PLAGL1 (NM_001289045) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LOT1; ZAC; ZAC1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336273 representing NM_001289045. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCACGTTCCCCTGCCAGTTATGTGGCAAGACGTTCCTCACCCTGGAGAAGTTCACGATTCACAAT TATTCCCACTCCAGGGAGCGGCCGTACAAGTGTGTGCAGCCTGACTGTGGCAAAGCCTTTGTTTCCAGA TATAAATTGATGAGGCATATGGCTACCCATTCTCCCCAGAAATCTCACCAGTGTGCTCACTGTGAGAAG ACGTTCAACCGGAAAGACCACCTGAAAAACCACCTCCAGACCCACGACCCCAACAAAATGGCCTTTGGG TGTGAGGAGTGTGGGAAGAAGTACAACACCATGCTGGGCTATAAGAGGCACCTGGCCCTCCATGCGGCC AGCAGTGGGGACCTCACCTGTGGGGTCTGTGCCCTGGAGCTAGGGAGCACCGAGGTGCTACTGGACCAC CTCAAAGCCCATGCGGAAGAGAAGCCCCCTAGCGGAACCAAGGAAAAGAAGCACCAGTGCGACCACTGT GAAAGATGCTTCTACACCCGGAAGGATGTGCGACGCCACCTGGTGGTCCACACAGGATGCAAGGACTTC CTGTGCCAGTTCTGTGCCCAGAGATTTGGGCGCAAGGATCACCTCACCCGGCATACCAAGAAGACCCAC TCACAGGAGCTGATGAAAGAGAGCTTGCAGACCGGAGACCTTCTGAGCACCTTCCACACCATCTCGCCT TCATTCCAACTGAAGGCTGCTGCCTTGCCTCCTTTCCCTTTAGGAGCTTCTGCCCAGAACGGGCTTGCA AGTAGCTTGCCAGCTGAGGTCCATAGCCTCACCCTCAGTCCCCCAGAACAAGCCGCCCAGCCTATGCAG CCGCTGCCAGAGTCCCTGGCCTCCCTCCACCCCTCGGTATCCCCTGGCTCTCCTCCGCCACCCCTTCCC AATCACAAGTACAACACCACTTCTACCTCATACTCCCCACTTGCAAGCCTGCCCCTCAAAGCAGATACT AAAGGTTTTTGCAATATCAGTTTGTTTGAGGACTTGCCTCTGCAAGAGCCTCAGTCACCTCAAAAGCTC AACCCAGGTTTTGATCTGGCTAAGGGAAATGCTGGTAAAGTAAACCTGCCCAAGGAGCTGCCTGCAGAT GCTGTGAACCTAACAATACCTGCCTCTCTGGACCTGTCCCCCCTGTTGGGCTTCTGGCAGCTGCCCCCT CCTGCTACCCAAAATACCTTTGGGAATAGCACTCTTGCCCTGGGGCCTGGGGAATCTTTGCCCCACAGG TTAAGCTGTCTGGGGCAGCAGCAGCAAGAACCCCCACTTGCCATGGGCACTGTGAGCCTGGGCCAGCTC CCCCTGCCCCCCATCCCTCATGTGTTCTCAGCTGGCACTGGCTCTGCCATCCTGCCTCATTTCCATCAT GCATTCAGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001289045
Insert Size	1392 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289045.1
RefSeq Size	3709 bp
RefSeq ORF	1392 bp
Locus ID	5325
UniProt ID	Q9UM63
Protein Families	Transcription Factors
MW	50.8 kDa
Gene Summary	This gene encodes a C2H2 zinc finger protein that functions as a suppressor of cell growth. This gene is often deleted or methylated and silenced in cancer cells. In addition, overexpression of this gene during fetal development is thought to be the causal factor for transient neonatal diabetes mellitus (TNDM). Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding two different protein isoforms. The P1 downstream promoter of this gene is imprinted, with preferential expression from the paternal allele in many tissues. [provided by RefSeq, Nov 2015] Transcript Variant: This variant (16) initiates from the P1 promoter and differs in the 5' UTR compared to variant 2. Variants 2, 3, 4, 5, 6, 10, 12, 15, 16, 17, 18, 19, 20, 22, 23, 25, 27, and 28 all encode the same isoform (2).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.