GFPT1 (NM_001244710) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CMS12; CMSTA1; GFA; GFAT; GFAT 1; GFAT1; GFAT1m; GFPT; GFPT1L; MSLG
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332085 representing NM_001244710. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTGTGGTATATTTGCTTACTTAAACTACCATGTTCCTCGAACGAGACGAGAAATCCTGGAGACCCTA ATCAAAGGCCTTCAGAGACTGGAGTACAGAGGATATGATTCTGCTGGTGTGGGATTTGATGGAGGCAAT GATAAAGATTGGGAAGCCAATGCCTGCAAAATCCAGCTTATTAAGAAGAAAGGAAAAGTTAAGGCACTG GATGAAGAAGTTCACAAGCAACAAGATATGGATTTGGATATAGAATTTGATGTACACCTTGGAATAGCT CATACCCGTTGGGCAACACATGGAGAACCCAGTCCTGTCAATAGCCACCCCCAGCGCTCTGATAAAAAT AATGAATTTATCGTTATTCACAATGGAATCATCACCAACTACAAAGACTTGAAAAAGTTTTTGGAAAGC AAAGGCTATGACTTCGAATCTGAAACAGACACAGAGACAATTGCCAAGCTCGTTAAGTATATGTATGAC AATCGGGAAAGTCAAGATACCAGCTTTACTACCTTGGTGGAGAGAGTTATCCAACAATTGGAAGGTGCT TTTGCACTTGTGTTTAAAAGTGTTCATTTTCCCGGGCAAGCAGTTGGCACAAGGCGAGGTAGCCCTCTG TTGATTGGTGTACGGAGTGAACATAAACTTTCTACTGATCACATTCCTATACTCTACAGAACAGCTAGG ACTCAGATTGGATCAAAATTCACACGGTGGGGATCACAGGGAGAAAGAGGCAAAGACAAGAAAGGAAGC TGCAATCTCTCTCGTGTGGACAGCACAACCTGCCTTTTCCCGGTGGAAGAAAAAGCAGTGGAGTATTAC TTTGCTTCTGATGCAAGTGCTGTCATAGAACACACCAATCGCGTCATCTTTCTGGAAGATGATGATGTT GCAGCAGTAGTGGATGGACGTCTTTCTATCCATCGAATTAAACGAACTGCAGGAGATCACCCCGGACGA GCTGTGCAAACACTCCAGATGGAACTCCAGCAGATCATGAAGGGCAACTTCAGTTCATTTATGCAGAAG GAAATATTTGAGCAGCCAGAGTCTGTCGTGAACACAATGAGAGGAAGAGTCAACTTTGATGACTATACT GTGAATTTGGGTGGTTTGAAGGATCACATAAAGGAGATCCAGAGATGCCGGCGTTTGATTCTTATTGCT TGTGGAACAAGTTACCATGCTGGTGTAGCAACACGTCAAGTTCTTGAGGAGCTGACTGAGTTGCCTGTG ATGGTGGAACTAGCAAGTGACTTCCTGGACAGAAACACACCAGTCTTTCGAGATGATGTTTGCTTTTTC CTTAGTCAATCAGGTGAGACAGCAGATACTTTGATGGGTCTTCGTTACTGTAAGGAGAGAGGAGCTTTA ACTGTGGGGATCACAAACACAGTTGGCAGTTCCATATCACGGGAGACAGATTGTGGAGTTCATATTAAT GCTGGTCCTGAGATTGGTGTGGCCAGTACAAAGGCTTATACCAGCCAGTTTGTATCCCTTGTGATGTTT GCCCTTATGATGTGTGATGATCGGATCTCCATGCAAGAAAGACGCAAAGAGATCATGCTTGGATTGAAA CGGCTGCCTGATTTGATTAAGGAAGTACTGAGCATGGATGACGAAATTCAGAAACTAGCAACAGAACTT TATCATCAGAAGTCAGTTCTGATAATGGGACGAGGCTATCATTATGCTACTTGTCTTGAAGGGGCACTG AAAATCAAAGAAATTACTTATATGCACTCTGAAGGCATCCTTGCTGGTGAATTGAAACATGGCCCTCTG GCTTTGGTGGATAAATTGATGCCTGTGATCATGATCATCATGAGAGATCACACTTATGCCAAGTGTCAG AATGCTCTTCAGCAAGTGGTTGCTCGGCAGGGGCGGCCTGTGGTAATTTGTGATAAGGAGGATACTGAG ACCATTAAGAACACAAAAAGAACGATCAAGGTGCCCCACTCAGTGGACTGCTTGCAGGGCATTCTCAGC GTGATCCCTTTACAGTTGCTGGCTTTCCACCTTGCTGTGCTGAGAGGCTATGATGTTGATTTCCCACGG AATCTTGCCAAATCTGTGACTGTAGAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001244710
Insert Size	2100 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001244710.1
RefSeq Size	8703 bp
RefSeq ORF	2100 bp
Locus ID	2673
UniProt ID	Q06210
Protein Families	Protease
Protein Pathways	Alanine, aspartate and glutamate metabolism, Amino sugar and nucleotide sugar metabolism, Metabolic pathways
MW	78.8 kDa
Gene Summary	This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. [provided by RefSeq, Sep 2008] Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript for this combination of exons and introns was available for the full length of the gene. The extent of this transcript is supported by mouse accession AF334736.1 and PubMed ID: 11679416.

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