DTX4 (NM_001300727) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RNF155
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336546 representing NM_001300727. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAGTGGGCATCACCATCCAGCATGCCTATGAGAAGCAGCACCCCTGGATCGACCTCACTTCCATT GGCTTTAGCTACGTAATTGACTTCAACACCATGGGCCAGATCAACCGTCAGACCCAGCGCCAACGCCGC GTCCGCCGGCGCCTCGACCTCATCTACCCCATGGTCACAGGGACCTTGCCTAAGGCTCAGTCCTGGCCA GTCAGCCCTGGGCCAGCCACCTCGCCCCCCATGTCCCCCTGCTCCTGTCCCCAGTGTGTCTTGGTGATG AGTGTTAAGGCAGCCGTGGTCAATGGCAGCACTGGGCCCCTACAGCTGCCAGTGACCCGCAAGAACATG CCGCCTCCTGGAGTGGTCAAGCTACCCCCACTGCCAGGCTCTGGGGCCAAGCCACTGGACAGCACAGGC ACCATTCGAGGCCCACTGAAGACCGCCCCATCGCAGGTGATCCGGAGACAAGCCTCCAGCATGCCCACT GGGACAACCATGGGCTCTCCTGCCAGTCCCCCAGGACCCAACAGCAAGACCGGAAGGGTGGCCCTGGCC ACCTTGAATCGTACCAACCTGCAGCGACTGGCCATTGCCCAGTCCCGGGTGCTGATCGCCTCTGGGGTC CCCACAGTCCCAGTGAAGAACCTAAATGGGTCCAGTCCTGTCAACCCTGCCTTGGCAGGAATCACTGGG ATCCTCATGAGTGCAGCGGGGCTGCCTGTGTGTCTCACCAGGCCACCAAAGCTGGTCCTACACCCACCC CCCGTCAGCAAGAGTGAAATAAAATCCATCCCAGGGGTTTCCAACACAAGCCGCAAGACCACCAAAAAA CAAGCCAAGAAAGGTAAAACCCCAGAGGAAGTGCTAAAAAAATATCTACAGAAAGTCCGGCACCCACCA GATGAGGACTGCACCATCTGTATGGAACGCCTCACGGCCCCCTCAGGCTACAAGGGCCCGCAGCCTACG GTAAAACCTGACCTGGTAGGGAAGCTGTCCAGATGCGGCCACGTCTACCACATCTACTGCTTGGTTGCC ATGTACAACAATGGGAACAAGGATGGAAGTTTGCAGTGTCCAACCTGCAAGACCATTTATGGGGTGAAG ACAGGCACCCAACCTCCAGGGAAGATGGAGTACCACCTCATCCCCCACTCCTTGCCTGGCCACCCAGAC TGCAAAACCATCCGGATCATCTACAGCATCCCCCCCGGCATTCAGGGACCGGAACACCCGAATCCTGGG AAGAGTTTCAGCGCCCGAGGCTTCCCACGACACTGTTACCTTCCGGACAGCGAGAAAGGGAGAAAAGTT CTGAAGCTGCTGCTCGTGGCCTGGGATCGCCGCCTCATTTTTGCCATTGGCACCTCCAGCACCACAGGC GAGTCAGACACCGTCATCTGGAATGAGGTCCACCACAAGACAGAGTTTGGCTCTAATCTCACTGGCCAT GGCTACCCAGATGCCAATTACCTGGATAATGTGCTGGCTGAACTGGCTGCCCAGGGCATCTCTGAGGAC AGCACTGCCCAGGAGAAGGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001300727
Insert Size	1542 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300727.1
RefSeq Size	5386 bp
RefSeq ORF	1542 bp
Locus ID	23220
UniProt ID	Q9Y2E6
Protein Families	Druggable Genome
Protein Pathways	Notch signaling pathway
MW	55.3 kDa
Gene Summary	Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations (By similarity). Functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has an alternate exon in place of the first exon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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