POLD1 (NM_001256849) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CDC2; CRCS10; MDPL; POLD
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332489 representing NM_001256849. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGATGGCAAGCGGCGGCCAGGCCCAGGGCCCGGGGTGCCCCCAAAGCGGGCCCGTGGGGGCCTCTGG GATGATGATGATGCACCTCGGCCATCCCAATTCGAGGAGGACCTGGCACTGATGGAGGAGATGGAGGCA GAACACAGGCTGCAGGAGCAGGAGGAGGAGGAGCTGCAGTCAGTCCTGGAGGGGGTTGCAGACGGGCAG GTCCCACCATCAGCCATAGATCCTCGCTGGCTTCGGCCCACACCACCAGCGCTGGACCCCCAGACAGAG CCCCTCATCTTCCAACAGTTGGAGATTGACCATTATGTGGGCCCAGCGCAGCCTGTGCCTGGGGGGCCC CCACCATCCCGCGGCTCCGTGCCTGTGCTCCGCGCCTTCGGGGTCACCGATGAGGGGTTCTCTGTCTGC TGCCACATCCACGGCTTCGCTCCCTACTTCTACACCCCAGCGCCCCCTGGTTTCGGGCCCGAGCACATG GGTGACCTGCAACGGGAGCTGAACTTGGCCATCAGCCGGGACAGTCGCGGGGGGAGGGAGCTGACTGGG CCGGCCGTGCTGGCTGTGGAACTGTGCTCCCGAGAGAGCATGTTTGGGTACCACGGGCACGGCCCCTCC CCGTTCCTGCGCATCACCGTGGCGCTGCCGCGCCTCGTGGCCCCGGCCCGCCGTCTCCTGGAACAGGGC ATCCGTGTGGCAGGCCTGGGCACGCCCAGCTTCGCGCCCTACGAGGCCAACGTCGACTTTGAGATCCGG TTCATGGTGGACACGGACATCGTCGGCTGCAACTGGCTGGAGCTCCCAGCTGGGAAATACGCCCTGAGG CTGAAGGAGAAGGCTACGCAGTGCCAGCTGGAGGCGGACGTGCTGTGGTCTGACGTGGTCAGTCACCCA CCGGAAGGGCCATGGCAGCGCATTGCGCCCTTGCGCGTGCTCAGCTTCGATATCGAGTGCGCCGGCCGC AAAGGCATCTTCCCTGAGCCTGAGCGGGACCCTGTCATCCAGATCTGCTCGCTGGGCCTGCGCTGGGGG GAGCCGGAGCCCTTCCTACGCCTGGCGCTCACCCTGCGGCCCTGTGCCCCCATCCTGGGTGCCAAGGTG CAGAGCTACGAGAAGGAGGAGGACCTGCTGCAGGCCTGGTCCACCTTCATCCGTATCATGGACCCCGAC GTGATCACCGGTTACAACATCCAGAACTTCGACCTTCCGTACCTCATCTCTCGGGCCCAGACCCTCAAG GTACAAACATTCCCTTTCCTGGGCCGTGTGGCCGGCCTTTGCTCCAACATCCGGGACTCTTCATTCCAG TCCAAGCAGACGGGCCGGCGGGACACCAAGGTTGTCAGCATGGTGGGCCGCGTGCAGATGGACATGCTG CAGGTGCTGCTGCGGGAGTACAAGCTCCGCTCCTACACGCTCAATGCCGTGAGCTTCCACTTCCTGGGC GAGCAGAAGGAGGACGTGCAGCACAGCATCATCACCGACCTGCAGAATGGGAACGACCAGACCCGCCGC CGCCTGGCTGTGTACTGCCTGAAGGATGCCTACCTGCCACTGCGGCTGCTGGAGCGGCTCATGGTGCTG GTGAACGCCGTGGAGATGGCGAGGGTCACTGGCGTGCCCCTCAGCTACCTGCTCAGTCGTGGCCAGCAG GTCAAGGTCGTATCCCAGCTGTTGCGGCAGGCCATGCACGAGGGGCTGCTGATGCCCGTGGTGAAGTCA GAGGGCGGCGAGGACTACACGGGAGCCACTGTCATCGAGCCCCTCAAAGGGTACTACGACGTCCCCATC GCCACCCTGGACTTCTCCTCGCTGTACCCGTCCATCATGATGGCCCACAACCTGTGTTACACCACGCTC CTTCGGCCCGGGACTGCACAGAAACTGGGCCTGACTGAGGATCAGTTCATCAGGACCCCCACCGGGGAC GAGTTTGTGAAGACCTCAGTGCGGAAGGGGCTGCTGCCCCAGATCCTGGAGAACCTGCTCAGTGCCCGG AAGAGGGCCAAGGCCGAGCTGGCCAAGGAGACAGACCCCCTCCGGCGCCAGGTCCTGGATGGACGGCAG CTGGCGCTGAAGGTGAGCGCCAACTCCGTATACGGCTTCACTGGCGCCCAGGTGGGCAAGTTGCCGTGC CTGGAGATCTCACAGAGCGTCACGGGGTTCGGACGTCAGATGATCGAGAAAACCAAGCAGCTGGTGGAG TCTAAGTACACAGTGGAGAATGGCTACAGCACCAGTGCCAAGGTGGTGTATGGTGACACTGACTCCGTC ATGTGCCGATTCGGCGTGTCCTCGGTGGCTGAGGCGATGGCCCTGGGGCGGGAGGCCGCGGACTGGGTG TCAGGTCACTTCCCGTCGCCCATCCGGCTGGAGTTTGAGAAGGTCTACTTCCCATACCTGCTTATCAGC AAGAAGCGCTACGCGGGCCTGCTCTTCTCCTCCCGGCCCGACGCCCACGACCGCATGGACTGCAAGGGC CTGGAGGCCGTGCGCAGGGACAACTGCCCCCTCGTGGCCAACCTGGTCACTGCCTCACTGCGCCGCCTG CTCATCGACCGAGACCCTGAGGGCGCGGTGGCTCACGCACAGGACGTCATCTCGGACCTGCTGTGCAAC CGCATCGATATCTCCCAGCTGGTCATCACCAAGGAGCTGACCCGCGCGGCCTCCGACTATGCCGGCAAG CAGGCCCACGTGGAGCTGGCCGAGAGGATGAGGAAGCGGGACCCCGGGAGTGCGCCCAGCCTGGGCGAC CGCGTCCCCTACGTGATCATCAGTGCCGCCAAGGGTGTGGCCGCCTACATGAAGTCGGAGGACCCGCTG TTCGTGCTGGAGCACAGCCTGCCCATTGACACGCAGTACTACCTGGAGCAGCAGCTGGCCAAGCCCCTC CTGCGCATCTTCGAGCCCATCCTGGGCGAGGGCCGTGCCGAGGCTGTGCTACTGCGGGGGGACCACACG CGCTGCAAGACGGTGCTCACGGGCAAGGTGGGCGGCCTCCTGGCCTTCGCCAAACGCCGCAACTGCTGC ATTGGCTGCCGCACAGTGCTCAGCCACCAGGGAGCCGTGTGTGAGTTCTGCCAGCCCCGGGAGTCTGAG CTGTATCAGAAGGAGGTATCCCATCTGAATGCCCTGGAGGAGCGCTTCTCGCGCCTCTGGACGCAGTGC CAGCGCTGCCAGGGCAGCCTGCACGAGGACGTCATCTGCACCAGCCGGGACTGCCCCATCTTCTACATG CGCAAGAAGGTGCGGAAGGACCTGGAAGACCAGGAGCAGCTCCTGCGGCGCTTCGGACCCCCTGGACCT GAGGCCTGGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256849
Insert Size	3324 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256849.1
RefSeq Size	3467 bp
RefSeq ORF	3324 bp
Locus ID	5424
UniProt ID	P28340
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	Base excision repair, DNA replication, Homologous recombination, Metabolic pathways, Mismatch repair, Nucleotide excision repair, Purine metabolism, Pyrimidine metabolism
MW	123.6 kDa
Gene Summary	This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (1) contains an alternate exon in the 5' UTR and uses an alternate splice site in the 3' coding region, compared to variant 4. The encoded isoform (1) is shorter than isoform 2.

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