GUCY1A2 (NM_001256424) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GC-SA2; GUC1A2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332380 representing NM_001256424. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCTCGAAGGAAGATTTCGTCCGAGTCCTTCAGCTCCCTGGGCTCCGACTACCTGGAGACCAGCCCG GAGGAGGAGGGGGAGTGCCCCCTGTCTAGGCTCTGCTGGAATGGCAGCCGGAGCCCGCCCGGGCCGCTG GAGCCCAGCCCGGCCGCAGCTGCCGCTGCCGCCGCCCCGGCCCCGACCCCGGCTGCTTCTGCCGCCGCC GCCGCTGCCACTGCCGGGGCCAGGAGGGTGCAGCGCCGGAGGCGGGTCAACCTGGACTCGCTGGGCGAG AGCATCAGCCGCCTGACGGCGCCCTCGCCTCAGACGATACAGCAGACTCTCAAGAGGACACTGCAGTAT TATGAACATCAAGTTATTGGTTACAGGGATGCAGAAAAGAATTTCCACAATATCTCTAACAGATGCTCC TATGCAGACCACTCCAACAAAGAAGAAATTGAAGATGTCTCAGGAATTCTTCAGTGTACTGCTAATATA CTCGGTTTGAAGTTTGAGGAAATTCAAAAAAGATTTGGTGAAGAGTTCTTTAATATATGCTTTCATGAG AATGAGAGAGTCCTTCGAGCTGTAGGTGGCACTTTGCAGGACTTTTTTAACGGCTTTGATGCTTTGTTG GAACACATTAGAACTTCTTTTGGAAAACAGGCCACTCTGGAGTCACCATCTTTCCTATGCAAAGAGCTC CCTGAAGGTACTCTCATGCTCCACTACTTCCACCCTCACCATATTGTGGGGTTTGCAATGCTGGGGATG ATTAAGGCTGCAGGAAAGAAGATCTATCGGCTGGATGTGGAAGTGGAACAGGTTGCAAATGAGAAGCTA TGCTCTGATGTTTCAAACCCAGGCAATTGTAGCTGTCTTACTTTCCTTATCAAAGAATGTGAAAATACT AATATCATGAAGAACCTTCCACAGGGAACCTCCCAAGTTCCTGCGGACCTCAGAATTAGCATCAACACC TTCTGTAGAGCCTTCCCTTTCCACTTGATGTTTGATCCCAGCATGTCAGTCCTTCAGTTGGGGGAAGGT CTAAGGAAGCAGCTTCGATGTGACACTCACAAAGTGCTCAAGTTTGAGGACTGCTTCGAGATTGTATCT CCAAAGGTTAATGCCACCTTTGAAAGGGTCCTGCTGCGACTGTCTACCCCGTTTGTGATTAGAACCAAG CCTGAGGCTTCTGGCTCTGAAAATAAAGACAAGGTGATGGAAGTCAAAGGACAAATGATCCATGTTCCA GAATCAAATTCCATTTTATTTTTGGGCTCTCCATGTGTGGACAAGTTGGATGAACTCATGGGCCGAGGG CTACATCTCTCAGACATCCCTATCCATGATGCCACCCGAGATGTCATTTTGGTTGGTGAGCAGGCAAAG GCCCAAGATGGGTTGAAGAAAAGGATGGATAAATTAAAGGCAACTTTAGAAAGAACTCACCAGGCCCTG GAAGAAGAGAAAAAGAAGACAGTGGATCTTCTATATTCTATTTTCCCTGGTGATGTAGCCCAGCAATTA TGGCAAGGGCAGCAAGTACAGGCCAGAAAGTTTGATGATGTCACCATGCTCTTTTCAGACATTGTTGGC TTCACAGCCATATGTGCCCAGTGTACTCCCATGCAAGTAATCAGCATGCTGAATGAACTGTACACCAGA TTTGACCACCAGTGTGGATTTTTGGATATTTATAAGGTGGAAACAATAGGTGATGCCTACTGTGTTGCA GCAGGGCTCCACAGAAAAAGCCTCTGCCATGCTAAACCCATTGCTCTGATGGCCTTGAAGATGATGGAA CTTTCAGAAGAGGTGCTGACACCTGATGGAAGACCGATTCAGCCCCAGAGGAGTGAACTCCTTTTCTCC TTTCCTGTTTCCATCCAGCTGGTTCCAGATCAGCATCAAAGTGAAACGGATTTGGGTACTGAAAAGATG AGGATAGGAATTCACTCAGGCTCCGTGCTGGCTGGAGTTGTTGGGGTGCGAATGCCACGTTATTGCCTG TTTGGAAATAATGTCACACTGGCAAGCAAATTCGAGTCGGGAAGTCACCCTCGGCGCATCAATGTCAGC CCAACCACTTACCAATTATTAAAACGAGAAGAAAGTTTCACATTCATTCCGCGGTCTCGTGAAGAGCTT CCAGACAACTTTCCAAAGGAAATTCCTGGGATCTGCTATTTCCTGGAGGTAAGGACTGGTCCAAAGCCA CCAAAGCCTTCTCTTTCTTCGTCGAGAATAAAAAAGGTTTCCTACAACATCGGCACCATGTTCCTCCGG GAGACAAGCCTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256424
Insert Size	2292 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256424.1
RefSeq Size	16233 bp
RefSeq ORF	2292 bp
Locus ID	2977
UniProt ID	P33402
Protein Families	Druggable Genome
Protein Pathways	Gap junction, Long-term depression, Purine metabolism, Vascular smooth muscle contraction
MW	85.3 kDa
Gene Summary	Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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