5HT3E (HTR3E) (NM_001256613) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	5-HT3-E; 5-HT3c1; 5-HT3E
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330468 representing NM_001256613. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAAGGAAGCTGGTTCCACAGGAAAAGATTTTCCTTCTACCTCCTTCTCGGTTTTCTGCTTCAAGGA AGGGGCGTTACTTTCACCATCAATTGCTCAGGGTTTGGCCAGCACGGGGCGGATCCCACTGCTCTGAAT TCAGTGTTTAATAGAAAGCCCTTCCGTCCGGTCACCAACATCAGCGTCCCCACCCAAGTCAACATCTCC TTCGCGATGTCTGCCATCCTAGATGTGAATGAACAGCTGCACCTCTTGTCATCATTCCTGTGGCTGGAA ATGGTTTGGGATAACCCATTTATCAGCTGGAACCCAGAGGAATGTGAGGGCATCACGAAGATGAGTATG GCAGCCAAGAACCTGTGGCTCCCAGACATTTTCATCATTGAACTCATGGATGTGGATAAGACCCCAAAA GGCCTCACAGCATATGTAAGTAATGAAGGTCGCATCAGGTATAAGAAACCCATGAAGGTGGACAGTATC TGTAACCTGGACATCTTCTACTTCCCCTTCGACCAGCAGAACTGCACACTCACCTTCAGCTCATTCCTC TACACAGTGGACAGCATGTTGCTGGACATGGAGAAAGAAGTGTGGGAAATAACAGACGCATCCCGGAAC ATCCTTCAGACCCATGGAGAATGGGAGCTCCTGGGCCTCAGCAAGGCCACCGCAAAGTTGTCCAGGGGA GGCAACCTGTATGATCAGATCGTGTTCTATGTGGCCATCAGGCGCAGGCCCAGTCTCTATGTCATAAAC CTTCTCGTGCCCAGTGGCTTTCTGGTTGCCATCGATGCCCTCAGCTTCTACCTGCCAGTGAAAAGTGGG AATCGTGTCCCATTCAAGATAACGCTCCTGCTGGGCTACAACGTCTTCCTGCTCATGATGAGTGACTTG CTCCCCACCAGTGGCACCCCCCTCATCGGTGTCTACTTCGCCCTGTGCCTGTCCCTGATGGTGGGCAGC CTGCTGGAGACCATCTTCATCACCCACCTGCTGCACGTGGCCACCACCCAGCCCCCACCCCTGCCTCGG TGGCTCCACTCCCTGCTGCTCCACTGCAACAGCCCGGGGAGATGCTGTCCCACTGCGCCCCAGAAGGAA AATAAGGGCCCGGGTCTCACCCCCACCCACCTGCCCGGTGTGAAGGAGCCAGAGGTATCAGCAGGGCAG ATGCCGGGCCCTGCGGAGGCAGAGCTGACAGGGGGCTCAGAATGGACAAGGGCCCAGCGGGAACACGAG GCCCAGAAGCAGCACTCAGTGGAGCTGTGGTTGCAGTTCAGCCACGCGATGGACGCCATGCTCTTCCGC CTCTACCTGCTCTTCATGGCCTCCTCTATCATCACCGTCATATGCCTCTGGAACACCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001256613
Insert Size	1371 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256613.1
RefSeq Size	2154 bp
RefSeq ORF	1371 bp
Locus ID	285242
UniProt ID	A5X5Y0
Protein Families	Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
MW	51.4 kDa
Gene Summary	This locus encodes a 5-hydroxytryptamine (serotonin) receptor subunit. The encoded protein, subunit E, may play a role in neurotransmission in myenteric neurons. Genes encoding subunits C, D and E form a cluster on chromosome 3. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Feb 2012] Transcript Variant: This variant (4) has multiple differences in the 5' UTR and coding region, compared to variant 1. These differences result in translation initiation at an alternate start codon, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a.

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