KCNQ3 (NM_001204824) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BFNC2; EBN2; KV7.3
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331612 representing NM_001204824. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAGCCTGCAGAACACGCCACGATGTTCCTGATTGTCCTGGGGTGCTTGATTCTGGCTGTCCTGACC ACATTCAAGGAGTATGAGACTGTCTCGGGAGACTGGCTTCTGTTACTGGAGACATTTGCTATTTTCATC TTTGGAGCCGAGTTTGCTTTGAGGATCTGGGCTGCTGGATGTTGCTGCCGATACAAAGGCTGGCGGGGC CGACTGAAGTTTGCCAGGAAGCCCCTGTGCATGTTGGACATCTTTGTGCTGATTGCCTCTGTGCCAGTG GTTGCTGTGGGAAACCAAGGCAATGTTCTGGCCACCTCCCTGCGAAGCCTGCGCTTCCTGCAGATCCTG CGCATGCTGCGGATGGACCGGAGAGGTGGCACCTGGAAGCTTCTGGGCTCAGCCATCTGTGCCCACAGC AAAGAACTCATCACGGCCTGGTACATCGGTTTCCTGACACTCATCCTTTCTTCATTTCTTGTCTACCTG GTTGAGAAAGACGTCCCAGAGGTGGATGCACAAGGAGAGGAGATGAAAGAGGAGTTTGAGACCTATGCA GATGCCCTGTGGTGGGGCCTGATCACACTGGCCACCATTGGCTATGGAGACAAGACACCCAAAACGTGG GAAGGCCGTCTGATTGCCGCCACCTTTTCCTTAATTGGCGTCTCCTTTTTTGCCCTTCCAGCGGGCATC CTGGGGTCCGGGCTGGCCCTCAAGGTGCAGGAGCAACACCGTCAGAAGCACTTTGAGAAAAGGAGGAAG CCAGCTGCTGAGCTCATTCAGGCTGCCTGGAGGTATTATGCTACCAACCCCAACAGGATTGACCTGGTG GCGACATGGAGATTTTATGAATCAGTCGTCTCTTTTCCTTTCTTCAGGAAAGAACAGCTGGAGGCAGCA TCCAGCCAAAAGCTGGGTCTCTTGGATCGGGTTCGCCTTTCTAATCCTCGTGGTAGCAATACTAAAGGA AAGCTATTTACCCCTCTGAATGTAGATGCCATAGAAGAAAGTCCTTCTAAAGAACCAAAGCCTGTTGGC TTAAACAATAAAGAGCGTTTCCGCACGGCCTTCCGCATGAAAGCCTACGCTTTCTGGCAGAGTTCTGAA GATGCCGGGACAGGTGACCCCATGGCGGAAGACAGGGGCTATGGGAATGACTTCCCCATCGAAGACATG ATCCCCACCCTGAAGGCCGCCATCCGAGCCGTCAGAATTCTACAATTCCGTCTCTATAAAAAAAAATTC AAGGAGACTTTGAGGCCTTACGATGTGAAGGATGTGATTGAGCAGTATTCTGCCGGGCATCTCGACATG CTTTCCAGGATAAAGTACCTTCAGACGAGAATAGATATGATTTTCACCCCTGGACCTCCCTCCACGCCA AAACACAAGAAGTCTCAGAAAGGGTCAGCATTCACCTTCCCATCCCAGCAATCTCCCAGGAATGAACCA TATGTAGCCAGACCATCCACATCAGAAATCGAAGACCAAAGCATGATGGGGAAGTTTGTAAAAGTTGAA AGACAGGTTCAGGACATGGGGAAGAAGCTGGACTTCCTCGTGGATATGCACATGCAACACATGGAACGG TTGCAGGTGCAGGTCACGGAGTATTACCCAACCAAGGGCACCTCCTCGCCAGCTGAAGCAGAGAAGAAG GAGGACAACAGGTATTCCGATTTGAAAACCATCATCTGCAACTATTCTGAGACAGGCCCCCCGGAACCA CCCTACAGCTTCCACCAGGTGACCATTGACAAAGTCAGCCCCTATGGGTTTTTTGCACATGACCCTGTG AACCTGCCCCGAGGGGGACCCAGTTCTGGAAAGGTTCAGGCAACTCCTCCTTCCTCAGCAACAACGTAT GTGGAGAGGCCCACGGTCCTGCCTATCTTGACTCTTCTCGACTCCCGAGTGAGCTGCCACTCCCAGGCT GACCTGCAGGGCCCCTACTCGGACCGAATCTCCCCCCGGCAGAGACGTAGCATCACGCGAGACAGTGAC ACACCTCTGTCCCTGATGTCGGTCAACCACGAGGAGCTGGAGAGGTCTCCAAGTGGCTTCAGCATCTCC CAGGACAGAGATGATTATGTGTTCGGCCCCAATGGGGGGTCGAGCTGGATGAGGGAGAAGCGGTACCTC GCCGAGGGTGAGACGGACACAGACACGGACCCCTTCACGCCCAGCGGCTCCATGCCTCTGTCGTCCACA GGGGATGGGATTTCTGATTCAGTATGGACCCCTTCCAATAAGCCCATTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001204824
Insert Size	2259 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001204824.1
RefSeq Size	10717 bp
RefSeq ORF	2259 bp
Locus ID	3786
UniProt ID	O43525
Protein Families	Druggable Genome, Ion Channels: Potassium, Transmembrane
MW	84.8 kDa
Gene Summary	This gene encodes a protein that functions in the regulation of neuronal excitability. The encoded protein forms an M-channel by associating with the products of the related KCNQ2 or KCNQ5 genes, which both encode integral membrane proteins. M-channel currents are inhibited by M1 muscarinic acetylcholine receptors and are activated by retigabine, a novel anti-convulsant drug. Defects in this gene are a cause of benign familial neonatal convulsions type 2 (BFNC2), also known as epilepsy, benign neonatal type 2 (EBN2). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014] Transcript Variant: This variant (2) contains an alternate 5' terminal exon and it thus differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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