BECN2 (NM_001290693) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BECN1L1; BECN1P1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336082 representing NM_001290693. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTTCCATCCGCTTCCTGTGCCAGCGCTGCCACCAGGCCCTGAAGCTGAGCGGCTCCTCGGAGTCT AGGAGCCTCCCTGCAGCCCCGGCGCCCACCTCTGGGCAGGCTGAGCCCGGAGACACCCGGGAGCCCGGC GTCACCACCAGGGAGGTGACAGACGCTGAGGAGCAACAGGACGGTGCCTCTAGCAGATCCCCTCCAGGC GATGGCAGTGTGTCCAAGGGCCATGCCAACATCTTCACCCTGCTGGGGGAGCTTGGCGCCATGCACATG CTCAGTAGCATCCAGAAGGCAGCTGGTGACATTTTTGACATAGTCTCTGGCCAAGCAGTTGTGGACCAT CCCCTGTGTGAAGAATGCACCGACAGTCTTTTAGAGCAGCTGGACATCCAGCTCGCTCTCACAGAAGCT GACAGTCAGAACTACCAACGCTGCCTGGAGACCGGGGAGCTGGCGACCAGCGAGGACGAGGCGGCGGCG CTGCGGGCGGAGCTGCGGGACCTGGAGCTGGAGGAGGCCAGGCTGGTGCAGGAGCTGGAGGATGTGGAC AGGAACAATGCAAGAGCAGCGGCGGATCTCCAGGCAGCCCAGGCAGAGGCTGCGGAGCTGGACCAGCAG GAGAGGCAGCACTACAGGGACTACAGTGCCTTGAAGCGGCAGCAGCTGGAACTGCTTGATCAGCTGGGG AACGTGGAGAACCAGCTGCAGTATGCCAGGGTCCAGAGGGACCGGCTGAAGGAAATCAACTGTTTCACC GCCACGTTTGAGATCTGGGTGGAGGGCCCCTTGGGCGTCATCAATAACTTCAGGTTGGGCCGCCTCCCC ACTGTCCGTGTGGGCTGGAATGAGATTAACACTGCCTGGGGACAGGCGGCCTTGCTGCTCCTTACCCTG GCCAATACAATTGGACTGCAGTTTCAGAGGTATCGACTCATCCCCTGCGGAAACCATTCGTATCTGAAG TCTTTAACAGATGACCGCACTGAGCTGCCGTTGTTCTGTTATGGGGGGCAGGATGTTTTCCTCAATAAC AAGTATGACCGCGCGATGGTGGCCTTCCTGGACTGCATGCAGCAGTTCAAGGAAGAGGCTGAGAAGGGT GAGCTGGGCCTCTCTCTGCCCTACGGGATCCAGGTGGAGACAGGCCTGATGGAGGACGTTGGCGGCCGA GGGGAATGCTATTCCATCAGAACCCATCTGAACACGCAGGAGCTGTGGACAAAGGCACTCAAGTTCATG CTTATAAATTTCAAGTGGAGTCTCATCTGGGTTGCCTCAAGGTATCAAAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001290693
Insert Size	1296 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290693.1
RefSeq Size	1296 bp
RefSeq ORF	1296 bp
Locus ID	441925
UniProt ID	A8MW95
Protein Pathways	Regulation of autophagy
MW	48.2 kDa
Gene Summary	Involved in 2 distinct lysosomal degradation pathways: acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. Regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1.[UniProtKB/Swiss-Prot Function]

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