DAAM1 (NM_001270520) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332963 representing NM_001270520. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCCCCAAGAAAGAGAGGTGGACGAGGTATTTCATTCATCTTTTGCTGTTTCCGAAATAATGATCAC CCAGAAATCACGTATCGGCTGCGAAATGATAGCAACTTTGCGCTTCAGACCATGGAACCAGCATTGCCC ATGCCCCCTGTGGAGGAGCTGGATGTCATGTTCAGTGAACTGGTGGATGAACTGGACCTCACAGACAAA CACAGAGAAGCCATGTTTGCACTTCCAGCTGAGAAAAAATGGCAAATATACTGTAGCAAGAAAAAGGAC CAGGAAGAAAACAAGGGAGCTACAAGTTGGCCTGAATTCTACATTGATCAGCTCAATTCCATGGCTGCT AGAAAATCTCTGCTGGCTTTAGAGAAGGAAGAAGAAGAAGAAAGAAGTAAAACTATAGAGAGTTTAAAG ACAGCACTGAGGACAAAACCAATGAGGTTTGTAACCAGATTCATCGACTTGGATGGCCTATCATGTATC CTCAACTTTCTAAAGACCATGGACTACGAGACCTCAGAGTCTCGAATACATACTTCTCTCATTGGCTGT ATAAAGGCGTTAATGAACAACTCTCAAGGCCGGGCTCACGTCCTGGCTCATTCTGAGAGTATTAATGTA ATTGCTCAGAGTCTGAGCACAGAGAACATTAAAACGAAGGTGGCCGTGCTGGAAATCTTGGGCGCCGTG TGCCTGGTTCCCGGGGGCCACAAGAAGGTTCTGCAGGCCATGCTGCACTACCAGAAGTATGCCAGCGAA AGGACCCGCTTTCAGACATTAATTAACGACTTGGATAAAAGCACTGGGCGGTATCGAGATGAAGTGAGT CTCAAGACTGCCATCATGTCCTTCATTAATGCAGTGCTCAGCCAAGGTGCAGGAGTGGAGAGTTTGGAC TTTAGACTTCATCTTCGCTATGAATTTCTGATGTTAGGAATTCAACCTGTAATAGATAAATTAAGGGAA CACGAAAATTCAACATTAGATAGGCATTTAGACTTTTTTGAAATGCTCCGAAATGAAGATGAACTAGAA TTTGCCAAAAGATTTGAACTGGTTCACATAGACACAAAAAGTGCAACTCAGATGTTTGAGCTGACCAGG AAGAGGCTGACACATAGTGAAGCTTACCCGCATTTCATGTCCATCCTGCACCACTGCCTCCAAATGCCT TACAAGAGGAGTGGCAACACTGTTCAGTACTGGCTACTACTAGATAGAATTATACAGCAGATAGTTATC CAGAATGACAAAGGACAGGACCCTGACTCCACACCTTTGGAAAACTTTAATATTAAGAATGTCGTACGA ATGTTGGTTAATGAAAATGAAGTTAAGCAGTGGAAAGAACAAGCGGAAAAAATGAGAAAAGAGCACAAT GAGCTACAACAGAAACTGGAAAAGAAAGAACGAGAATGTGATGCTAAGACTCAAGAGAAGGAAGAGATG ATGCAGACCTTAAATAAAATGAAAGAGAAACTTGAAAAGGAGACTACTGAGCATAAGCAAGTCAAGCAG CAGGTGGCGGACCTCACAGCACAGCTCCATGAGCTCAGCAGGAGGGCCGTCTGTGCTTCAATCCCAGGT GGACCCTCGCCTGGAGCACCAGGAGGGCCCTTTCCTTCCTCTGTGCCTGGATCTCTCCTTCCTCCCCCA CCACCCCCACCTCTACCAGGTGGGATGCTTCCCCCTCCACCGCCTCCCCTCCCTCCAGGTGGCCCTCCT CCTCCCCCAGGGCCTCCTCCCTTAGGGGCAATCATGCCACCTCCTGGTGCTCCAATGGGCCTAGCACTG AAGAAGAAAAGCATTCCTCAGCCCACAAATGCCCTGAAATCCTTCAACTGGTCTAAACTGCCCGAGAAC AAACTGGAAGGAACAGTATGGACCGAAATTGATGATACAAAAGTCTTCAAAATTCTAGATCTTGAAGAC CTGGAAAGAACCTTCTCTGCCTATCAAAGACAGCAGAAAGAAGCAGATGCCATTGATGACACTCTGAGT TCCAAACTTAAAGTTAAAGAGCTTTCGGTGATTGATGGTCGGAGAGCTCAGAATTGCAACATCCTTCTA TCGAGGTTGAAATTATCCAATGACGAAATCAAACGGGCAATTCTAACAATGGACGAACAGGAAGATCTG CCCAAGGACATGTTGGAACAGCTCTTGAAATTTGTTCCTGAAAAAAGTGACATTGACCTATTGGAGGAA CATAAACACGAACTGGATCGGATGGCCAAGGCTGATAGGTTCCTTTTTGAGATGAGCCGAATTAATCAC TATCAGCAAAGGTTGCAATCGCTGTACTTCAAAAAGAAGTTTGCAGAGCGTGTGGCAGAAGTGAAACCT AAAGTGGAAGCAATTCGTTCTGGCTCAGAAGAGGTGTTTAGGAGTGGTGCCCTCAAGCAGTTGCTGGAG GTGGTTTTGGCATTTGGAAATTATATGAATAAAGGTCAAAGAGGGAATGCATATGGATTCAAGATATCT AGCCTAAACAAAATTGCTGACACAAAATCCAGCATCGACAAAAACATTACCCTTTTGCACTATCTCATC ACTATTGTGGAAAATAAGTACCCCAGTGTTCTCAATCTAAATGAAGAATTGCGAGATATTCCTCAAGCT GCGAAAGTAAACATGACTGAGCTGGACAAAGAAATAAGTACCTTGAGAAGTGGCTTGAAAGCAGTAGAG ACAGAGCTGGAATATCAGAAGTCTCAGCCCCCACAGCCCGGAGATAAGTTTGTGTCTGTTGTCAGCCAG TTCATCACAGTAGCCAGCTTCAGCTTCTCTGATGTTGAAGACCTTCTAGCAGAAGCTAAAGACCTGTTT ACTAAAGCAGTGAAGCACTTTGGGGAAGAGGCTGGCAAAATACAACCAGATGAGTTCTTTGGCATTTTT GATCAATTTCTTCAAGCTGTGTCAGAAGCCAAACAAGAAAACGAAAATATGAGAAAGAAAAAGGAGGAA GAAGAACGTCGAGCTCGCATGGAAGCTCAGCTCAAAGAACAACGTGAAAGGGAACGTAAAATGAGAAAA GCTAAAGAGAATAGTGAAGAAAGCGGAGAGTTTGATGACCTTGTTTCAGCTTTACGCTCAGGAGAAGTG TTTGACAAAGACCTTTCTAAATTGAAACGGAATCGCAAACGTATTACCAACCAGATGACTGACAGCAGC AGAGAGAGACCAATCACAAAACTTAATTTCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270520
Insert Size	3207 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001270520.1
RefSeq Size	5896 bp
RefSeq ORF	3207 bp
Locus ID	23002
UniProt ID	Q9Y4D1
Protein Pathways	Wnt signaling pathway
MW	122.3 kDa
Gene Summary	Cell motility, adhesion, cytokinesis, and other functions of the cell cortex are mediated by reorganization of the actin cytoskeleton and several formin homology (FH) proteins have been associated with these processes. The protein encoded by this gene contains two FH domains and belongs to a novel FH protein subfamily implicated in cell polarity. A key regulator of cytoskeletal architecture, the small GTPase Rho, is activated during development by Wnt/Fz signaling to control cell polarity and movement. The protein encoded by this gene is thought to function as a scaffolding protein for the Wnt-induced assembly of a disheveled (Dvl)-Rho complex. This protein also promotes the nucleation and elongation of new actin filaments and regulates cell growth through the stabilization of microtubules. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1, and encodes a shorter isoform (2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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