACCTCATG GAGCTGAAGGAGGAGTTCCAGGAGGCCAAGGCCTGGGTGGGAGAGAGCTTCCACCTGAACGTGAGCGGA GAAGCATCCTTGTTTGAGGTGAACATCCGCTACATCGGGGGACTCCTCTCAGCCTTCTACCTGACAGGA GAAGAGGTGTTCCGAATAAAGGCCATCAGGCTGGGAGAGAAGCTCCTGCCGGCGTTCAACACCCCCACG GGAATCCCAAAGGGCGTGGTGAGCTTCAAAAGTGGGAACTGGGGCTGGGCCACAGCCGGCAGCAGCAGC ATCTTGGCGGAGTTTGGATCCCTGCACTTGGAATTCTTACACCTCACTGAACTCTCTGGCAACCAGGTC TTCGCTGAAAAGGTCAGGAACATCCGCAAGGTCCTCAGGAAGATCGAAAAGCCCTTTGGCCTCTACCCC AACTTCCTCAGCCCAGTGAGTGGGAACTGGGTGCAACACCATGTCTCAGTTGGAGGACTCGGGGACAGT TTTTATGAATATTTGATCAAATCCTGGTTGATGTCGGGCAAGACAGATATGGAGGCTAAAAATATGTAC TACGAAGCCTTGGAGGCGATAGAGACCTACTTGCTGAATGTCTCTCCCGGGGGGCTGACCTACATTGCC GAGTGGCGAGGGGGGATTCTGGACCACAAGATGGGGCACCTGGCCTGTTTCTCCGGGGGCATGATCGCC CTTGGCGCCGAGGATGCCAAGGAAGAAAAGAGGGCCCACTACCGAGAGCTCGCAGCCCAGATCACCAAG ACGTGTCACGAGTCATACGCCCGCTCAGACACCAAACTTGGGCCTGAGGCCTTCTGGTTTAACTCCGGC AGAGAGGCCGTGGCCACCCAGCTGAGCGAGAGCTACTACATCCTCCGGCCAGAGGTGGTGGAGAGCTAC ATGTACCTGTGGCGACAGACCCACAACCCCATCTACAGGGAGTGGGGCTGGGAGGTGGTGCTGGCCTTG GAGAAATACTGTCGGACAGAAGCCGGTTTCTCTGGGATCCAAGACGTGTACAGTAGCACCCCCAACCAC GACAACAAGCAGCAGAGCTTCTTTCTAGCGGAGACACTAAAGATTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001289010
Insert Size	1773 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289010.1
RefSeq Size	4476 bp
RefSeq ORF	1773 bp
Locus ID	57134
UniProt ID	Q9NR34
Protein Families	Transmembrane
Protein Pathways	Metabolic pathways, N-Glycan biosynthesis
MW	66.3 kDa
Gene Summary	Involved in the maturation of Asn-linked oligosaccharides. Trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce first Man(8)GlcNAc(2) then Man(6)GlcNAc and a small amount of Man(5)GlcNAc.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1. The results in a shorter protein (isoform 2) with a distinct C-terminus, compared to isoform 1.

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