STIP1 (NM_001282653) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HEL-S-94n; HOP; IEF-SSP-3521; P60; STI1; STI1L
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336588 representing NM_001282653. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCAGGTCAATGAGCTGAAGGAGAAAGGCAACAAGGCCCTGAGCGTGGGTAACATCGATGATGCC TTACAGTGCTACTCCGAAGCTATTAAGCTGGATCCCCACAACCACGTGCTGTACAGCAACCGTTCTGCT GCCTATGCCAAGAAAGGAGACTACCAGAAGGCTTATGAGGATGGCTGCAAGACTGTCGACCTAAAGCCT GACTGGGGCAAGGGCTTAAAACACGAGGCAAATAACCCTCAACTGAAAGAGGGTTTACAGAATATGGAG GCCAGGTTGGCAGAGAGAAAATTCATGAACCCTTTCAACATGCCTAATCTGTATCAGAAGTTGGAGAGT GATCCCAGGACAAGGACACTACTCAGTGATCCTACCTACCGGGAGCTGATAGAGCAGCTACGAAACAAG CCTTCTGACCTGGGCACGAAACTACAAGATCCCCGGATCATGACCACTCTCAGCGTCCTCCTTGGGGTC GATCTGGGCAGTATGGATGAGGAGGAAGAGATTGCAACACCTCCACCACCACCCCCTCCCAAAAAGGAG ACCAAGCCAGAGCCAATGGAAGAAGATCTTCCAGAGAATAAGAAGCAGGCACTGAAAGAAAAAGAGCTG GGGAACGATGCCTACAAGAAGAAAGACTTTGACACAGCCTTGAAGCATTACGACAAAGCCAAGGAGCTG GACCCCACTAACATGACTTACATTACCAATCAAGCAGCGGTATACTTTGAAAAGGGCGACTACAATAAG TGCCGGGAGCTTTGTGAGAAGGCCATTGAAGTGGGGAGAGAAAACCGAGAAGACTATCGACAGATTGCC AAAGCATATGCTCGAATTGGCAACTCCTACTTCAAAGAAGAAAAGTACAAGGATGCCATCCATTTCTAT AACAAGTCTCTGGCAGAGCACCGAACCCCAGATGTGCTCAAGAAATGCCAGCAGGCAGAGAAAATCCTG AAGGAGCAAGAGCGGCTGGCCTACATAAACCCCGACCTGGCTTTGGAGGAGAAGAACAAAGGCAACGAG TGTTTTCAGAAAGGGGACTATCCCCAGGCCATGAAGCATTATACAGAAGCCATCAAAAGGAACCCGAAA GATGCCAAATTATACAGCAATCGAGCTGCCTGCTACACCAAACTCCTGGAGTTCCAGCTGGCACTCAAG GACTGTGAGGAATGTATCCAGCTGGAGCCGACCTTCATCAAGGGTTATACACGGAAAGCCGCTGCGCTG GAAGCGATGAAGGACTACACCAAAGCCATGGATGTGTACCAGAAGGCGCTAGACCTGGACTCCAGCTGT AAGGAGGCGGCAGACGGCTACCAGCGCTGTATGATGGCGCAGTACAACCGGCACGACAGCCCCGAAGAT GTGAAGCGACGAGCCATGGCCGACCCTGAGGTGCAGCAGATCATGAGTGACCCAGCCATGCGCCTTATC CTGGAACAGATGCAGAAGGACCCCCAGGCACTCAGCGAACACTTAAAGAATCCTGTAATAGCACAGAAG ATCCAGAAGCTGATGGATGTGGGTCTGATTGCAATTCGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282653
Insert Size	1560 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282653.1
RefSeq Size	2147 bp
RefSeq ORF	1560 bp
Locus ID	10963
UniProt ID	P31948
Protein Families	Stem cell - Pluripotency
Protein Pathways	Prion diseases
MW	59.8 kDa
Gene Summary	STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009] Transcript Variant: This variant (3) uses an alternate 5' terminal exon and thus differs in the 5' UTR and 5' coding region, uses an alternate start codon, and contains an in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is shorter than isoform a.

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